Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,746,201 A→T 3.7% T37S (ACC→TCC)  yiaO → 2,3‑diketo‑L‑gulonate‑binding periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,746,2010AT 3.7% 343.6 / 2.9 109T37S (ACC→TCC) yiaO2,3‑diketo‑L‑gulonate‑binding periplasmic protein
Reads supporting (aligned to +/- strand):  ref base A (63/39);  new base T (2/2);  total (68/41)
Fisher's exact test for biased strand distribution p-value = 6.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01

Only 100 of 111 total aligned reads displayed.

ATGTACTTAACGTTATTTCAGGGGTGGCAAAACTCAAATTCGATGATGCAGTCAGAGGCGTTTTCCCTTACGTCCTGGTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCT  >  NC_000913/3745929‑3746475
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                  
aTGTACTCAACGTTATTTCAGGGGTGGCAAAACTCAAATTCGATGATGCAGTCAGAGGCGTTTTTCCTTACGTCGTGGTTTTCTATTCATAAATAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTCCGCTCTGTAACCTACCCATATTACATCGCTGGCCTGGCTGCATTAGGCACCACTCCCCCGGCCGCCCAACCTTTACGTTTCGTCTATTAAACATCACCAAcc                                                                                                                                                                                                                                                                                  >  1:14847/1‑275 (MQ=255)
 tGTACTTAACGTTATTTCAGGGGTGGCAAAACTCAAATTCGATGATGCAGTCAGAGGCGTTTTCCCTTACGTCCTGGTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCg                                                                                                                                                                                                                                                                                 <  1:662139/275‑1 (MQ=255)
 tGTACTTAACGTTATTTCAGGGGTGGCAAAACTCAAATTCGATGATGCAGTCAGAGGCGTTTTCCCTTACGTCCTGGTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCg                                                                                                                                                                                                                                                                                 <  2:356868/275‑1 (MQ=255)
 tGTACTTAACGTTATTTCAGGGGTGGCAAAACTCAAATTCGATGATGCAGTCAGAGGCGTTTTCCCTTACGTCCTGGTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCg                                                                                                                                                                                                                                                                                 >  1:374220/1‑275 (MQ=255)
   tccTTAAGGTGATTTCAGGGGTGGCAAAACTCAACTGCGAGGATGCAGTCAGAGGTGTTTTCTCTTACGGCCTGGTTTTATACTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTGATCATCCTACCTTGGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGAc                                                                                                                                                                                                                                                                               <  1:729019/273‑1 (MQ=255)
      ttAACGTTATTTCAGGGGTGGCAAAACTCAAATTCGATGATGCAGTCAGAGGCGTTTTCCCTTACGTCCTGGTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACCTCACAAACCGACTcc                                                                                                                                                                                                                                                                            >  2:584548/1‑274 (MQ=255)
        aaCGTTATTTCAGGGGTGGCAAAACTCAAATTCGATGATGCAGTCAGAGGCGTTTTCCCTTACGTCCTGGTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCCCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCCCAAACCGACTCGCa                                                                                                                                                                                                                                                                          >  1:316713/1‑275 (MQ=255)
             tATTTCAGGGGTGGCAAAACTCAAATTCGATGATGCAGTCAGAGGCGTTTTCCCTTACGTCCTGGTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGCAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACCCGCAACata                                                                                                                                                                                                                                                                     >  1:583466/1‑275 (MQ=255)
             tATTTCAGGGGTGGCAAAACTCAAATTCGATGATGCAGTCAGAGGCGTTTTCCCTTACGTCCTGGTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGACACATCACAACCCGACCCGCAACata                                                                                                                                                                                                                                                                     >  2:225098/1‑275 (MQ=255)
             tATTTCAGGGGTGGCAAAACTCAAATTCGATGATGCAGTCAGAGGCGTTTTCCCTTACGTCCTGGTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACata                                                                                                                                                                                                                                                                     >  1:73620/1‑275 (MQ=255)
               tttCAGGGGTGGCAAAACTCAAATTCGATGATGCAGTCAGAGGCGTTTTCCCTTACGTCCTGGTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATAACAAACCGCCTCGAAAAATAtt                                                                                                                                                                                                                                                                   >  1:545933/1‑275 (MQ=255)
                          gCAAAACTCAAATTCGATGATGCAGTCAGAGGCGTTTTCCCTTACGTCCTGGTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCCCAAACCGACTCGCAACATATTGCGGCGaaaaa                                                                                                                                                                                                                                                        >  2:193396/1‑275 (MQ=255)
                               aCTCAAATTCGATGATGCAGTCAGAGGCGTTTTCCCTTACGTCCTGGTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCGCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACATACCGACTCGCAACATATTGCGGCGAAAAAATTCa                                                                                                                                                                                                                                                   <  2:384082/275‑1 (MQ=255)
                                cTCAAATTCGATGATGCAGTCAGAGGCTTTTTCCCTTACGTCCTGGTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCaa                                                                                                                                                                                                                                                  <  2:345425/275‑1 (MQ=255)
                                    aaTTCGATGATGCAGTCAGAGGCGTTTTCCCTTACGTCCTGGTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCCCAAACCGAATCGAAACATATTGCGGCAGAAAAATTAAATGAt                                                                                                                                                                                                                                              >  1:767258/1‑275 (MQ=255)
                                    aaTTCGATGATGCAGTCAGAGGCGTTTTCCCTTACGTCCTGGTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGAATTATTCATTGCTGGCCTGGATGCATTCAGCACATCTTCTCTGGCGGCCCAATCTTTACGTTTCGGTTATGAAACATCACAACCCGACTCGCAACATATTGCGGCGAAAAAATTCAATGAt                                                                                                                                                                                                                                              >  2:359519/1‑275 (MQ=255)
                                      ttCGATGATGCTGTCAGAGGCGTTTTCCCTTACGTCCTGGTTTTATATTTATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTCCGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCGGCACACCTTCTCTGGCCGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGAtt                                                                                                                                                                                                                                             <  2:545934/274‑1 (MQ=255)
                                          atgatgCAGTCAGAGGCGTTTTCCCTTACGTCCTGGTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTg                                                                                                                                                                                                                                        <  1:469802/275‑1 (MQ=255)
                                                               tCCCTTACGTCCTGGTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGaaaaa                                                                                                                                                                                                                                                        >  1:410297/1‑238 (MQ=255)
                                                                   ttACGTCCTGGTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTc                                                                                                                                                                                                                                                    <  2:410298/238‑1 (MQ=255)
                                                                         ccTGGTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTaa                                                                                                                                                                                                         >  1:164150/1‑275 (MQ=255)
                                                                            ggTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGAAAAATATTGCGGCGAAAACATTCACTGATTTCTTGCAGGAGAGAACAAAAGGCGAGCTGAAATTaaaa                                                                                                                                                                                                       >  2:373808/1‑274 (MQ=255)
                                                                             gTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAAAACCAAAGGCGAGCGGAAATTAacac                                                                                                                                                                                                      >  1:85987/1‑274 (MQ=255)
                                                                                ttATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAAt                                                                                                                                                                                                                                                 >  2:763259/1‑228 (MQ=255)
                                                                                  atatTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAACCTGTTcc                                                                                                                                                                                                >  2:451583/1‑275 (MQ=255)
                                                                                   tatTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGAttt                                                                                                                                                                                                                                            >  1:517841/1‑230 (MQ=255)
                                                                                   tatTCATTAGTAGTCGTATTTTTTTTTATTCCCAATCTCATCATCCTCCCTTTGAAATTGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTccc                                                                                                                                                                                               <  1:343201/275‑1 (MQ=255)
                                                                                    atTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGAtt                                                                                                                                                                                                                                             <  1:763258/228‑1 (MQ=255)
                                                                                       cATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGCAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCACCATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGa                                                                                                                                                                                            >  2:127043/1‑274 (MQ=255)
                                                                                       cATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTg                                                                                                                                                                                                                                        <  2:517842/230‑1 (MQ=255)
                                                                                       cATTAATAGTCGTATTTGTATTAAATCCCGATCTCATCATCCTCACTTTGACATGAATTAATTAAAAAGAAAATATTATGAAATTACGCTCTGTAACATACGCATTAGTCAGTGCTGTCCTGGATGAATTAAGCACATCTTCTCTGGCGACACAATCTTTAAGTTTCGTTTATAAACAATCAACAACCGACTCGCAACATATTACGGCGAAAAAATTCAATGATTTATTGCAGGAGAGCACCAAAGGCGCGCTGCAATTAAAAATGTCCGCggcc                                                                                                                                                                                           >  1:110382/1‑273 (MQ=255)
                                                                                        attattAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCCCAATCCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGAc                                                                                                                                                                                           >  2:453427/1‑274 (MQ=255)
                                                                                        attattAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACa                                                                                                                                                                                          <  2:392230/275‑1 (MQ=255)
                                                                                         ttattaGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACATACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCTCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGCAATTAAAACTGTTCCCGGACa                                                                                                                                                                                          >  2:475261/1‑274 (MQ=255)
                                                                                                tCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACtctc                                                                                                                                                                                  >  2:246542/1‑275 (MQ=255)
                                                                                                   tATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAAATGTTCCCg                                                                                                                                                                                              >  1:392229/1‑260 (MQ=255)
                                                                                                         tttttATTCCCTGTTTCATCATCCTCCCTTTAAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGATCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAAcgcg                                                                                                                                                                         <  1:438278/275‑1 (MQ=255)
                                                                                                             tATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACACTCTTTACGTTTCGGTTATGAAACATCCCAAACCGACTAGCAACATATTGCGGCGAAAAACTTCAAAGCTTTATTGCAGGAGAGAACCAAAGGCGAGCGAAAATTAAAACTGTTCCCGGAAAGCAACCCCGGTacgcagacgg                                                                                                                                                                     >  1:433028/1‑266 (MQ=255)
                                                                                                                              acctccCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTTGCCTGGTTGCATTCAGCACATCTTCTCTGGCCGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTa                                                                                                                                                    <  1:645668/274‑1 (MQ=255)
                                                                                                                                    cTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAACCCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCGGGACAGCACTCTCGGTAACGCGCAGGCGATGCTCAGCGGCGTACGTggc                                                                                                                                              >  2:812504/1‑275 (MQ=255)
                                                                                                                                     tttGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTggcg                                                                                                                                             >  2:205320/1‑275 (MQ=255)
                                                                                                                                      ttGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAATCCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTggcgg                                                                                                                                            <  2:8092/275‑1 (MQ=255)
                                                                                                                                       tGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAATCCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGACCAGCGGCGTACGTg                                                                                                                                                >  1:8092/1‑270 (MQ=255)
                                                                                                                                            tGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCAt                                                                                                                                      <  1:246542/275‑1 (MQ=255)
                                                                                                                                            tGGAGTAATTAAAAGGAAAATATTATGAAATTACGGTTGTTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCAt                                                                                                                                      <  2:3658/275‑1 (MQ=255)
                                                                                                                                             ggATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACt                                                                                                                                                                                                     >  1:364217/1‑211 (MQ=255)
                                                                                                                                             ggATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGAGAGCTGAAATTAAAACTGTTCCCGGACCGCACTCTCGGTAACGCGCAGGCGATGAACAGAGGCGTACGTGGCGGCACCAt                                                                                                                                      >  1:403197/1‑274 (MQ=255)
                                                                                                                                               attaattaAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCCCAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGCTGATCAGCGGCGTACGTGGCGGCACCacgac                                                                                                                                   >  2:115838/1‑271 (MQ=255)
                                                                                                                                                 taattaaAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTc                                                                                                                                                                                                 <  2:364218/211‑1 (MQ=255)
                                                                                                                                                             aaaTATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCCCGGTAACGCGCAGGCGATGATCAGCGGCGTAAGTGGCGGCAACCTCGATAGTGAAATgcacg                                                                                                                     >  2:715643/1‑271 (MQ=255)
                                                                                                                                                              aaTATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCCCGGTAACGCGCAGGCGCTGATAAGCGGCGTACGTGGCGGCACCATCGATATGGGAATGTCCg                                                                                                                     >  2:779870/1‑274 (MQ=255)
                                                                                                                                                               atatTTAGAAATTACGCTCTTTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGc                                                                                                                   <  1:465437/275‑1 (MQ=255)
                                                                                                                                                                         aTTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACCGCACTCTCGGTAACGCCCAGGCGATGATCAGCGGCTGACGTGGCGGCACCCTCGATATGGAAATGTCCGGCCCGAATAACt                                                                                                         >  1:467578/1‑275 (MQ=255)
                                                                                                                                                                              gCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGcc                                                                                                    <  1:205320/275‑1 (MQ=255)
                                                                                                                                                                               agctGAAACCTACGAAGTATTCATTGCTGGCCTGGCTGAATTCAGCACATCTTCTCGGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGcc                                                                                                    <  1:366776/272‑1 (MQ=255)
                                                                                                                                                                                  tGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGACAAGTCCGGCTCGAATAACTTTCCCGGGt                                                                                                >  1:116285/1‑275 (MQ=255)
                                                                                                                                                                                     aaCCGACGAATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTATACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTAt                                                                                             <  2:221828/275‑1 (MQ=255)
                                                                                                                                                                                        cTACGCATTATTCATTGTTGGCCTGGTTGTATTACGCACATCTTCTCTGGCGGCAAAATCTTTACGTTTCGGTTATGAAACATCACAATCCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCAc                                                                                          <  1:453426/275‑1 (MQ=255)
                                                                                                                                                                                          aCGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGCTGACAAGCGGCGTACGAGGCGGAACCATCGATATGGAAATGTCCGGCCAGACAAACTTTGCCGGGTTCACACCa                                                                                        >  1:5157/1‑275 (MQ=255)
                                                                                                                                                                                          aCGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGCTATGGAAATGTCCGGCTCGAATAACTTTCCCGGGTTATCACCa                                                                                        >  2:183601/1‑275 (MQ=255)
                                                                                                                                                                                          aCGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGAACAGCACTCTAGATCACGCCCAGGCGATGATCAGCGGCGTACGTGGCAGCACCATCGATATGGAAATGTACGGCTCGAACAACTTTGCAGGGtgacaacc                                                                                         >  1:421751/1‑267 (MQ=255)
                                                                                                                                                                                            gCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCCACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGCTCAGCGGCGTACGTGGCGGCACCACCGATATGGAACTGTCCGCGCCGAGTAACTTTGCCGGGTTATCACCTGt                                                                                      >  1:223237/1‑275 (MQ=255)
                                                                                                                                                                                            gCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGt                                                                                      >  2:492126/1‑275 (MQ=255)
                                                                                                                                                                                            gCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGAGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGACTAACTTTGCAGGGTTagccacatc                                                                                      >  2:224091/1‑267 (MQ=255)
                                                                                                                                                                                            gCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCCCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCAccct                                                                                       >  2:366777/1‑272 (MQ=255)
                                                                                                                                                                                            gCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCACGGTAACGCGCAGGCGATGATCAGCGGAGTACCTGGCGGCACCATCGATCTGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCCGt                                                                                      >  1:865641/1‑275 (MQ=255)
                                                                                                                                                                                                  ttCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGaa                                                                                <  2:146359/275‑1 (MQ=255)
                                                                                                                                                                                                  ttCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCCGGAATAACTTTTCCGGGTTAACCCCAgggt                                                                                    >  1:470436/1‑268 (MQ=255)
                                                                                                                                                                                                     aTTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAActt                                                                             <  2:374221/275‑1 (MQ=255)
                                                                                                                                                                                                       tGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGTCAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCCCCAGTGACGAActtg                                                                            >  2:379080/1‑274 (MQ=255)
                                                                                                                                                                                                            gcctggcTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCCCTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATAGGGAAATGTCCGGCTTGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTCCTTGa                                                                       >  2:479951/1‑274 (MQ=255)
                                                                                                                                                                                                            gcctggcTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGCTCCGCGGCGTACGTGGCGGCACCATAGACATGGACATGTCCGGCCCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTTCTTGAt                                                                      >  1:756961/1‑275 (MQ=255)
                                                                                                                                                                                                            gcctggcTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGa                                                                       >  2:350666/1‑274 (MQ=255)
                                                                                                                                                                                                            gcctggcTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCAGCGATACGGAAATGTCCGGCCCGAATAACTTTTCCGGGTTATCACCAGTGCAGACATTGCTTGAt                                                                      >  1:270558/1‑275 (MQ=255)
                                                                                                                                                                                                             cccggcggcCTTCAGCAAACTTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCTCAACATATTGCGGGGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATg                                                                     <  1:812503/272‑1 (MQ=255)
                                                                                                                                                                                                                 gcTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCCCCATCGGTATGGAAATGGCCGGCCCGAATCAATTTGCCGGGCTATCACCAggtcataaatttcttgctgtgcc                                                                 >  1:57334/1‑253 (MQ=255)
                                                                                                                                                                                                                 gcTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTccc                                                                 >  2:355798/1‑275 (MQ=255)
                                                                                                                                                                                                                 gcTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGAGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTccc                                                                 >  2:796753/1‑275 (MQ=255)
                                                                                                                                                                                                                      aTTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGAttt                                                                                                                                                                                                                                            >  1:130747/1‑99 (MQ=255)
                                                                                                                                                                                                                          aGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTg                                                                                                                                                                                                                                        <  2:130747/99‑1 (MQ=255)
                                                                                                                                                                                                                                 cttctCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGAt                                                 >  2:496808/1‑275 (MQ=255)
                                                                                                                                                                                                                                      ctGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAAATTTGCCGGGTTATCACCAGTGATTAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGc                                            >  2:536801/1‑275 (MQ=255)
                                                                                                                                                                                                                                          cggcACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCAACGATATGGAAATGTCCGGCCCGAATAACTTTGCCGGTTTATCACCAGTGATGACCTTCATTGATGTCCCTTTCCTGTTCCGCGATACCGCCCa                                         >  1:845893/1‑274 (MQ=255)
                                                                                                                                                                                                                                           ggcACAGTCTTGACGTTTCGGTTATTAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAACCTTTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCAcg                                       <  2:223237/275‑1 (MQ=255)
                                                                                                                                                                                                                                           ggcACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCa                                         <  1:359518/273‑1 (MQ=255)
                                                                                                                                                                                                                                            gcACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGAt                                                                      >  2:120605/1‑243 (MQ=255)
                                                                                                                                                                                                                                                aaTCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTcc                                                                  <  1:120605/243‑1 (MQ=255)
                                                                                                                                                                                                                                                         gTTTCGGTTATGAAACATCACAAACCGCATCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGGGAACCAAAGGCGAGCTGAAATTAAACCTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTc                         <  1:373807/275‑1 (MQ=255)
                                                                                                                                                                                                                                                         gTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCt                          <  2:116285/274‑1 (MQ=255)
                                                                                                                                                                                                                                                            tCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTTGTCCGCCATACCGCCCCCCCGCATAAAACGCTCGAc                      >  2:3026/1‑275 (MQ=255)
                                                                                                                                                                                                                                                            tCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGAc                      >  2:566737/1‑275 (MQ=255)
                                                                                                                                                                                                                                                              ggTTATGAAACATCACAAACCGACTCGCAACATATTGCGGTGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGTCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGATCATGCGCATAAAACGATCGAAgg                    >  2:372804/1‑275 (MQ=255)
                                                                                                                                                                                                                                                              ggTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCCAAGAAAACGCCCGCCgg                    >  1:101050/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                ttATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCa                  <  2:73620/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                    taaaaatccaaaaCCGACGCGAAACAAATTGGGGCGAAAAAATTAAATGATTTATTGCAGGAGAGAACAAAAGGCGAGCTGAAATTAAAACGGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACTTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGt              <  1:451582/265‑1 (MQ=255)
                                                                                                                                                                                                                                                                          tCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACAAGTGATGAACTTGCTTGATGTCCATTTCCTGTTCCGCGATAACGCTAACGCGAATCAAACGCTCACCGGCACAGTCGGtg         >  2:552919/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                           cacaAACCGACTCGCAACATATTTCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTGCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGtgat       <  1:397650/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                             caAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGGTCGACGGCAAAGTACGggatga     >  2:605017/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                             caAACCCACTCGGAAAATATGGGGGCGAAAAAATTCAATGATTTAGTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGtgatg      <  1:350665/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                                aCCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAACACGCTCGACGGCAAAGTCGGTGATGATCt  >  2:358155/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                  
ATGTACTTAACGTTATTTCAGGGGTGGCAAAACTCAAATTCGATGATGCAGTCAGAGGCGTTTTCCCTTACGTCCTGGTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGATTAATTAAAAGGAAAATATTATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATCTTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCT  >  NC_000913/3745929‑3746475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: