Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,542 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5420CA96.9% 82.3 / NA 32P1100Q (CCG→CAG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (14/17);  total (15/17)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA  >  NC_000913/4184406‑4184680
                                                                                                                                        |                                                                                                                                          
gCGGTTAAACGCCGTATCCAGCCTGGTGACAAGACAGCAGGTCGTCACGGTAACCCGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTCCGATGAAAACGGACCGCCGGTAGACACCCTACTGCACCAGccgcg                                                                                                                                      >  1:460658/1‑139 (MQ=255)
 cgcttAAACGCCGTATCCATCCTGGTGACAAAATGGCAGGTCGTCACACTAACACGGGTGTAATTTCTAAGATCAACCCGCTCGAAGATATGCCTTACGATGAAAACGGTACGCCTTTAGACAGCGTACTGAACCAGCTGGGc                                                                                                                                     <  2:318635/140‑1 (MQ=255)
 cGGTTAAACGCCGTATCCAGGCGGTTGACAAGATGGCATGTCGTCACGGTAAGAAGGGTGCAATTTCTAAGAGCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGc                                                                                                                                     <  2:291953/143‑1 (MQ=255)
    ttAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATCAAAACGGAACACCGGTAGACATCGTACTGAACCAGCTGGGCGTa                                                                                                                                  >  1:310793/1‑143 (MQ=255)
     gaaaCGCCGTATCCAGCCTGGTGGCAGGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAATCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAc                                                                                                                                 <  1:505266/142‑1 (MQ=255)
          gCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGACCCCGCCGGGCGTACCGcct                                                                                                                            >  1:447465/1‑143 (MQ=255)
           ccGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGCGTAATTTCTAAGATCAACCCGATCGCCGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACCGAACCAGCTGGGCGTACCGtctc                                                                                                                           >  2:150718/1‑143 (MQ=255)
                 ccgcccTGGTGACAATATGGCCGGTCTTCACGGTAACATGGGTGTAATTTCTAAGATCAACCCGATCGAAGAGATGCCTGACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGa                                                                                                                     <  1:444579/139‑1 (MQ=255)
                  aagtggggcgACAAGATAGCAAGTGCTAACGGTAAGAAGTGTGTAATGTCTAAGATCAACCGGATCGAAGATATGCCTTACGAGGAAAGCGGTACGCCGGTAGACAGCTTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa                                                                                                                    <  1:379668/134‑1 (MQ=255)
                                 aTGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCt                                                                                                     >  1:355325/1‑143 (MQ=255)
                                      aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCGTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                                <  2:482912/143‑1 (MQ=255)
                                       ggTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTTGTATGAACATCGGTCAGATCCTCGAAAc                                                                                               <  2:100473/143‑1 (MQ=255)
                                               cGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCGCGTATGAACAACGGTCCGATCCGCGCCAccccgccgg                                                                                       >  1:224956/1‑137 (MQ=255)
                                               agtaaaCAAGGGGGTAACTGCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGGACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                       <  1:66250/139‑1 (MQ=255)
                                                  tAACAAGGGGGCAATTTCTAAGACCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTCAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTa                                                                                    <  2:255303/143‑1 (MQ=255)
                                                      aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGCCAGATCGTCGAAACCCACCTGGGTGTGGc                                                                                >  1:235848/1‑143 (MQ=255)
                                                         ggTGTAATTCTAAAAACCAACCCGATCGAAGAGATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGc                                                                             <  2:323387/143‑1 (MQ=255)
                                                          gtgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGCCAGATCCTCGCATCCCACCTGGGTATGGCTGCg                                                                            >  2:468059/1‑143 (MQ=255)
                                                           tgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGa                                                                           >  2:370648/1‑143 (MQ=255)
                                                              aaTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGAACCGTCTCGTATGAACATCGGTCAGATACTCGAAACCCGCCTGGGTGTGGCTGCGACAg                                                                        >  1:256200/1‑143 (MQ=255)
                                                                             cccGATCGAAGATATGCCTTACGCTGAACACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGa                                                         <  2:354099/143‑1 (MQ=255)
                                                                              ccGATCGAAGAGATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAt                                                        <  2:34815/143‑1 (MQ=255)
                                                                                 aTCGAAGATATGCCTGACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCaa                                                     <  1:296641/143‑1 (MQ=255)
                                                                                          atGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCt                                            >  1:375963/1‑143 (MQ=255)
                                                                                                    aTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGCACAAGCAGCAAAACGTAGCGGCGACAAGATCAACGCCATGCTGAAAcagcag                                  <  2:99314/143‑1 (MQ=255)
                                                                                                               aCGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACACGATCCACGCCATGCTGCAACAGCAGCAAGCAGCcgc                       >  2:146047/1‑143 (MQ=255)
                                                                                                                cGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCCCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTCCGACAGGCATCGCCGACACGACCCACGCCACGCTGCAACACCACCACGAAGTcgcc                      >  1:10403/1‑142 (MQ=255)
                                                                                                                    ggTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCCGAATGAAGTCGCGAAAc                  >  2:466451/1‑143 (MQ=255)
                                                                                                                     gTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCGAGAAGTCGCGAAACt                 >  2:15164/1‑143 (MQ=255)
                                                                                                                             cGTACCGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGGCGCGAAACTGCGCGAAt         <  2:485998/143‑1 (MQ=255)
                                                                                                                                accgagcccGCTGGGCGTACCGTCCCGTACGACCACCGGTCAGATCCGCGAAACCCACCCGGGGATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGACACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCa      <  1:477789/137‑1 (MQ=255)
                                                                                                                                 cttaaCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCAt     <  2:377883/140‑1 (MQ=255)
                                                                                                                                    aaCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCAGCGAAACCCCCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACCGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCa  <  2:250272/143‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                          
GCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA  >  NC_000913/4184406‑4184680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑