Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,977,594 +C coding (998/1032 nt) galR → galactose‑inducible d‑galactose regulon transcriptional repressor, autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,977,5931.C90.9% 53.1 / 0.5 23coding (997/1032 nt)galRgalactose‑inducible d‑galactose regulon transcriptional repressor, autorepressor
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (13/7);  total (15/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

CCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCG  >  NC_000913/2977453‑2977729
                                                                                                                                              |                                                                                                                                         
cttccgtttgTTACCTTATTGTGATGATGGCGACGCAGGCGGCCGAACTGGCGTAGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTcc                                                                                                                                         <  2:309557/134‑2 (MQ=255)
ccaccGTGCGGGACCCAACCGGGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCCCTCCCATAAATCGCTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTcc                                                                                                                                         <  2:120398/143‑2 (MQ=255)
    cGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTCAATCCGACGCTTGTACGTCGTCATTCAGTGTCAACTCCGTccgctg                                                                                                                                     <  2:269700/143‑6 (MQ=255)
           gcaccAATCGTGACGATGGCTACCCAGTCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCGTTAGTCCGACGCTTGTACGTCGTCATTCAGTGTCAACTCCGTCCGCTGGAGGCaa                                                                                                                              <  2:519082/140‑1 (MQ=255)
            aCCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCCGCTGGAGGCAAg                                                                                                                             <  2:324812/143‑1 (MQ=255)
                       aCGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCCGCTGGAGGCAAGTGTTCGTGCat                                                                                                                  >  2:377514/1‑142 (MQ=255)
                       aCGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCCGCTGGAGGCAAGTCATCATCCaa                                                                                                                  >  1:348561/1‑143 (MQ=255)
                       aCGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGAGAATCGCCCTCTCCCGGAAATCACTAATGGCTTTAGTCCGACGCTGGTACGTCGTCAGTCAGTGTCAACTCCGTCCGCCGGCGGCAAGTAATCATGCaa                                                                                                                  >  1:414153/1‑143 (MQ=255)
                            ggCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAAACACTAATGTCTTTAGTCCGACGCTGGTACGTCGGCATTCAGTGCAAACGCCGTACGCTGGAGGCAAGCCATCACACAAcaaac                                                                                                             >  1:355044/1‑139 (MQ=255)
                              cGACGCCGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTGCGTCGTCATTCAGTGTCAACTCCGTCCGCTGGAGGCAAGTCATCATGCAACCAGCGa                                                                                                           <  1:379231/143‑1 (MQ=255)
                                     ggCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTCATGTCTTTAGTCCGACGCTGGTACGTCGTCAGTCAGTGTCAACTCCGTCCGCTGGAGGCAAGTCATCATGCAACCACCGCCGAACCg                                                                                                    >  1:147276/1‑143 (MQ=255)
                                        tGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAg                                                                                                 <  1:268613/143‑1 (MQ=255)
                                             accttgCTTTGGTGCGGGCGGATAGTCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCCGCTGGAGGCAAGTCTTCATGCAACCAGCGACTAACCGCAGTTaaa                                                                                            <  1:180847/138‑1 (MQ=255)
                                                          gcTGGCGGACCTTGGCGCTCGCCCGGCGGTCACTAATGTCTTTCGTCCGACGCGGGTACGTCGTCATTGAGCGTCCACTCCGTCCGCTGGATGAAAGTCATCATGTAACCAGCGAGTAACCGCAGTTAAAGCAATTCCAgcgc                                                                               <  1:223365/143‑1 (MQ=255)
                                                                            tctcCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCCGTAATTCTTCGATGGt                                                             >  2:431449/1‑143 (MQ=255)
                                                                                                  tttAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGGAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACcgc                                       >  2:383147/1‑143 (MQ=255)
                                                                                                       tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTg                                  >  2:298667/1‑143 (MQ=255)
                                                                                                       tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGCCGACTCAACCGCGcccg                                  >  1:411653/1‑141 (MQ=255)
                                                                                                         cGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGGTGGTCTGGCGACGGCGAATCAACCGCGCCTgcc                                >  2:73631/1‑141 (MQ=255)
                                                                                                           aCGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCCGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCa                              >  2:129595/1‑143 (MQ=255)
                                                                                                              cTGGTACGTGGTCATTGAGTGCCAACTCAGTCTGCGGCAGATAAGTCGTCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTa                           <  2:468474/143‑1 (MQ=255)
                                                                                                                            ttCAGTGTCAACTCCGTCCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTc             >  2:289676/1‑143 (MQ=255)
                                                                                                                             gccgggtCCACTCCGTCCGCGGGAGGCAAGTCAGCATGCAACCAGCGACTAACCGCGGTTAAAGCAAGTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCt            <  1:429979/138‑1 (MQ=255)
                                                                                                                                   tCAACTCCGACCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAACTCTACGATGGTCTGGCGACGGCGAATCCACCGCGCCTGAGCATGACCAACCAGAAGCTCTGGTCAc      >  1:284883/1‑143 (MQ=255)
                                                                                                                                   tCAACTCCGTCCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGACTCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAAc      >  2:233569/1‑143 (MQ=255)
                                                                                                                                       cTCCGTCCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGTACTTCTGGTAACAGCg  >  2:493704/1‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                         
CCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCG  >  NC_000913/2977453‑2977729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: