Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1978503	1979278	776	30 [0]	[0] 30	insB1–insA	insB1, insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1978502	0 (0.000)	29 (1.030)	25/268	0.1	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

CTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATG  >  NC_000913/1978365‑1978640                                                                                                                                          |                                                                                                                                           cTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATCATGCGTGATGCAGATCACACAAAACACTCAATTCCTTAACATAAATGtataa                                                                                                                                       <  1:244082‑M1/143‑6 (MQ=255)            tCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGTGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactttt                                                                                                                            <  1:70365‑M1/143‑17 (MQ=255)            gctAAGATTTTGCCCAAATCGACGCGACGGCACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactttt                                                                                                                            <  1:209985‑M1/142‑17 (MQ=255)              tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGGGGGGGACTGCCATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCACTTACTTCACATAAATGtataagccataccatcgc                                                                                                                          >  1:442196‑M1/1‑125 (MQ=255)                 tatTTTTCCTGAGTCGGCGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtttt                                                                                                                       <  1:261510‑M1/142‑22 (MQ=255)                   tttttCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                     <  2:81979‑M1/143‑24 (MQ=255)                          tAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGTTCACACAAAACTCCCAATTACTTAGCATAAATAtataagtcatacgtttgttttgggtgtatt                                                                                                              >  2:241524‑M1/1‑113 (MQ=255)                            aaTCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactttagttttgggtgtatttc                                                                                                            >  1:441404‑M1/1‑111 (MQ=255)                                   gCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgt                                                                                                     <  1:445077‑M1/143‑40 (MQ=255)                                       cTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaacctgaacaa                                                                                                 >  2:321164‑M1/1‑100 (MQ=255)                                                 cACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAGTGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactcttgttttgggtgtatttcaatctgttaaaaagtttttcg                                                                                       >  1:69945‑M1/1‑90 (MQ=255)                                                   cTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgct                                                                                     >  1:286544‑M1/1‑88 (MQ=255)                                                   cTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgct                                                                                     >  1:286032‑M1/1‑88 (MQ=255)                                                             aTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaa                                                                           >  2:310626‑M1/1‑78 (MQ=255)                                                                         ggggCTTCGCTTTCAATTAATGCGTGATGCATATCACACAAAACACTAAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaa                                                               <  1:262235‑M1/143‑78 (MQ=255)                                                                         ggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaa                                                               <  2:31897‑M1/143‑78 (MQ=255)                                                                                 gATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtatcagtcatacttttgttttgggtgtatttcaatctgtgaaaaagttttttgcttcgctaggagacacaaatgagaccgaaaga                                                       >  1:279788‑M1/1‑58 (MQ=255)                                                                                   ttttCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataat                                                     >  1:454082‑M1/1‑56 (MQ=255)                                                                                            atGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagcttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaacggg                                            >  2:439709‑M1/1‑47 (MQ=255)                                                                                               cGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatg                                         >  2:318042‑M1/1‑44 (MQ=255)                                                                                                      cAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaatattcgaaatgggacgttttgca                                  >  1:177363‑M1/1‑37 (MQ=255)                                                                                                      cAGATCACACAAAACACTCAATTACTTAACACAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgca                                  <  1:138130‑M1/143‑107 (MQ=255)                                                                                                        gATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgcttttggtgtatttaaatcttttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggctgttgcccaca                                >  1:372824‑M1/1‑35 (MQ=255)                                                                                                          tCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagt                              <  2:286544‑M1/143‑111 (MQ=255)                                                                                                          tCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagt                              <  2:286032‑M1/143‑111 (MQ=255)                                                                                                              acaAAACACTCAATTACCTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcg                                                   <  1:488932‑M1/118‑90 (MQ=255)                                                                                                              acaAAACACTCAATTACCTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcg                                                   >  2:488932‑M1/1‑29 (MQ=255)                                                                                                                aaaaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaactgggatgttgcgcaccgtcaaaat                        >  2:479667‑M1/1‑27 (MQ=255)                                                                                                                                 aaCATAAATGtataagtcagacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataat       <  2:81553‑M1/143‑134 (MQ=255)                                                                                                                                      aaaTGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatct  <  1:383870‑M1/143‑139 (MQ=255)                                                                                                                                          |                                                                                                                                           CTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATG  >  NC_000913/1978365‑1978640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑