Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,158,384 G→A W172* (TGG→TGA ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,158,3840GA90.5% 45.9 / ‑6.0 22W172* (TGG→TGAptsGfused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (8/11);  minor base C (0/2);  total (8/14)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

CGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGA  >  NC_000913/1158245‑1158526
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cGATCGCAGCGTACATGTTTGACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGaccg                                                                                                                                             <  1:220708/143‑1 (MQ=255)
 gATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGCCCTGGCTGCCATCTTTCCTGGCGTTGTGCTGTCCTTCCTTTGcccgc                                                                                                                                            >  1:122162/1‑143 (MQ=255)
  aTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTCAACGCTTTGTGCCGATCATGTCTGGCCTCGCTGCCATCTTTACTGGCGTTGTGATGTCCTTCATTTGaccccc                                                                                                                                           >  1:212340/1‑143 (MQ=255)
        gCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTgg                                                                                                                                     <  2:93904/143‑1 (MQ=255)
          gTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTGGtt                                                                                                                                   <  2:114965/143‑1 (MQ=255)
            ccgtGTTTTGCCGTTTCTACCGTGTTACGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGTTCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGCCCGCCGATTGGTTCt                                                                                                                                 <  1:364537/140‑1 (MQ=255)
             cgtGTTTAGCCGTTTCTACCGGATTAAGCTGCCTGAGTATCGTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTGGTTCTg                                                                                                                                <  1:133923/141‑1 (MQ=255)
                gTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTCCTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTGGTTCTGCaa                                                                                                                             <  2:426281/143‑1 (MQ=255)
                 tttAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCAGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTAATTTGACAGCCCATTGGTTCTGCaaa                                                                                                                            >  1:165748/1‑142 (MQ=255)
                  ttAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTGGTTCTGCAATc                                                                                                                           <  2:194135/143‑1 (MQ=255)
                             tACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTGGTTCTGCAATCCAGACCTtctc                                                                                                                >  2:171137/1‑143 (MQ=255)
                                      aaGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGgc                                                                                                       >  2:66846/1‑143 (MQ=255)
                                                 gTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTGGTTCTGCAATCGAGCCGGTCTCTCAGTGGGCTGCTTACCAGa                                                                                            >  2:17129/1‑143 (MQ=255)
                                                        ggCTGCTTTGCCGGTAAACGCGTTGGGCCGATCAGTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGt                                                                                     <  2:142445/143‑1 (MQ=255)
                                                          cttcttTGCCGGTAAACGCTTTGTGCCGTTCATTTCTGGCCTGGCTGCCATCATTACTGGCGTTGTGCTGTCCTACATTTGACCGCCGATAGGTCCTGCAATCCAGACCTTCTCGCAGTGGACTACTTACCAGAACCCgaaaa                                                                                   >  1:194426/1‑139 (MQ=255)
                                                            tcttTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTTGGCTGCTTACCAGAACCCGGTAGtt                                                                                 <  2:136588/143‑1 (MQ=255)
                                                               ttGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCCTCATTTGACCGCCCATTGGTTCTGCCATCCAGACCTTCCCCCAGTGGGCTCCTCACCCTAAccccccggctcgg                                                                              >  2:444979/1‑133 (MQ=255)
                                                                  ccGGTAAACGCTTTGTGCCGATCATGTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGTCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGttt                                                                           <  2:219712/143‑1 (MQ=255)
                                                                          cGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTa                                                                   >  2:98506/1‑143 (MQ=255)
                                                                                     gATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGACCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCg                                                        >  1:441452/1‑143 (MQ=255)
                                                                                       ttctttCTGTCTTGGCTGCCATCTTTTTTGGCGTTGTGCTGTCCTTCATTTGACCGCCGTTTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGTACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGaa                                                      <  1:411264/140‑1 (MQ=255)
                                                                                               ggcctggcGGGCCTCTTTAGCGCTGTGTTGCTGTCCCTCGGTTGACCGCCGATTGGTTCTGCGATCCATGCCTTGTCTCGGTGGGCTGCTTACCAGAAGCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCt                                              <  1:444979/143‑1 (MQ=255)
                                                                                                                     gCGTGGTGCTCTCGTTGCGTTGCCCGCCGATTGGTTCTTCCATCTAGTCCTTCTCTCAGTGTGCTGCTTACCAGACCCCGGCAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCGGGTACCGTTTGGTCTGcaccac                        <  1:396658/143‑1 (MQ=255)
                                                                                                                                  cTTCAGTTGACCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTAc           <  2:212340/143‑1 (MQ=255)
                                                                                                                                        ttGACCTCCGATTGGTTCTGCCATCCAGACCTTCTCTCAGTTGGCTTCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTcc     <  2:143562/143‑1 (MQ=255)
                                                                                                                                           gccgccGTTTGGTTCGGCATTCGAGACCTGCGCGCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGa  <  1:270526/143‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                              
CGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGA  >  NC_000913/1158245‑1158526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: