Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 704,008 C→A P22Q (CCG→CAG)  nagE → N‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913704,0080CA100.0% 38.0 / NA 17P22Q (CCG→CAG) nagEN‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (8/9);  total (8/9)

CGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAAACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAG  >  NC_000913/703870‑704142
                                                                                                                                          |                                                                                                                                      
ctgCAGTACAATTTGCAGCAAACTAAAAATACGTCTTGAAACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATGGCGGTGCTGCAGGTg                                                                                                                                    <  2:322229/141‑1 (MQ=255)
    aGTACAATTTGCAGCAAAATAAAAATACGGCTTGAAACGAGCCAACTAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTAGAGCTCCCTATTGTGGTTCTGCAGGTagcgg                                                                                                                                >  1:56840/1‑143 (MQ=255)
        cAATTTGCAGCAAAATAAAAATACGGCTTGAAACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTgggcgaccg                                                                                                                            >  2:297018/1‑136 (MQ=255)
            ttGCAGCAAAATAAAAATACGGCTTGAAACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTtg                                                                                                                        <  2:261785/143‑1 (MQ=255)
                               cGGTTTGAAACGAGCCAAATATGTTTTTTGTAGTGTGAATAAGATGAATATTTTAGGGTGTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAg                                                                                                     <  1:99923/143‑1 (MQ=255)
                                ttctccaaccacgCCGAATACTGTTATCGTAGGGGGAATAACATGAATATTTTAGGTTTGTTCCAGCGACTCGGTAGGGTGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCCGa                                                                                                    <  2:196470/131‑1 (MQ=255)
                                     gtaaCGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGTGCGTTATATTTTCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTAc                                                                                               <  1:16701/141‑1 (MQ=255)
                                          gAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTAGGTCAGCCAGATTTACTTAAc                                                                                          >  1:363366/1‑143 (MQ=255)
                                             ccAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTCGGGCGTTACAGCTCCCTATCGCGGTACTGCAGGTGGCGACACTGTTGATGCGATTCGGACAGCCAGAATTACTTAACGtt                                                                                       >  1:54102/1‑143 (MQ=255)
                                                     ggTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTGCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTAt                                                                               <  1:368319/143‑1 (MQ=255)
                                                        tctcGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGc                                                                            >  2:190352/1‑143 (MQ=255)
                                                                         gATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGAGTTACTTAACGTTGCGTTTATTGCCCACGCGGGCGGTGCGa                                                           >  2:505/1‑143 (MQ=255)
                                                                               tatTTTAGTTTTTTTCTAGCTTCTCTGTAGGGCGTTACAGCCCCCCATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTg                                                     <  1:54084/143‑1 (MQ=255)
                                                                                        ttttttCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCtag                                            >  2:275578/1‑141 (MQ=255)
                                                                                                   gtcTCGGTAGGGCGTTACAGTTCCCTATTGCGGTGCTGCAGGTGGCGGCATTGTTGTTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTc                                 <  1:266285/141‑1 (MQ=255)
                                                                                                               cGTTACAGCGCCCTAGCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTGGGCCAGATTTACTTGACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGtgtg                     <  2:81736/143‑1 (MQ=255)
                                                                                                                                  ggTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCCTCGCCATCGGTGTGGCATCCCGCTGGTCGAAAg  >  2:83588/1‑143 (MQ=255)
                                                                                                                                          |                                                                                                                                      
CGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAAACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAG  >  NC_000913/703870‑704142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: