Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	417,391	G→C	A84P (GCC→CCC)	phoB →	response regulator in two‑component regulatory system with PhoR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	417,391	0	G	C	100.0%	63.2 / NA	25	A84P (GCC→CCC)	phoB	response regulator in two‑component regulatory system with PhoR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (15/10);  total (15/10)

TGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAA  >  NC_000913/417266‑417528                                                                                                                              |                                                                                                                                          tgaatgaaCCCTGGCCGGATTTAAGTCTCCTCGACTGGATGTGACCGGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGgaagaagaa                                                                                                                          <  1:434095/143‑1 (MQ=255) taaatgaaCCGTTGCCGGATTTAATTATCCTTGATTGCATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCAGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGgaagaagaa                                                                                                                          <  1:146145/141‑1 (MQ=255)    atgaaCCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGAt                                                                                                                       <  2:123247/143‑1 (MQ=255)        acgcttgCCCGATCTACTTCTGCGCGACTCGGTGCGACCTGGCGGCTCCGGGATCCGGTTCATCAAACTCCTGAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATcgcg                                                                                                                   <  1:137751/137‑1 (MQ=255)        aCCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGGCCCGGGATATTCCAGTGGCGAGGTTGACCCCCAGGAGGGAAGAAGACGATcgcg                                                                                                                   >  1:27858/1‑143 (MQ=255)                    tttAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTg                                                                                                       >  2:313287/1‑143 (MQ=255)                            tcctcGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGCAACCGgc                                                                                               >  2:81274/1‑143 (MQ=255)                             cctcGACTGGATGTTACCTGGCGGCTCCTGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGgcg                                                                                              <  1:205538/143‑1 (MQ=255)                              ctcGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCGGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGgcgc                                                                                             <  1:322876/143‑1 (MQ=255)                               tcGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTACATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTACGCGGCCTTGCAACCgccccc                                                                                            >  1:450925/1‑138 (MQ=255)                                             ccTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTAGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCAc                                                                              <  2:274213/143‑1 (MQ=255)                                                               cAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGCTGACTATATCACCAAGCCGTTTTCGCCGaa                                                            >  2:178356/1‑143 (MQ=255)                                                                      tcaAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATAACCAAGCCGTTTTCGCCGCAGGAGcgg                                                     >  2:411579/1‑141 (MQ=255)                                                                        aaaCACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGCTGCCTATATCACCAAGCCGTTTTCGCCGTACGCGCtggt                                                   >  2:164685/1‑143 (MQ=255)                                                                          cgtgcccgggggcgcGTCGAGGCGCCGGGATAGGCCAGTCGTGAGGTGGACCCCTGGAGGGGAAGTAGCAGATCGGGGGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCtggtgg                                                 <  1:242557/132‑1 (MQ=255)                                                                                       gAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGc                                    >  2:186447/1‑143 (MQ=255)                                                                                       gAGTCGATGACCCGGGATATTCCAGTGAAGACGATGACCCCCAGAGGGGAAGAAGAACATCGCGAGCGCGGCCTGGAAACCAGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGc                                    >  1:63454/1‑143 (MQ=255)                                                                                             aTGACGCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGTCTTGAAACCGGCGCGGATGACTATCTCACCAAGCCGTTTTCGCCGAAGGAGCGGGTGGCGCGAATCAAAGCGGTAAt                              >  1:90094/1‑143 (MQ=255)                                                                                             aTGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGGAGGAGCTGGTGGCGCGAATCAAAGCGGTAAt                              >  2:294348/1‑143 (MQ=255)                                                                                             aTGACCCGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAAt                              >  2:292185/1‑143 (MQ=255)                                                                                                   cGGGATATTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTGGGCGAAGGAGCAGGTGGCGCGAATGAAAGCGGTAATGCGCCg                        >  2:215976/1‑143 (MQ=255)                                                                                                        tatTCCAGTGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGACTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTAttt                   >  2:472277/1‑143 (MQ=255)                                                                                                          ttCCAGGGGTGATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTAAATAAATCTTCTGGGGCTTCTAATCAAAGCGGTAATGCGCCGTATTTCg                 <  2:99296/143‑1 (MQ=255)                                                                                                                   tgATGTTGACCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCGAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCGAAGCGGTACTGCGCCGTATTTCGGCCAGGGCg        >  1:388323/1‑143 (MQ=255)                                                                                                                         tcaCCCCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACCATATCACCAAGCCGTTGTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGaa  <  2:211815/141‑1 (MQ=255)                                                                                                                              |                                                                                                                                          TGAATGAACCCTGGCCGGATTTAATTCTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAA  >  NC_000913/417266‑417528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑