Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,296,059 C→T 9.8% intergenic (+266/+378) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,0590CT 9.8% 131.7 / 7.9 67intergenic (+266/+378)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (37/22);  new base T (3/4);  total (41/26)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

AATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAT  >  NC_000913/4295789‑4296267
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                
aataatTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGcc                                                                                                                                                                                                               <  2:145068/274‑1 (MQ=255)
      taggtcggataaggcgtttacgccgcatccgacatcAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATg                                                                                                                                                                                                          <  2:190793‑M1/237‑1 (MQ=255)
         aGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACACCGCAATAGCCGGATTTAACTGGCTTTTGTAGGTCGGATAATGCTTTTACCCCGCATCCGCAATCAAACCCTgctctgaac                                                                                                                                                                                                     >  2:372442/1‑267 (MQ=14)
          gATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTTGGATAAGGCGTTTACGCAGCATCCGACATCAACGCCTGATGCGACg                                                                                                                                                                                                     >  2:805791/1‑274 (MQ=255)
          gATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATCCgcaga                                                                                                                                                                                                    >  2:722795/1‑271 (MQ=255)
             tGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAGTCCGCATCCGACATCAATTCCCGATGCGACTCtt                                                                                                                                                                                                  >  1:553489/1‑274 (MQ=255)
                   ggggttcgtccgacgcaggtacaaacgCCGGATGGGACGCTTGCGGCGTCTTATCAGGCCTCCGCCGTACAGCACAATAGCCTGTGTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAGGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCGACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt                                                                                                                                                                                           <  1:355282/249‑1 (MQ=1)
                   ggATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt                                                                                                                                                                                           <  2:115951/275‑1 (MQ=255)
                     atatCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCGGGCCTACGCCAGACAGCGCACTAGCCTGATTTAGCGTGATTTTGTAGGTCGGAAAACGCGTTTACGCCGCATACGACATCAACGCATGATGCGACGCTTGCCGCGTc                                                                                                                                                                                          >  2:763683/1‑274 (MQ=255)
                        tacgccgcatccgacatcAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTAAGCCAGACAGCGCAATAGCCTGCTTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCACTCCctgatgcgccgctttgcgcgcctact                                                                                                                                                                                      <  1:803655‑M1/257‑13 (MQ=255)
                        tacgccgcatccgacatcAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCAAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTCGGTCGGATAAGGCGTTTACGCCGCATCCGACCTCAATGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                                                                                       <  2:570223‑M1/256‑1 (MQ=255)
                         caacctcgcaagtgttggaggCCTAATGCTGCGTTGGCCGGTTCTTATCAGGCTGACGCCAGCCAGCGCGATAGCCTTATTTGGCATGATTTTTTAGGTCGGATATGGCGTTTTTTCCGCAGACGGCATCAACTCCTGATGCGACGCGTAACGCGTCTTATCAGGCCGACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGTCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                                                                                      <  1:467324/254‑1 (MQ=11)
                             gcgggaagttgtgaggGCGTGATGGGACGGTTGCGGGGTATGATCGGGCCTACGCCGAAGAGCGAAATAGCCGGATTTAGCGGGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                 >  1:418074‑M1/17‑275 (MQ=255)
                             cggtcaattgtgtAATGCCGGATGGGAGTCTTCCCGCGGTTTATCAGGCCTCGGCCAGAGAGAGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGTGTAAGGCGTTTGTGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACGGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                 >  1:192189‑M1/14‑275 (MQ=255)
                             cgcatcgcgaatcagcgaCTGATGGGACGCGTGCCGCGTCTTATCAGGCCTACGCCAGAGAGCGCAATAGCCTGATTTAGCGTGATTATGTAGGTCGGATAAGGCGTTTATGCCGCATCAGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                 >  1:227963‑M1/19‑275 (MQ=255)
                             ccGGCAATGTGTGAATGCCTGATTCGACGCTTGCCGTGTCGTATCTGGCTTAGGCAAGACAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACTCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                 <  2:564665/275‑1 (MQ=255)
                             ccGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGAAAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACTGCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                 >  2:850992/1‑275 (MQ=255)
                              ggggagggtgggAAGGCCTGGTGCGACGCGTGCCGCGGCTTGTCAGGCCGAGGCCAGAAAGGGAAAGAGCCGGATTTAGCGTGATTTTGTGGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                 <  1:162434/267‑1 (MQ=11)
                              gcatccgacatcAGCGCCTGAGGCGACGCTTGCCGCGTCTTATCAGTCCTACGCCAGGAGGCGCAATAGCCTGATGTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAATGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                                                                                                                >  2:553490‑M1/13‑275 (MQ=255)
                              cggcaaggttggAAGGCCTGATGCGACGCTTGCCGGGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCTGATAAGGCGTTTATGCCGCATCGGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                 >  1:806698‑M1/13‑274 (MQ=255)
                              cGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                 <  2:136859/274‑1 (MQ=39)
                                    tgtgtgAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTCTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGCTGCGACGCTTGACGAGTCTTCTCAGGCCTACGc                                                                                                                                                                           >  2:644301/1‑274 (MQ=19)
                                    tgtgtgAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGTCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTCCGc                                                                                                                                                                           >  2:241891/1‑274 (MQ=37)
                                    tgtgtgAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCATACGCCAGACATCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATACGACATCAACGCCTGCTGCGACGATTGACGCGTTTTATCAGGCCTACGc                                                                                                                                                                           >  2:622289/1‑274 (MQ=255)
                                      tgtgAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTCTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCa                                                                                                                                                                         >  2:864469/1‑274 (MQ=25)
                                       gggatggccggtggggaagCTTGGCGGGTCGTATCAGGCGTACGCGAGACAGCCAGTGAGCCTGATGTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAGGCCGGATCTGAAATCAACGCCTGATAGGACGCTTAACGCGTCTTATCAGGCTGACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGa                                                                                                                                                                       <  1:571958/257‑1 (MQ=11)
                                                TGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTCTGCCGCATCCGACATCAACGCCTGATGCGACGATTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGCCATCAACGCCTGATGCGACGCTTGCAGCGTCTTACCAGGCCTACGCCAGacagcgccat                                                                                                                                                              <  2:153917‑M1/275‑4 (MQ=255)
                                                TGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCaa                                                                                                                                                               >  2:723036‑M1/1‑274 (MQ=255)
                                                   TGCGAGGCTTGCCGCGTCTTTTCAGGCCTACGCCAGTCAGCGCAATAGCCTGATTTAGCGTTATTTTGTAGGTCGGATAAGGCGGTTATGCCGCATCCGACATCAACGCCTGATGCTACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc                                                                                                                                                           >  2:714435‑M1/1‑275 (MQ=255)
                                                                            gcagacgacatgccgcgcAGTAGCCTGATTAGGGTTGTGTTTGTAGGTGGGATAGGGCGTTTATGCCGCACGCGGGATCAACGCCTGATCGGACGCTTACCGCGTCTTATCAGGCCTACGCCAGAAAGCAAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGt                                                                                                                                   <  1:329049/260‑1 (MQ=11)
                                                                                           cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCCTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTCTCAGGCCTACGCCCGACAGCGCACTACCCCTATTCAGGCTGATCTTGTTAGTCGGGTACGGCCtttgg                                                                                                                   >  1:627878/1‑273 (MQ=11)
                                                                                           cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                                                                                                                >  1:315385/1‑214 (MQ=31)
                                                                                           cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCCCAACCGGCGCAATAGCCTTATTTCGCTTGATTTTGTAGGTCGTATACGGCGTTTac                                                                                                                   >  1:157730/1‑274 (MQ=11)
                                                                                            gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGATTCTTATCAGTCCTACGCATGCCAGCGCAATAGCCTGATTTAGCGTGACTTTGATAGTCGGATAAGGCTTTTATg                                                                                                                  >  2:201216/1‑275 (MQ=11)
                                                                                                      tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGACGCGTCTTATCAGTCCTACCCCATCCAGCGCATTAGCCTGCTTACGCTTTATTTCTTGTATCGGATAAGGCGcttccgcctcttcct                                                                                                         >  1:34313/1‑263 (MQ=11)
                                                                                                      tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTAAGACAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGAGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCCATCGCCTGATTCAGCGTGATTTTGTAGGTCGGCTAAGGCGTTCATCCCGCATCCg                                                                                                         >  2:319145/1‑275 (MQ=255)
                                                                                                        aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGGGTTTATGCTGCATCCGa                                                                                                        >  2:784481/1‑274 (MQ=21)
                                                                                                              cGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                                                                                                                     >  2:534225/1‑191 (MQ=31)
                                                                                                                  aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                 <  1:534224/191‑1 (MQ=31)
                                                                                                                           gTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACg                                                                                    >  1:727877/1‑275 (MQ=35)
                                                                                                                                 tAAGGGGTTTATGCCGCCTCCACCATCAACGCCTGGTGCTGCGCTTAACGCGTCTTATCAGGCCTAGGCCAGACAGCGCAATAGCCTGATTCATCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAc                                                                              <  2:268567/275‑1 (MQ=11)
                                                                                                                                 tAAGCCGTTTATGCCGCAGCCGTCATCAACGCCGGAGGCGACGCTTAAGTCTTCTTATCAGGCCTACGCCAGACAGCCAAATGGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTaa                                                                               <  1:805790/274‑1 (MQ=11)
                                                                                                                                         ttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTTATTTAGCGTGATTTTGTATGTCGGATAACGCGTTTACGCCGCCTCCGACCTCCACCTCTGCTGCGCCCCTTACCGCGTCtt                                                                       >  1:441706/1‑274 (MQ=11)
                                                                                                                                               cgaatcgggcagtaaCGCGTGATGGGACGCTTGACGGGTTGGATCAGGCTAACGCAGGACAGCGGAAGAGCATGATTTCGGGTGATTTGTTAGGTCGGAACAGGCGTTTACGCCGAAGCCGCCATCAACGCCTGATGCGACGCTTGCCGTGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                <  1:457717/262‑1 (MQ=11)
                                                                                                                                                gggtCCGAAATGAAGGCCTGATGCGACGCTTACCGCGTCTTATAAGGCCTACGCCCGACGGGGTAATAGCCTGATTTAGCGTGATTTTGTGGGTCGGTTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                <  1:680585/271‑1 (MQ=11)
                                                                                                                                                     ataCATCAACGCCGGAGTCGACGGTTCACGGACGTTATCAGGCCTGCGCCAGACAGCGCAATATCCTGATTTAGGTTGATTTTGTAGGTCGGATAGGGCGTTTACGCCCCGTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACg                                                           <  1:850991/272‑1 (MQ=11)
                                                                                                                                                         aTCAACGCCGGACGCGACACTTAACTGGGCTGATCAGGCCCACGCGAGATGGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGa                                                      <  2:797863/275‑1 (MQ=11)
                                                                                                                                                                 ggaaTGCGACGTTTCACGGGTCTTATCGGGGCGAGGCCAGGCAGGTCAATAGCCGGATTTAGCTTGATTTTGTGGGTCGGATAGGGCGTTTACGCCGCACCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCaa                                              <  1:136875/272‑1 (MQ=11)
                                                                                                                                                                 gagacgggACGCTTGCCGCGGCTTATCAGGCGTAGGCCAGACAGCGCAATTGCCTAATTTCGCGTGATTTTGTAGGTCGGATAAGGCTTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCaa                                              <  1:208421/268‑1 (MQ=11)
                                                                                                                                                                   gATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCTAGACAGCGCATTAGCCTGATTTCGCGTGATTTTGTAGGTCGGATAACGCGTTTACGCCGCATCCGACATCAGCGCCTGATGAGACGCTTAACGCCTCTTATCAGGCTTACGCAAGACAgcggctaa                                            >  1:85774/1‑270 (MQ=11)
                                                                                                                                                                                      cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCCATAGCCTTATTTCGCGTGAttt                         >  2:573186/1‑275 (MQ=18)
                                                                                                                                                                                      cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAttt                         >  1:872617/1‑275 (MQ=33)
                                                                                                                                                                                      cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGACAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTCGCCGCGTCTTATCCGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGAGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCCGGCCTACGCCAGACAGCGAAATAGCCTGATATAGCGTGAttt                         >  2:619305/1‑275 (MQ=255)
                                                                                                                                                                                             ggccgacccccgccaggctaaTAGCCGGTTTTAGCTTGATTTTTGTGGTCGGATAAGGCGTTTCGGCCCCATCCGACATCAACGCCGGATGCGACGCTGGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGt                  <  1:864468/256‑1 (MQ=11)
                                                                                                                                                                                              gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGAATCCGACATCAACGCCTGATGCCACGCTTGACGCGTCTTATCAGGCCTACTCCAGCCAGCGCCATAGCCTGACTTCGCTTGactccgtatctc                 >  1:328141/1‑264 (MQ=11)
                                                                                                                                                                                              gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTACCAGGCCTACGCCAACCACCGCAATAGCCTTCTTTCCCTTCATTTTGTAGGt                  >  1:572623/1‑274 (MQ=11)
                                                                                                                                                                                                ctcggCCAGACAGCGCAATAGCCTTATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGGATCCGACATCAACGCCTGATGGGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCgg               <  1:722794/271‑1 (MQ=18)
                                                                                                                                                                                                cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCa                                                        >  1:94252/1‑234 (MQ=33)
                                                                                                                                                                                                    gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACa                                                    <  2:94252/234‑1 (MQ=33)
                                                                                                                                                                                                        gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                                                                                                                >  2:93197/1‑106 (MQ=32)
                                                                                                                                                                                                        gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCCATAGCCTGATTTAGCGTGATTCTATAGGTCGGATAAGGc        >  2:410505/1‑274 (MQ=18)
                                                                                                                                                                                                             cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                                                                                      >  1:731601/1‑95 (MQ=33)
                                                                                                                                                                                                             cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                 >  2:27066/1‑100 (MQ=33)
                                                                                                                                                                                                             cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCATACGCCAGACAGCGCAATACCCTGCTTTAGCGTGATTTTGTAGGTCGGATACGGCGtttc   >  2:797053/1‑273 (MQ=16)
                                                                                                                                                                                                             cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAATTAGCGTGATTTTGTATGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACCCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACCCCAGACAGCGCAACAGCCTTCTTTCGCGTGATTTTCTAGGTCGGACAAGGCGTTTac  >  1:111956/1‑274 (MQ=11)
                                                                                                                                                                                                                       tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATc                                                                    >  1:208294/1‑199 (MQ=34)
                                                                                                                                                                                                                           ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                <  2:208294/199‑1 (MQ=34)
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                
AATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAT  >  NC_000913/4295789‑4296267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: