| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 417,391 | G→C | A84P (GCC→CCC) | phoB → | response regulator in two‑component regulatory system with PhoR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 417,391 | 0 | G | C | 98.3% | 169.1 / ‑6.1 | 60 | A84P (GCC→CCC) | phoB | response regulator in two‑component regulatory system with PhoR |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base C (32/27); total (33/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
CTGTGAATCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGA > NC_000913/417254‑417527 | ttgtgAATCAACAGAAGGAACCCTGGCCGGATTTAATGCACCACGACTGGATGTGACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCaga < 2:378039/142‑1 (MQ=255) gAATCGACTGCTTGGACCCTGGCCCGTTTTGATTCTCCTCGTCTGTATGTTACCTGGCGGCGCCGGTATCCAGTGCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAgggg < 1:472558/143‑1 (MQ=255) aaTCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGga > 2:862829/1‑143 (MQ=255) tctgcTGAATGCACCCTGGTCGGGTTCAATTCCGCTCTGCTGTGTCTGCTCCGCCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGGGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGgaag < 1:640711/139‑1 (MQ=255) tCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGCGGGgaag > 1:589275/1‑143 (MQ=255) aaCTGAGGGAACCCTGGCCGGCTTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGgaagaa < 1:95770/143‑1 (MQ=255) aaCTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGgaagaa < 2:448112/143‑1 (MQ=255) aaCTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGAGGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGgaagaa > 2:76715/1‑143 (MQ=255) aCTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACTCGGCGGCTCCGGTATCCAGTTCATCAAACACCTCACGCGCGAGACGATGACGCGGGATATTCCAGTGGTGATGTTGACGCCCGGAGGGcaaggag > 1:348226/1‑143 (MQ=255) aCTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGgaagaag < 1:457309/143‑1 (MQ=255) cTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATGCAGTTCATCAAAAACCTCAAGCGCGAGTCGATGACCCGGGATATTGCAGTGGTCGTGTTGACCGCCGCAGGGgaaggaga > 2:147845/1‑143 (MQ=255) tgaatgaaCCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGgaagaagaa < 2:815520/143‑1 (MQ=255) tgaatgaaCCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGgaagaagaa < 1:697992/143‑1 (MQ=255) gaaCCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGaagaagaagcacg > 1:765017/1‑139 (MQ=255) cTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTGATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTgc > 2:668900/1‑143 (MQ=255) cTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTgc > 2:340709/1‑143 (MQ=255) aaTTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGCCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGCCAGAAGAAGTGCGCGTGCGCGGCCTTGaaa > 2:325079/1‑143 (MQ=255) actcgccgtgACTGGAGGGTGCGTGGCGGCTCCGGTATCCAGTTCATCAAACCCCTCGAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAAc < 2:787068/134‑1 (MQ=255) gtatcttcGACTGGATGTTACCTGGCTGCTCCGGTCTACTAGCCATCAAACACCTCAAGCTCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAAcc < 1:181897/137‑1 (MQ=255) tcGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTTCAGTGGTGATGTTGACCCCCAGACGGGAGGCAGAAGATCGCGAGCGCGTCCTTAAACGCGGagcg > 1:30637/1‑143 (MQ=255) tcGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGcgcg > 2:34770/1‑143 (MQ=255) cGACTGAATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATCCCAGTGGTGAGGTTGACCCCAAGAGGGGAAGAAGAAGATCGCGTGCGGGGCCTTGAACCAGGCGAgg > 1:819286/1‑143 (MQ=255) aCTCGAGGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAGGCGCGAGTCGATGGCCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGAGCGCGTGCGCGGCCTTGAAACCGGCGCGGAt < 1:354796/143‑1 (MQ=255) gggAGGTTCCCGGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGa < 1:610259/142‑1 (MQ=255) ggcTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGAACCCCAGAGGGGAAGCAGAAGATCGCGTGAGCGGCCTTGAGACCGGCGAGGCTGACTGTATccccgaccc > 2:173411/1‑138 (MQ=255) tCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGCAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCACGGATGACTATATCACCAAGCCGtt > 1:367166/1‑143 (MQ=255) tCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATAACCACGctgcg > 2:590711/1‑139 (MQ=255) cGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGtttt > 1:647095/1‑143 (MQ=255) ccAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGa < 1:187776/143‑1 (MQ=255) ccAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGa > 2:187776/1‑143 (MQ=255) aGTTCAGCAGCCGCCTCAAGCGCGAGTCGATGACCCGGGATATCCCAGTGGTCAGGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAg < 1:688865/143‑1 (MQ=255) cacacacCTCCCGCGCGAGTCGCTGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGCGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCCAGCCGTTTTCGCCGAAGGAGCtgg < 2:127282/140‑1 (MQ=255) aaaCACCTCAAGCGCGAGTCGATGACACGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATGTCACCCAGCCGTGTGCGCAGCAGGAGCcggc > 1:837788/1‑142 (MQ=255) ccgcAAGCGCGAGTCGATGCCCCGGGATAGTCCAGTGGCGATGTGGACCCCCAGAGGGGAAGAAGAAGACCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGgcgc < 2:307794/140‑1 (MQ=255) aaGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGCGGAAGAAAAAGATAGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCTTTTTAGCCGAAAGAACTGGTGGCGCGAAt > 1:278050/1‑143 (MQ=255) gcgcGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGGATCa > 2:836486/1‑143 (MQ=255) gcgAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCaaa < 2:690888/143‑1 (MQ=255) gcgAGTCGATGACCCGGGATATCCCCGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCaaa < 2:363933/143‑1 (MQ=255) aGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGACGGAGCTGGTGGCGCGAATCAAAGCg > 1:65340/1‑143 (MQ=255) gTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCgg < 1:475920/143‑1 (MQ=255) gTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCgg > 2:752444/1‑143 (MQ=255) aTGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAAt < 2:716804/143‑1 (MQ=255) aTGACCCGGGAGATTCCAGTGGTGATGTGGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCGGGTGGCGGGAGTGAAAGCGGGaaa > 1:138226/1‑142 (MQ=255) cgcGGGATGTGCCGGTGGTGAGGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGCAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATgcgc < 1:275079/141‑1 (MQ=255) ggggATATGCCGGTGGTGATGTGGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTGTGCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCg < 1:353165/142‑1 (MQ=255) cGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGACACCGGCGCGGATGACTATATCACCAAGCCGTTTTCTCCGCGGGAGCGGGTGGCGCGAATCAAAGCGCTAATGCCCCg > 1:97814/1‑143 (MQ=255) cGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCg < 2:107412/143‑1 (MQ=255) cGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGAGGACTATATCACCAAGCCGTTTTCGCCGAGGGAGCTGGTGGCGCGACCCAAAGCGGTAATGCCCCg > 1:599560/1‑143 (MQ=255) cGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAACATCGCGTGCGCGGCCTTGAACCCGGCGCGGATGACTATATCACCAAGCCGCTTTCGCCGAAGGAGCTGGTGGCCAGAACCAAAACggcaccgccccg > 1:342189/1‑133 (MQ=255) attcgggtggtgATGTTGACCCCCAGGTGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTc < 1:256818/137‑1 (MQ=255) ttCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCg < 2:553227/143‑1 (MQ=255) ttCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCg < 2:781960/143‑1 (MQ=255) ttCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCGGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTca > 2:647384/1‑142 (MQ=255) tCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATGTCGc > 2:136869/1‑143 (MQ=255) cAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCa > 2:414951/1‑143 (MQ=255) gtgATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGGGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGCCTCACAGCGGTAATGCGCCGAATTTCGCCAACGGc > 1:572546/1‑143 (MQ=255) tgATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTACGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCg > 1:433688/1‑143 (MQ=255) aTGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGCAACCGGCGCGGATGACTATATCCCCAAGCCGGTCTCGCCGAAGGCGCGGGTGGCGCGAACAAAAGCGGCAACGCGCCGGAATTCGCCAACGGAggg > 1:659971/1‑142 (MQ=255) gTTGACCCCCAGAGGCGAGCAAGAAGATCGCGCGTGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTgg < 2:576256/143‑1 (MQ=255) ttGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATAACCAAGCCAAAATCGCAGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGa > 2:331580/1‑143 (MQ=255) | CTGTGAATCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGA > NC_000913/417254‑417527 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |