Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 702,066 G→C P229R (CCT→CGT)  nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913702,0660GC100.0% 53.4 / NA 18P229R (CCT→CGT) nagAN‑acetylglucosamine‑6‑phosphate deacetylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (11/7);  total (11/7)

TGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCA  >  NC_000913/701929‑702201
                                                                                                                                         |                                                                                                                                       
tGGCGGCAGTAACCAGACACAGTTGGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCa                                                                                                                                    <  1:85338/143‑1 (MQ=255)
                 cagagttgctcgagtttCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACgg                                                                                                                   <  1:63264/129‑1 (MQ=255)
                          tCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTACTCACCATGCAGGCCATCAGCAATAATACCGCAATAAATGTCCGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGCCCGGTAATATACGGCCTCGCGta                                                                                                          >  1:351554/1‑142 (MQ=255)
                            gcgtgtCAGCCGTGTAGCGTTGCGAATGTTGGCGCAATCAACATGCAGGCCATCAGCAATAATACCGCAATCAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGt                                                                                                        <  2:189220/138‑1 (MQ=255)
                                     aCGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTAGCGACCGGTAATATACGGCATCGCGGTGTGCAGCTggg                                                                                               >  2:27772/1‑143 (MQ=255)
                                     aCGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTACACGACCGGTAATATACGGCATCGAGTTGTAGAGAgggg                                                                                               >  2:77435/1‑143 (MQ=255)
                                      cGTTGATCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGCTCGCGCCCGCCAGGCTACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGt                                                                                              <  1:326656/143‑1 (MQ=255)
                                                 gCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTaa                                                                                   <  1:502325/143‑1 (MQ=255)
                                                  cGAATGTTGGCTTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAAt                                                                                  <  2:236556/143‑1 (MQ=255)
                                                                    aCATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGGTGGGTGGCCAAGGTAATCCCCGCGCGGAAACCGGc                                                                >  2:70261/1‑143 (MQ=255)
                                                                         cAGGCCATCAGCAATAATACCGCAATAAAGGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCAAGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAAGCCCCGCGCGGGAACCGGCTtgtg                                                           >  2:121677/1‑143 (MQ=255)
                                                                            gCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGCAACCggagagtgatt                                                        >  1:484899/1‑134 (MQ=255)
                                                                                 cAGCAACAATACCGCAATAAATGTCAGCTTCGTCGAGGTTCGCGCCCGCCAGGCCACGTTCACGACCGGTAAGATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTc                                                   <  2:443728/143‑1 (MQ=255)
                                                                                             cGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTCCAGCTGGGTGGCAACGGCACTCCCCGCGCGGAAACCGGCTTCTGCTGCTTTCACCGTCGCGttc                                       >  1:297040/1‑142 (MQ=255)
                                                                                                      tGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCg                              >  2:489279/1‑143 (MQ=255)
                                                                                                        tCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGATGTACAGATGGGTGGTAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCCTCTTGCAACGGCGCGGTGGAGGGAcacgc                            >  2:506964/1‑139 (MQ=255)
                                                                                                             ttCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGaaa                       >  2:156070/1‑143 (MQ=255)
                                                                                                                        tCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCCACCTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCg            >  1:375945/1‑143 (MQ=255)
                                                                                                                                gccgggccacGTTCACGACCGGTAATATTCGCCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTGGGTTTGGGTTTACCTACAAAAACCACAATCCCGGCATTTGc    <  1:288208/143‑1 (MQ=255)
                                                                                                                                  ccggccacgTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCa  <  1:251872/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
TGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCA  >  NC_000913/701929‑702201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: