Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 701,378 +T coding (217/1221 nt) nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913701,3781.T90.0% 48.5 / 0.1 20coding (217/1221 nt)nagCN‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base T (8/10);  total (8/12)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTA  >  NC_000913/701249‑701507
                                                                                                                                    |                                                                                                                                 
ggTAATGTTCTTCAGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCTCCCGGTGGAGGc                                                                                                                       <  1:140944/143‑1 (MQ=255)
   aaTGTTCTTCTGCCAGCACTTTGCTGCTGATATCAAACAGAGTTATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCTCCCGGTGGAGGCctg                                                                                                                    <  1:230554/143‑1 (MQ=255)
      gTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATTGCGCGGCGGCCTCCCGGTGGAGGCctgctg                                                                                                                 >  1:307024/1‑143 (MQ=255)
             tctcagcagcaaagtgctgGGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCTCCCGGTGGAGTCCTGCTGATTAAct                                                                                                          >  1:305037/15‑143 (MQ=255)
                  tctcTTTGCTTGTGAGATGTATCAGAGTTTGTGTGGTTTCATTACGACCTAGTCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGCTAGCGCGGCGGCCTCCCGGTGGAGGCCTGCTGATCAACTTCttt                                                                                                     <  2:97050/140‑1 (MQ=255)
                   cACTTTGCTGCTGAGATCACACAGAGTGATGGTGGCGTCATGACGACCAAGCTGTACGCTGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCTCCCGGTGGAGGCCTGCTGATCAACTTCTTTg                                                                                                    <  1:19105/143‑1 (MQ=255)
                     cTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGAGAGCGCGGCGGCCTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGAt                                                                                                  <  1:139300/143‑1 (MQ=255)
                                 gaTCAAACTGAGTGATTGTGGCGTTATTTTGTTCCGCCCATACACCAAATGCGTGGAAATTGCGGGGTTCGGTGACGATGGAGATAGCGCGGCGGCCTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTc                                                                                      <  1:151541/143‑1 (MQ=255)
                                      aaaggagGGATGGGGGCGGCATGACGACAAAGCCGTACGCGGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGAGCCCTAGCGATCGAGGCATCCTTATCAACTTCTTTGATCAGCCCGCGTTCGATaa                                                                                 <  1:154374/139‑1 (MQ=255)
                                                      cGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAAtttt                                                                 <  1:319221/143‑1 (MQ=255)
                                                                         tatgctgATTGCGTTGATTTTTCTGTTTTTGGTGACTATGGAGATAGCGCGGCGGTCTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCa                                              <  1:149883/137‑1 (MQ=255)
                                                                           tgCCGAGTGCGTGTAAATTCCGGCGTTGGGTGACGATGTAGTTAGCGCGGCGGCTTCCTGGTGGAGGGCTGCTGATCAAATTCTTTGATCAGGCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAg                                            <  2:564/142‑1 (MQ=255)
                                                                                 ttGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCTCCCGTTGGAGGCCTGCTGATCAACTTCTGTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCt                                      <  1:107071/143‑1 (MQ=255)
                                                                                                   tttCGGTGACGATGGAGATAGCGCGGCGGCCTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCGCTCTGagt                    >  1:7024/1‑141 (MQ=255)
                                                                                                    ttCGGTGACGATGGAGATAGCGCGGCGGCCTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATc                   >  1:376268/1‑143 (MQ=255)
                                                                                                    ttCGGTGACGATGGAGATAGCGCGGCGGCCTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATc                   >  1:376622/1‑143 (MQ=255)
                                                                                                       ggTGACGATGGATTTTGCGCGGCGGCCTCCTGGTGGAGGTCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCcgc                <  1:264586/143‑1 (MQ=255)
                                                                                                         tGACGAGTGAGATAGCGCGCCGGCCTCCCGGTGGCAGCCTGCTGATCAACTTCCTTGATCCGCCCGCGCCCGACAAGCCGACGCCCAATTTTGCTTACGCTGGCGGGGGCAAGCTGCCCCTCCTCGCCAATCTGAAGCCGCga              >  2:167705/1‑143 (MQ=255)
                                                                                                              aTGGAGATAGCGCGGAGGCCTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGCTAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCCCGGACATCCGAATCCGAGCGAtcg         >  2:322984/1‑141 (MQ=255)
                                                                                                                     tAGCGCGGCGGCCTCCCGGCGCGGACCTGCTGATTAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCGCGTa  >  2:227774/1‑143 (MQ=255)
                                                                                                                                    |                                                                                                                                 
GGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTA  >  NC_000913/701249‑701507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: