Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,726,169 T→G F49L (TTT→TTG nemR → transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,726,1690TG100.0% 20.2 / NA 12F49L (TTT→TTGnemRtranscriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (5/7);  total (5/7)

ACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAA  >  NC_000913/1726035‑1726306
                                                                                                                                      |                                                                                                                                         
acCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTCCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTat                                                                                                                                   >  1:77185/1‑142 (MQ=255)
                             ggCGACGGGGGAGCAGCTGTGCCGGCAACGTGGAGTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCGGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGc                                                                                                      <  2:64883/143‑1 (MQ=255)
                                gACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAACCACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTATTTGCGCACTAAAAAAGAGTTTGGCGTTGCCATGCTTGAGCGTc                                                                                                   >  1:11895/1‑143 (MQ=255)
                                    ggggAGCAGCTTTGCTGGGACCGTCGGTTCACGCGCATGGGGCTAAGCGAGTGACTAAAAACCGCTGAACTGCCTAAAGGGTCCTTCTATCACTACTTGCGGTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTa                                                                                               <  2:383403/140‑1 (MQ=255)
                                        aGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACAACTTGCGCTATTATGTGGCGTTTAACAATACCATGCTTGAGCGTCATTAcgcc                                                                                           >  1:255191/1‑143 (MQ=255)
                                                                           gggCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAACGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAAta                                                        >  1:77304/1‑142 (MQ=255)
                                                                                  ggggaTTACTAAGAACCGCTGAAGTGCGGAGAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGaa                                                 <  2:63189/139‑1 (MQ=255)
                                                                                     aaTTACTAAAACCCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGt                                              <  2:197719/143‑1 (MQ=255)
                                                                                           tAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTAc                                        >  1:271103/1‑143 (MQ=255)
                                                                                                              gAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAATCGTTTGTTGTTGCTATGTTTTATTGTCATTATGTCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCtt                     <  1:354001/143‑1 (MQ=255)
                                                                                                                      ccTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCGATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAg             <  2:92024/143‑1 (MQ=255)
                                                                                                                                 tactTGCGCTCTAACGAAGCGTCTGGCGTTTCCATTCTTGAGCGTCATTTCGCTGTATATCACCAGCGACTCACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGaa  <  1:24826/143‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                         
ACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAA  >  NC_000913/1726035‑1726306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: