Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,509,477 C→T intergenic (‑53/‑151) glk ← / → yfeO glucokinase/putative ion channel protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,509,4770CT97.7% 125.8 / NA 44intergenic (‑53/‑151)glk/yfeOglucokinase/putative ion channel protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (23/20);  minor base A (1/0);  total (24/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCAT  >  NC_000913/2509338‑2509615
                                                                                                                                           |                                                                                                                                          
cTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAGCTGCTCCGCTTAAGTCACAATAATTCTTTCTCACACTGTAATTACTTgg                                                                                                                                         >  2:143460/1‑143 (MQ=255)
  gCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAGTACTTgggg                                                                                                                                       >  2:182614/1‑143 (MQ=255)
        tttCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATaa                                                                                                                                 <  2:190062/143‑1 (MQ=255)
        tttCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATaa                                                                                                                                 <  2:117778/143‑1 (MQ=255)
            accacTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTCAAACTGCTCCGCTAAAGTCAAACTAATTCTTTCTCACACTGTCCATACTTGGGGGAATaacaac                                                                                                                             >  1:868892/1‑143 (MQ=255)
              cgcTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATaacaacaa                                                                                                                           <  1:635339/141‑1 (MQ=255)
                 tGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGc                                                                                                                        >  2:396294/1‑143 (MQ=255)
                   gCAATATCACACAGAGCGAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCg                                                                                                                      <  1:874733/143‑1 (MQ=255)
                      atatCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGACATCACAACAACGCCCGaa                                                                                                                   >  2:651747/1‑143 (MQ=255)
                            cacaGGGCCAGCGGTGCGGTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATTATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAaggcag                                                                                                             <  2:482711/143‑1 (MQ=255)
                            cacaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAaggcag                                                                                                             <  2:387459/143‑1 (MQ=255)
                             acaGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAaggcagg                                                                                                            >  1:287342/1‑143 (MQ=255)
                                      gACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTa                                                                                                   <  1:428536/143‑1 (MQ=255)
                                          tGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGAATAAAAACAACGCGGGAAAGGCAGGCTACGTGTAAata                                                                                               >  2:2190/1‑143 (MQ=255)
                                           gCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAatat                                                                                              <  2:275132/143‑1 (MQ=255)
                                                   gccgccCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTgg                                                                                      <  2:345849/143‑1 (MQ=255)
                                                       cccACATCACCGACTAATGCATACTATGAAATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCCAGCTCCCTTTAATGATCGAACTGGGtgc                                                                                  >  1:64686/1‑141 (MQ=255)
                                                             tCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAACACTGGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAAt                                                                            >  1:659951/1‑143 (MQ=255)
                                                                 cgtcTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACt                                                                        <  2:579119/140‑1 (MQ=255)
                                                                  gACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACtt                                                                       >  2:253377/1‑143 (MQ=255)
                                                                     tAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAAAATCGATCTGGGTAACACAATAACTTTAt                                                                    >  2:437724/1‑143 (MQ=255)
                                                                         gCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACATGAGGCCATAACAACACCGCCGGAAATGCAGGCTCCCTGTAAATATCGATCCGGGTCACACAATCGCTTTATCgct                                                                >  1:476428/1‑141 (MQ=255)
                                                                                  gTCATTCTTCAACAGCACCGCTAAAGGCAAAATAATTCCGTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATACCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCa                                                       <  2:42201/143‑1 (MQ=255)
                                                                                     aTTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACCACGCCGGAAAGGCAGGCTCCCTGTACATATCGATCTCGGTCCCCCACTTACTTTATCGTTTCAGCACCACtt                                                    >  2:582725/1‑143 (MQ=255)
                                                                                     aTTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAAtc                                                    >  2:464964/1‑142 (MQ=255)
                                                                                      ttcttcAACTACTCAGCTAAAGTCAAAATAGTTCTTTCTCACACTGTAAATACTTGGAGACGTAAAAACAACGCCGGAGAGGCAGGCTCCCTGTAAAAATCGATCGGGGGCACACAATTACTTTATCGTTTCAGCACCAGTTg                                                   >  1:504476/1‑143 (MQ=255)
                                                                                         ttcAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTCACTACTTGGGGGCATAACAACAACGCCGGAAAGGCAAGCTCCCTGTACATATCGATCTGCGTCACAACACTCCGTTGTCGTTGTAGCACCAATGGCAg                                                >  1:805734/1‑143 (MQ=255)
                                                                                             aCTGCTCCGCTAAAGTCAAAATAATTCTTTCTCTCACTGTAAATTTTTGGGGTGCGCACAACAACGGCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGAt                                            <  2:199942/143‑1 (MQ=255)
                                                                                               tGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGc                                          <  2:256703/143‑1 (MQ=255)
                                                                                               tGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAACACTTCGTGGCATAACACCACCACCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGc                                          <  1:8944/143‑1 (MQ=255)
                                                                                                 cTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTCTCGTCTCAGCACCACTTGCAGCGAGGCCt                                        >  1:278320/1‑143 (MQ=255)
                                                                                                 cTCCGCTAAAGTCAAAATAAGTCTTGCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGATAGGCAGGCTCCCTGTAAATATCGATCTAGGTCACACAATACCTTTATCGTTTCAGCACCAATTGCAGCAATGCCt                                        >  1:439116/1‑143 (MQ=255)
                                                                                                  tCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGACAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTCTATCGTTTCCGTACAAATTGCACAGATGCCtt                                       >  1:217671/1‑143 (MQ=255)
                                                                                                           gTCAAAATAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGc                              >  2:604246/1‑143 (MQ=255)
                                                                                                              aaaaTAATTCTTTCTCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTgg                           >  2:751618/1‑143 (MQ=255)
                                                                                                                   aatgcgcacgcacaCTGTAAATGCTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCaaa                      <  2:361027/133‑1 (MQ=255)
                                                                                                                       ctttctCACTCTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAACTATCGATCTTGGTCCCCCACTTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTaa                  <  2:877900/143‑1 (MQ=255)
                                                                                                                       ctttctCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTaa                  <  2:148766/143‑1 (MQ=255)
                                                                                                                         ttctCACACTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGTTCACACAATTACTTTCTCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGt                <  2:287342/143‑1 (MQ=255)
                                                                                                                             cacatcgTAAAGACTTGGGGGCATAACAACGACGCCGTAAATGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCt            <  2:684360/137‑1 (MQ=255)
                                                                                                                                 cTGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGAcc        <  2:863320/143‑1 (MQ=255)
                                                                                                                                  tGTAAATACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCGCTGTAAATATCGATCTGGGTCACACCATTACTTTATCGTTTCAGCACCACTTGAAGCGATGCCTTTTTGCACCCTGGGCAATCTAAGTATCTGAccc       >  1:111419/1‑143 (MQ=255)
                                                                                                                                    tAAATACTTGGGGGCATAACAACAACGCCGGAAAGGAAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCg     >  2:570167/1‑143 (MQ=255)
                                                                                                                                       aTACTTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATCACTATATCGGTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAACGCAAGTATCTCACCCCTCag  >  2:715859/1‑142 (MQ=255)
                                                                                                                                           |                                                                                                                                          
CTGCGAGATTTCACCACTGGCAATATCACACAGAGCAAGACGTGCGTTGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCAT  >  NC_000913/2509338‑2509615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: