Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,665,661 G→A 5.0% E77K (GAA→AAA)  csiE → stationary phase inducible protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,665,6610GA 5.0% 274.9 / 2.1 89E77K (GAA→AAA) csiEstationary phase inducible protein
Reads supporting (aligned to +/- strand):  ref base G (42/38);  new base A (3/2);  total (48/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

CAAACTTTAGCTATTCTTATCAATTATGCTTATGGGAGATCTACAGATGATGCCTACGCTTGCTCCACCATCTGTCCTTTCGGCTCCCCAGCGCCGCTGCCAGATCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAATCGCGCGATATTCATTTCCTGTGTCTGTATCTGCAATATTGTTTGCTGCAGCACCACGCTGGAA  >  NC_000913/2665387‑2665931
                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                               
cAAACTTTGACTATTCTTATCAATTATGCTTATGGGAGATCTACAGATGCTGCCTACGCTTGCTCCACCATCTGTCCTTTCGGCTCCCCAGCGCCGCTGCCAGATCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTg                                                                                                                                                                                                                                                                                 <  1:475771/275‑1 (MQ=255)
      tttgCTATTCTTATCAAGTATGTGTCTGGGAGATCTACACAGGATGCCTACGCTTGCTCCAACATCTTTCCTTTCGGCTCCCCAGCGCCGCGTCCAGATCGTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATTGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTa                                                                                                                                                                                                                                                                          <  1:569258/272‑1 (MQ=255)
      ttATCTATTCGTATCAAGTATGCTTACGGGGGATTTACCGATGGTGCCTACGCGTGGCCCACCATGTGTCCTTTCGGCACCCCAGAGCCGCTGCCAGATCTTGCTGACGCTCGTTCAGACGGGGTTGGCCGCAACCATGGCAACCTTTAGCGAGCTTAAGGGTGTGGAGGATGATATTGCCAGTCTTGATACCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTa                                                                                                                                                                                                                                                                          <  1:270187/275‑1 (MQ=255)
       tAGCTATTCTTATCAATTATGCTTATGGGAGATCTACAGATGATGCCTACGCTTGCTCCACCATCTGTCCTTTCGGCTCCCCAGCGCCGCTGCCAGATCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTAc                                                                                                                                                                                                                                                                         <  2:683898/275‑1 (MQ=255)
               cTTATCAATTATGCTTATGGGGGATCTACAGATGAGGCCTACGCTTGCTCCACCATCTGTCCTTTCGGCTCCCCAGCGCCGCTGCCAGATCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCt                                                                                                                                                                                                                                                                   <  2:147668/273‑1 (MQ=255)
               cTTATCAATTATGCTTATGGGAGATCTACAGATGATGCCTACGCTTGCTCCACCATCTGTCCTTTCGGCTCCCCAGCGCCGCTGCCAGCTCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGCTCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTGTAAGGTACAGTGCTTa                                                                                                                                                                                                                                                                 >  1:308267/1‑275 (MQ=255)
                 tATCAATTATGCTTATGGGAGATCTACAGATGATGCCTACGCTTGCTCCACCATCTGTCCTTTCGGCTCCCCAGCGCCGCTGCCAGATCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAAc                                                                                                                                                                                                                                                               <  2:169810/275‑1 (MQ=255)
                       ttATGCTTATGGGAGATCTACAGATGATGCCTACGCTTGCTCCACCATCTGTCCTTTCGGCTCCCCAGCGCCGCTGCCAGATCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACg                                                                                                                                                                                                                                                          >  1:469365/1‑274 (MQ=255)
                         aTGCTTATGGGAGATCTACAGATGATGCCTACGCTTGCTCCACCATCTGTCCTTTCGGCGCCCCAGCGCCGCTGCCAGATCTGGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGttt                                                                                                                                                                                                                                                       <  1:486939/275‑1 (MQ=255)
                               aTGGGAGATCTACAGATGATGCCTACGCTTGCTCCACCATCTGTCCTTTCGGCTCCCCAGCGCCGCTGCCAGATCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTCCAGTGCTTAACCAACGTTTGTGttt                                                                                                                                                                                                                                                 >  1:592509/1‑275 (MQ=255)
                               aTGGGAGATCTACAGATGATGCCTACGCTTGCTCCACCATCTGTCCTTACGCTTCCCCATCGCCGCTGCCAGATCCTGCAGACGCCCTTTCAGCTGGGGTTGACCGACACCTTGTCAACCTTCAGCGAGCCTAATGGTGTGGATGATGATATTCCTAGTCTTGATATCAGCGAATCAGGACGGGAGACCCTGCGCTATCATCACCTCCCACTGACGACTGCTTATGACGGTAGCTACAG‑‑‑‑‑‑‑GTCTTAAAGTCCAACGTTCAACCAACGTTAGTGtt                                                                                                                                                                                                                                                  >  1:219810/1‑274 (MQ=255)
                                      aTCTACAGATGATGCCTACGCTTGCTCCACCATCTGTCCTTTCGGCTCCCCAGCGCCGCTGCCAGATCTTGCTGCCGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTCGCTACCG‑‑‑‑‑‑‑GGTTTAAGGTACAGGCCTTAACCAACGTCTGTGTTTATTTAAc                                                                                                                                                                                                                                          >  2:424193/1‑275 (MQ=255)
                                      aTCTACAGATGATGCCTACGCTTGCTCCACCATCTGTCCTTTCGGCTCCCCAGCGCCGCTGCCAGATCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGCGATCCTGCGCTATCATCAACTCACCCTGACGACTGGTTAGGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTAAAAGTGTTAACCAACCTTTGTGTTTATGTCAc                                                                                                                                                                                                                                          >  1:372996/1‑275 (MQ=255)
                                                 atgCCTACGCTTGCTCCACCATCTGTCCTTTCGGCTCCCCAGCGCCGCTGCCAGATCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGCTATTGCCAGTCTTGATATCAGCGAAACCGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGGGTTTATTTCACTGGCTacgacg                                                                                                                                                                                                                               >  2:317593/1‑275 (MQ=255)
                                                   gCCTACGCTTGCTCCACCATCTGTCCTTTCGGCTCCCCAGCGCCGCTGCCAGATCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGTTCCAGTGCTTAACCAACGTTTGTGTTTCTTTCACTGGCTACGACGTg                                                                                                                                                                                                                             >  2:217121/1‑275 (MQ=255)
                                                   gCCTACGCTTGCTCCACCATCTGTCCTTTCGGCTCCCCAGCGCCGCTGCCAGATCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGt                                                                                                                                                                                                                              >  1:816615/1‑274 (MQ=255)
                                                   gCCTACGCTTGCTCCACCATCTGTCCTTTCGGCTCCCCAGCGCCGCTGCCAGATCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGACTGATTATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATACACTCACACTGACGACTGGTTATGACGGTCGCTACCG‑‑‑‑‑‑‑TGTTGAAGGTACAGTGCTTAACAACTGTTTGTGTTTATTTCCGTGGCTACGACGtt                                                                                                                                                                                                                             >  2:422899/1‑274 (MQ=255)
                                                   gCCTACGCTTGCTCCACCATCTGTCCTTTCGGCTCCCCAGCGCCGCTGCCAGATCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATCGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTCCCG‑‑‑‑‑‑‑AGTTGAAGGTACAGTGCTTAACAACCGTTTGTGTTTATTTCACTGGCTCCGCCGt                                                                                                                                                                                                                              >  2:432480/1‑274 (MQ=255)
                                                     cTACGCTTGCTCCACCATCTGTCCTTTCGGCTCCCCAGCGCCGCTGCCAGATCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCATGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTGGCTACCG‑‑‑‑‑‑‑GGTTAAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGt                                                                                                                                                                                                                           >  1:667130/1‑275 (MQ=255)
                                                     cTACGCATGCTCCACCATCTGTCCTTTCGGCTCCCCAGCGCCGCTGCCAGATCTTGCTGGCGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATTACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGt                                                                                                                                                                                                                           <  2:554591/275‑1 (MQ=255)
                                                              cTCCACCATCTGTCCTTTCGGCTCCCCAGCGCCGCTGCCAGATCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCtg                                                                                                                                                                                                                  >  1:492826/1‑275 (MQ=255)
                                                              cTCCACCATCTGTCCTTTCGGCTCCCCAGCGCCGCTGCCAGATCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCtg                                                                                                                                                                                                                  >  1:267491/1‑275 (MQ=255)
                                                                     aTCTGTCCTGTCGGCTCCCCAGCGCCGCTGCCAGATCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGt                                                                                                                                                                                                           <  2:816616/275‑1 (MQ=255)
                                                                         gTCCTTTCGGCTCCCCAGCGCCGCTGCCAGATCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGCAGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTTTTTCACTGGCACCGACGGGTTTTCCGTCTGTGGCCGTCCtt                                                                                                                                                                                                       >  1:858861/1‑275 (MQ=255)
                                                                                    tCCCCAGCGCCGCTGCCAGATCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGc                                                                                                                                                                                             >  1:355378/1‑274 (MQ=255)
                                                                                         aGCGCCGCTGCCAGATCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAAtt                                                                                                                                                                                        <  2:492827/274‑1 (MQ=255)
                                                                                           cgccgcTGCCAGATCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCCTCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGTTACAGTTCTTAACCAACGTTTGTGTTTATTTCACTGGCTATGACGTGGTTTCCGACTGTGTCTGTCTTTTATTACCAGCCAATTctc                                                                                                                                                                                     >  1:708239/1‑273 (MQ=255)
                                                                                                           tGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTAtt                                                                                                                                                                                                                                              >  1:462672/1‑202 (MQ=255)
                                                                                                           tGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAcgag                                                                                                                                                                      >  1:408018/1‑274 (MQ=255)
                                                                                                               gACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCAc                                                                                                                                                                                                                                          <  2:462673/202‑1 (MQ=255)
                                                                                                                  gCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTCTCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTCCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCCTTTATTACCAGCCAATTCACCCCAGCCCTGAAGAGTTAACTgc                                                                                                                                                              >  2:765898/1‑274 (MQ=255)
                                                                                                                  gCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTg                                                                                                                                                               <  2:355379/274‑1 (MQ=255)
                                                                                                                                ggTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAAt                                                                                                                                                 >  2:74630/1‑274 (MQ=255)
                                                                                                                                              aTGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACtttt                                                                                                                                   <  1:134782/274‑1 (MQ=255)
                                                                                                                                                ggCAACCGTAAGCGAGCTTAATGGGGTGGATGGTGAGATTGCCAGTCTTGATATCAGCGAAACAGGACGGAAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTac                                                                                                                                 <  2:200707/274‑1 (MQ=255)
                                                                                                                                                    aCCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTAcgacga                                                                                                                            <  2:478742/275‑1 (MQ=255)
                                                                                                                                                     ccTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTacgacgtt                                                                                                                           >  2:224712/1‑273 (MQ=255)
                                                                                                                                                         cAGCGGGCTTAATGGGGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATAAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCa                                                                                                                       <  1:524773/275‑1 (MQ=255)
                                                                                                                                                          aGCGAGCTTAATGGTGTGGATGATGATCTTGCCAGTCTTGATATCAGCGAAACAGGCCGGGAGATCCTGCGCTATCATCAACTCACAATGACGACTGGTTATGACGGGAACCACCG‑‑‑‑‑‑‑GGTTGAAGCTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCTTTTATTACCAGGCAATTCCCCGCCGCCCTGAAGTGTCAACTGATCCTGCCCGGCATTGACCTTAACTTTTACCAAGATatcc                                                                                                                       >  1:200707/1‑271 (MQ=255)
                                                                                                                                                            cGAGCTTAAGGGTGTGGATGGTGATATTGCCAGTCTTGAGATCAGCGAAACAGGGCGGGAGATCCTGCGCTATCATGAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTGTATTTCACTGGCTACGACGTGGTTTCCGTCGGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCa                                                                                                                       <  2:219810/272‑1 (MQ=255)
                                                                                                                                                                cTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTCTCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTACGTCCTTTCTTACCAGCCAATTCACCCCCGCCCTGAAGCGTGAATTGAAGCGGCGCGGAATTGCCGGTAACTTTTACCGCGATACCAATCTa                                                                                                                  >  2:373186/1‑273 (MQ=255)
                                                                                                                                                                cTTAATGGTGTGGATGATGATATTGCCAGTCTTGATAACAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTTGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTAc                                                                                                                 <  1:430069/274‑1 (MQ=255)
                                                                                                                                                                attaagtgtgtGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATGCTGAGCTATCATCAACTCACACTGAAGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTAc                                                                                                                 <  1:424192/267‑1 (MQ=255)
                                                                                                                                                                 ttAATGGTGTGGATGATGATATTGCCAGGCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACa                                                                                                                <  2:267491/274‑1 (MQ=255)
                                                                                                                                                                   aaTGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCGACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACaa                                                                                                               >  2:220189/1‑273 (MQ=255)
                                                                                                                                                                    aTGGTGTGGATGATGATATTGCCAGTCATGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTGGCTACCG‑‑‑‑‑‑‑GGTTAAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCTCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCg                                                                                                            <  2:667131/275‑1 (MQ=255)
                                                                                                                                                                       gtgtGGATGATGATATTGCCCGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCGACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTAc                                                                                                                 <  1:220189/267‑1 (MQ=255)
                                                                                                                                                                           ggATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTCCGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGAGCGGAATTGCGCGTCACTTTTACGACGCTACCACTCTACAAGCGTTAGTg                                                                                                      >  2:156444/1‑274 (MQ=255)
                                                                                                                                                                               gatgatATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAAt                                                                                                   <  2:469366/273‑1 (MQ=255)
                                                                                                                                                                                       tGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAAc                                                                                                                                                                                                                                                           >  1:162918/1‑113 (MQ=255)
                                                                                                                                                                                       tGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTCCGACGATACCAATCTACAAGCGTTCGTGAATCTCTGcccc                                                                                          >  1:848320/1‑274 (MQ=255)
                                                                                                                                                                                       tGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTATGCTcc                                                                                          >  2:561171/1‑274 (MQ=255)
                                                                                                                                                                                           aGTCTTGATATCAGCGAAACAGGAGGGGGGATCCTGCGCAATCATCAACTCACACGGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTACCCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCGGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGAc                                                                                      <  1:750653/274‑1 (MQ=255)
                                                                                                                                                                                           aGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGttt                                                                                                                                                                                                                                                       <  2:162918/113‑1 (MQ=255)
                                                                                                                                                                                           aGTCTTAATATCAGCGAAACAGGGCGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGATGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCCCTGGCTACGCCGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGAc                                                                                      <  2:708240/274‑1 (MQ=255)
                                                                                                                                                                                                 gATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCAc                                                                                                                                                                                     >  1:358143/1‑173 (MQ=255)
                                                                                                                                                                                                 gATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGCATTGCGCGTAACTTTTACGACGATACCAAACAACAAGGCTTCGTGAATCTCTGCTCCCGACGGCTGCa                                                                               >  1:678463/1‑275 (MQ=255)
                                                                                                                                                                                                  atatCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGTAACCAATCTACAAACGTTAGAGAATCCCTGACCCCGACGGCTGCa                                                                               >  1:707284/1‑274 (MQ=255)
                                                                                                                                                                                                    attcgCGAAACAGGACGGGAGATCCTGCGCGATCATCAAATCGCACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGGCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCaaaa                                                                            <  1:156444/271‑1 (MQ=255)
                                                                                                                                                                                                    agcAGCGAAACAGGACGGGAGATCCTGCGATATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGGCGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCaaaa                                                                            <  2:694585/273‑1 (MQ=255)
                                                                                                                                                                                                     tCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCAccccc                                                                                                                                                                                 <  2:358144/173‑1 (MQ=255)
                                                                                                                                                                                                         cGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAg                                                                                                                                                                                                                                                                              >  2:797332/1‑77 (MQ=255)
                                                                                                                                                                                                         cGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTAAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTCCCAGCCAATTCCCCCCCGCCCTGAAGCGTGAACTGAAGCGGCGCGGAATTGCGCGGACCTTTTACGACGATACCAATCTACAAGCGTTATTGAATCTCGGCTCCCGACGCCTGCaaaacccct                                                                       >  1:335628/1‑270 (MQ=255)
                                                                                                                                                                                                            aaCAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCttt                                                                     >  2:741060/1‑274 (MQ=255)
                                                                                                                                                                                                             aCAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTAc                                                                                                                                                                                                                                                                         <  1:797331/77‑1 (MQ=255)
                                                                                                                                                                                                                   cGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAATcg                                                               >  1:652014/1‑273 (MQ=255)
                                                                                                                                                                                                                    gggAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAATcgcgc                                                            <  2:408019/275‑1 (MQ=255)
                                                                                                                                                                                                                      gagaTCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCAGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACGGAAGCGGCACGGAATTGCGCGTAACTTTTACGACGATACCAATCTACCAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACTGTTTGAATcgcgc                                                            >  2:858779/1‑273 (MQ=255)
                                                                                                                                                                                                                      gagaTCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTAAAGGTACAGTGCTTAACAAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGACGCGGCGCGGTATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCACTGCTACCGACGGCTGCAAAAACGACTTGAATACCGCGa                                                          >  2:324483/1‑275 (MQ=255)
                                                                                                                                                                                                                        cttccttcccgatcatcAACCCACACTGACGACTGGTTATGACGTTAGCTCCCG‑‑‑‑‑‑‑GGTTGAGGGTACCGTGCTTAACAAACGTTTGTTTTTATTTCACTGCCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGCCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAATCGCGCGa                                                          <  2:858862/262‑1 (MQ=255)
                                                                                                                                                                                                                          tCCTGCTCCATCATCAACTCACCCTGACGATTGGTTTTGCCGGTAGCTACCG‑‑‑‑‑‑‑GGTTAAAGGGACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTGCGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAATcgc                                                              <  1:858778/267‑1 (MQ=255)
                                                                                                                                                                                                                                 cTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGGACAGTGCTTAACCAACGTGTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAATCGCGCGATATTCATTTc                                                <  2:372997/274‑1 (MQ=255)
                                                                                                                                                                                                                                    tcatcaACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAATCGCGCGATATTCATTTCCtgt                                            >  2:191839/1‑275 (MQ=255)
                                                                                                                                                                                                                                    tcatcaACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAATCGCGCGATATTCATTTCCtgt                                            >  2:727648/1‑275 (MQ=255)
                                                                                                                                                                                                                                         aaCTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGCAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGCGAATCTCTGCTCCCGACGGCTGCAAAACCGCTTTGAATCGCGCGATATTCATTTCCtgtctacg                                       >  2:870253/1‑270 (MQ=255)
                                                                                                                                                                                                                                         aaCTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGAt                                                                                                                           >  2:36672/1‑191 (MQ=255)
                                                                                                                                                                                                                                          aCTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTAAAGGGCTTAACCAACGTTTGTGTTGATTTCACTGGCTACGACGTGGTTTCCTGCTGTGTCCGTCAGTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAATCGCGCGATATTCATTTCCTGTGTCTGt                                      <  1:217121/275‑1 (MQ=255)
                                                                                                                                                                                                                                           cTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAATCGCGCGATATTCATTTCCtgt                                            >  2:660050/1‑275 (MQ=255)
                                                                                                                                                                                                                                             cacaCTGACGACTGGTTATGGCGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCa                                                                                                                       <  1:36672/191‑1 (MQ=255)
                                                                                                                                                                                                                                              acacTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAAt                                                                 >  2:672499/1‑244 (MQ=255)
                                                                                                                                                                                                                                                acTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAATCGCGCGATATTCATTTCCTGTGTCTGTATCTg                                 >  2:52808/1‑274 (MQ=255)
                                                                                                                                                                                                                                                  tGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAATcgcg                                                             <  1:672498/244‑1 (MQ=255)
                                                                                                                                                                                                                                                    acgacTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGAGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAAACGCGCAATATTCATTTCCTGTGTCTGTATCTGCAata                            >  2:554374/1‑275 (MQ=255)
                                                                                                                                                                                                                                                         tGGTTATGACGGTGGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAATCGCGCGATATTCATTTCCTGTGTCTGTATCTGCAATATTGtt                       <  1:432479/275‑1 (MQ=255)
                                                                                                                                                                                                                                                         tGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAATCGCGCGATATTCATTTCCTGTGTCTGTATCTGCAATATTGtt                       <  1:191839/275‑1 (MQ=255)
                                                                                                                                                                                                                                                          ggTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGACGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAATCGCGTGATATTCATTTCCTGGGTCTGTATCTGCAATATTGtt                       >  2:112319/1‑274 (MQ=255)
                                                                                                                                                                                                                                                           gTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTTAGGGTACAGTTGTTACCCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTGTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAATCGCGCGATATTCATTTCCTGTGTCTGTATCTGCAATATTGtt                       <  1:317593/273‑1 (MQ=255)
                                                                                                                                                                                                                                                                    gTAGTTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCCCTGGCTACGACGTGGTGTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAATCGCGCGATATTCATTTCCTGTGTCTGTATCTGCAATATTGTTTGCTGCAGcac            <  2:308267/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                           ccG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAATCGCGCGATATTCATTTCCTGTGTCTGTATCTGCAATATTGTTTGCTGCAGCACCACGCt      <  2:652015/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     ggTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAATCGCGCGATATTCATTTCCTGTGTCTGTATCTGCAATATTGTTTGCTGCAGCACCACGCTGGaa  >  2:191853/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                               
CAAACTTTAGCTATTCTTATCAATTATGCTTATGGGAGATCTACAGATGATGCCTACGCTTGCTCCACCATCTGTCCTTTCGGCTCCCCAGCGCCGCTGCCAGATCTTGCTGACGCTCTTTCAGCCGGGGTTGACCGCCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCG‑‑‑‑‑‑‑GGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATACCAATCTACAAGCGTTAGTGAATCTCTGCTCCCGACGGCTGCAAAAACGCTTTGAATCGCGCGATATTCATTTCCTGTGTCTGTATCTGCAATATTGTTTGCTGCAGCACCACGCTGGAA  >  NC_000913/2665387‑2665931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: