| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,977,594 | +C | coding (998/1032 nt) | galR → | galactose‑inducible d‑galactose regulon transcriptional repressor, autorepressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,977,593 | 1 | . | C | 90.9% | 53.1 / 0.5 | 23 | coding (997/1032 nt) | galR | galactose‑inducible d‑galactose regulon transcriptional repressor, autorepressor |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (13/7); total (15/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
CCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCG‑T‑C‑GCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCG > NC_000913/2977453‑2977729 | cttccgtttgTTACCTTATTGTGATGATGGCGACGCAGGCGGCCGAACTGGCGTAGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCG‑T‑cc < 2:309557/134‑2 (MQ=255)ccaccGTGCGGGACCCAACCGGGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCCCTCCCATAAATCGCTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCG‑T‑cc < 2:120398/143‑2 (MQ=255) cGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTCAATCCGACGCTTGTACGTCGTCATTCAGTGTCAACTCCG‑T‑ccgctg < 2:269700/143‑6 (MQ=255) gcaccAATCGTGACGATGGCTACCCAGTCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCGTTAGTCCGACGCTTGTACGTCGTCATTCAGTGTCAACTCCG‑TCC‑GCTGGAGGCaa < 2:519082/140‑1 (MQ=255) aCCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCG‑TCC‑GCTGGAGGCAAg < 2:324812/143‑1 (MQ=255) aCGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCG‑TCC‑GCTGGAGGCAAGTGTTCGTGCat > 2:377514/1‑142 (MQ=255) aCGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCG‑TCC‑GCTGGAGGCAAGTCATCATCCaa > 1:348561/1‑143 (MQ=255) aCGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGAGAATCGCCCTCTCCCGGAAATCACTAATGGCTTTAGTCCGACGCTGGTACGTCGTCAGTCAGTGTCAACTCCG‑TCC‑GCCGGCGGCAAGTAATCATGCaa > 1:414153/1‑143 (MQ=255) ggCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAAACACTAATGTCTTTAGTCCGACGCTGGTACGTCGGCATTCAGTGCAAACGCCG‑TAC‑GCTGGAGGCAAGCCATCACACAAcaaac > 1:355044/1‑139 (MQ=255) cGACGCCGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTGCGTCGTCATTCAGTGTCAACTCCG‑TCC‑GCTGGAGGCAAGTCATCATGCAACCAGCGa < 1:379231/143‑1 (MQ=255) ggCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTCATGTCTTTAGTCCGACGCTGGTACGTCGTCAGTCAGTGTCAACTCCG‑TCC‑GCTGGAGGCAAGTCATCATGCAACCACCGCCGAACCg > 1:147276/1‑143 (MQ=255) tGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCG‑TCC‑GCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAg < 1:268613/143‑1 (MQ=255) accttgCTTTGGTGCGGGCGGATAGTCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCG‑TCC‑GCTGGAGGCAAGTCTTCATGCAACCAGCGACTAACCGCAGTTaaa < 1:180847/138‑1 (MQ=255) gcTGGCGGACCTTGGCGCTCGCCCGGCGGTCACTAATGTCTTTCGTCCGACGCGGGTACGTCGTCATTGAGCGTCCACTCCG‑TCC‑GCTGGATGAAAGTCATCATGTAACCAGCGAGTAACCGCAGTTAAAGCAATTCCAgcgc < 1:223365/143‑1 (MQ=255) tctcCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCG‑TCC‑GCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCCGTAATTCTTCGATGGt > 2:431449/1‑143 (MQ=255) tttAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCG‑TCC‑GCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGGAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACcgc > 2:383147/1‑143 (MQ=255) tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCG‑TCC‑GCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTg > 2:298667/1‑143 (MQ=255) tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCG‑TCC‑GCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGCCGACTCAACCGCGcccg > 1:411653/1‑141 (MQ=255) cGACGCTGGTACGTCGTCATTCAGTGTCAACTCCG‑TCC‑GCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGGTGGTCTGGCGACGGCGAATCAACCGCGCCTgcc > 2:73631/1‑141 (MQ=255) aCGCTGGTACGTCGTCATTCAGTGTCAACTCCG‑TCC‑GCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCCGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCa > 2:129595/1‑143 (MQ=255) cTGGTACGTGGTCATTGAGTGCCAACTCAG‑T‑CTGCGGCAGATAAGTCGTCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTa < 2:468474/143‑1 (MQ=255) ttCAGTGTCAACTCCG‑TCC‑GCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTc > 2:289676/1‑143 (MQ=255) gccgggtCCACTCCG‑TCC‑GCGGGAGGCAAGTCAGCATGCAACCAGCGACTAACCGCGGTTAAAGCAAGTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCt < 1:429979/138‑1 (MQ=255) tCAACTCCGAC‑C‑GCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAACTCTACGATGGTCTGGCGACGGCGAATCCACCGCGCCTGAGCATGACCAACCAGAAGCTCTGGTCAc > 1:284883/1‑143 (MQ=255) tCAACTCCG‑TCC‑GCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGACTCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAAc > 2:233569/1‑143 (MQ=255) cTCCG‑TCC‑GCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGTACTTCTGGTAACAGCg > 2:493704/1‑143 (MQ=255) | CCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCG‑T‑C‑GCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCG > NC_000913/2977453‑2977729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |