| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,726,507 | Δ1 bp | coding (485/600 nt) | nemR → | transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,726,507 | 0 | T | . | 96.0% | 95.0 / ‑3.1 | 25 | coding (485/600 nt) | nemR | transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (11/13); minor base A (1/0); total (12/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGAC > NC_000913/1726371‑1726644 | gATCTGGCAGAAGAAAAGCGACGCGCGGTGGATAAAGGGGGTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTAc < 2:238406/143‑1 (MQ=255) agaagaTATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATGGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACtgtc > 2:482147/1‑142 (MQ=255) gcgcAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCGCTGCTCTCTCAGGCGCGGGAAAAGGGCCGTGAGAACCATAGATTAACCTTTTGTGGCGAACCGCTGCAACAGACGCAAG‑GCATTACGCACTGTGTCTTGgagc > 1:1729/1‑143 (MQ=255) cgcAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGcgcg > 1:124327/1‑143 (MQ=255) gATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGcc > 2:58481/1‑143 (MQ=255) tAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCaa > 1:430680/1‑143 (MQ=255) gcggcgTGATCGCCCTGTTCTCTCCGGCGCTGGAAAATGGCCGGGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCa < 1:281632/143‑1 (MQ=255) ctctctCAGGCGCTGGAAAATGGCCGTGAGAACCATTGCTCCACCTATCGTAGCGAACCTCTGCAACAGACACAA‑AGCTCTCCCCACTGTCGCTTGACGTGAATCTGCAGTCCCAAATTTCACGCAGATTctacaaccgtgaa > 1:367466/1‑131 (MQ=255) gcgcTGGAACATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTg < 2:429397/143‑1 (MQ=255) tGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGccc > 1:489643/1‑143 (MQ=255) aaaTGGCCGTGAGAACCATTGTTGAACCTTTTGTGGCGAACCGCGGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGt < 2:315989/143‑1 (MQ=255) ccGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCCCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTaaaaaa > 2:306530/1‑143 (MQ=255) cagTGAGAACCATGGGTAAACCATTTGGGGCGAACCGATGCAACAGGCAGAAG‑GCTTTACGGACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGAAGTTTCGAGCCACTGGAAAACGCGCTGGAACATGTaaaaaa < 1:131413/141‑1 (MQ=255) cATTGTTTGGCGTATAGTGTCGGACGGCTCCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCg < 2:137566/143‑1 (MQ=255) aaCCTTTTGTGGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCACGAATCTGCAGGCCCAAATTTCGCGCAGTTTCGAGCCACTGGAAACCGCGCTGGCCCATGTAACAAACATTATTCCGACGCCCGc > 1:498400/1‑143 (MQ=255) tgggggCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTa < 1:17508/139‑1 (MQ=255) tgtgGCGAACCGCTGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCCCAGTTTCGAGCCACTGCAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAg < 2:517017/143‑1 (MQ=255) ggCGAACCGCGGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTGGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAg < 1:120674/143‑1 (MQ=255) cGCTGCAACAGCCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCAtttt < 2:375238/143‑1 (MQ=255) tcgacAACATGCACACG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCAttttt < 1:408896/139‑1 (MQ=255) tGCAACAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTa < 2:457274/143‑1 (MQ=255) aCAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACGGGAAAACGCGCTGGCCCATGTAAAAGACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTGTCTCAc > 2:52795/1‑143 (MQ=255) aCAGGCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGATTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCAc < 2:261727/143‑1 (MQ=255) ggCACAAG‑GCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTACACGCGCTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTGTCACCAg > 1:86153/1‑143 (MQ=255) cacaAG‑GCTTTACGCACTGTGGCTTGGCGCGACTCTACAGGCCAAAATTTCACGCACTTTCAAGCCACTACAAAATGCACTGGAGCATGTATAAAACATAATTACAACGCCAGGCGTCTAGCAATCATTAATTGTAACCAgcg > 1:272652/1‑141 (MQ=255) | GATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGAC > NC_000913/1726371‑1726644 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |