| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 701,378 | +T | coding (217/1221 nt) | nagC ← | N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 701,378 | 1 | . | T | 90.0% | 48.5 / 0.1 | 20 | coding (217/1221 nt) | nagC | N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base T (8/10); total (8/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑C‑C‑CCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTA > NC_000913/701249‑701507 | ggTAATGTTCTTCAGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑C‑CTCCCGGTGGAGGc < 1:140944/143‑1 (MQ=255) aaTGTTCTTCTGCCAGCACTTTGCTGCTGATATCAAACAGAGTTATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑C‑CTCCCGGTGGAGGCctg < 1:230554/143‑1 (MQ=255) gTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATTGCGCGGCGG‑C‑CTCCCGGTGGAGGCctgctg > 1:307024/1‑143 (MQ=255) tctcagcagcaaagtgctgGGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑C‑CTCCCGGTGGAGTCCTGCTGATTAAct > 1:305037/15‑143 (MQ=255) tctcTTTGCTTGTGAGATGTATCAGAGTTTGTGTGGTTTCATTACGACCTAGTCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGCTAGCGCGGCGG‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCttt < 2:97050/140‑1 (MQ=255) cACTTTGCTGCTGAGATCACACAGAGTGATGGTGGCGTCATGACGACCAAGCTGTACGCTGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTg < 1:19105/143‑1 (MQ=255) cTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGAGAGCGCGGCGG‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGAt < 1:139300/143‑1 (MQ=255) gaTCAAACTGAGTGATTGTGGCGTTATTTTGTTCCGCCCATACACCAAATGCGTGGAAATTGCGGGGTTCGGTGACGATGGAGATAGCGCGGCGG‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTc < 1:151541/143‑1 (MQ=255) aaaggagGGATGGGGGCGGCATGACGACAAAGCCGTACGCGGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGAGC‑C‑CTAGCGATCGAGGCATCCTTATCAACTTCTTTGATCAGCCCGCGTTCGATaa < 1:154374/139‑1 (MQ=255) cGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAAtttt < 1:319221/143‑1 (MQ=255) tatgctgATTGCGTTGATTTTTCTGTTTTTGGTGACTATGGAGATAGCGCGGCGGTC‑T‑CCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCa < 1:149883/137‑1 (MQ=255) tgCCGAGTGCGTGTAAATTCCGGCGTTGGGTGACGATGTAGTTAGCGCGGCGG‑CTT‑CCTGGTGGAGGGCTGCTGATCAAATTCTTTGATCAGGCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAg < 2:564/142‑1 (MQ=255) ttGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑C‑CTCCCGTTGGAGGCCTGCTGATCAACTTCTGTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCt < 1:107071/143‑1 (MQ=255) tttCGGTGACGATGGAGATAGCGCGGCGG‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCGCTCTGagt > 1:7024/1‑141 (MQ=255) ttCGGTGACGATGGAGATAGCGCGGCGG‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATc > 1:376268/1‑143 (MQ=255) ttCGGTGACGATGGAGATAGCGCGGCGG‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATc > 1:376622/1‑143 (MQ=255) ggTGACGATGGATTTTGCGCGGCGG‑C‑CTCCTGGTGGAGGTCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCcgc < 1:264586/143‑1 (MQ=255) tGACGAGTGAGATAGCGCGCCGG‑C‑CTCCCGGTGGCAGCCTGCTGATCAACTTCCTTGATCCGCCCGCGCCCGACAAGCCGACGCCCAATTTTGCTTACGCTGGCGGGGGCAAGCTGCCCCTCCTCGCCAATCTGAAGCCGCga > 2:167705/1‑143 (MQ=255) aTGGAGATAGCGCGGAGG‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGCTAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCCCGGACATCCGAATCCGAGCGAtcg > 2:322984/1‑141 (MQ=255) tAGCGCGGCGG‑C‑CTCCCGGCGCGGACCTGCTGATTAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCGCGTa > 2:227774/1‑143 (MQ=255) | GGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑C‑C‑CCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTA > NC_000913/701249‑701507 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |