| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 701,378 | +T | coding (217/1221 nt) | nagC ← | N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 701,378 | 1 | . | T | 85.0% | 88.5 / 12.5 | 41 | coding (217/1221 nt) | nagC | N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base . (4/2); new base T (14/20); total (18/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑C‑CCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGC > NC_000913/701243‑701502 | gCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑GTG‑C‑C‑CCCCGt > 1:665661/1‑143 (MQ=255) tAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCct > 2:281746/1‑143 (MQ=255) ttgttctGCCACCCCTTTGCTGCGCTGATCACACAGCGTGATGGTGGCGTCATGACGCCCGCTCCGTACGCCGCTTGCGTCGTCA‑TTGCAGGTTTCGGTGACGATGGATATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGa < 1:714238/140‑1 (MQ=255) tctGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCaa < 2:372895/143‑1 (MQ=255) ccAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAActtc > 2:258898/1‑143 (MQ=255) ttttGCTGCTGAGATCAAACAGAGCGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGAt < 2:3929/142‑1 (MQ=255) cTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGAt < 1:304622/143‑1 (MQ=255) tttGCTGCTGAGATCAAACAGAGTGAAGCTAGCGTCATGTCGACCAAGCCGTACGCCGATTGCGAGGAAAGTTGC‑GGATCGGAGGACGAGGGAGATAGCGCGAC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGAGCAACGTCTGTGATc > 1:398454/1‑143 (MQ=255) tgctgAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGccc > 2:213733/1‑143 (MQ=255) gaTCAAACTGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTc < 2:708555/143‑1 (MQ=255) aTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGACA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTCCg > 2:322690/1‑143 (MQ=255) aTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCg > 2:107721/1‑143 (MQ=255) tCAAACAGAGTGATGGTGGCGTCATGACGACCAAGACGTAAGCCGATTGGGTGGAAA‑TTGCGGGTTTCGGTGAAGATGGAGATAGCGCGGC‑G‑G‑CAT‑CCCGGTGGAGGCCTGCTGATCAACTTCTTTGCTCAGCGCGCGCTCGa > 1:557497/1‑143 (MQ=255) aCAGAGGGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTGTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAg < 2:35673/143‑1 (MQ=255) gtggtggtgGCGTCGTGAGGGCCAAGCTGTGGGGCGATTGGGTGGAGA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGAc < 1:458038/143‑1 (MQ=255) tggtggtggGGTCAGGCCGGCGCAGGCGTACGCGGAGTGCGTGGAAA‑GTGCGGGTTTCGGTGACGAGGGAGATAGCGGGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGatg < 1:650250/140‑3 (MQ=255) tGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑GCC‑C‑CCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATACGCTGAcg > 2:583283/1‑143 (MQ=255) aTGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGGTCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGAcgcg > 1:407501/1‑143 (MQ=255) tCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTgg > 1:94261/1‑143 (MQ=255) cATGACGGCCAAGCTGTACGCCGATGGTGTTGAGA‑GTGCGGGTTGCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGCTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGt < 1:41970/143‑1 (MQ=255) aTGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGAC‑G‑G‑C‑CTCCCGGTGGAGGCCGGCTGATCAACTACTTTGATAAGCCCCGGTACGATAAGACTACGCGAAAATCTGGtg > 1:611474/1‑142 (MQ=255) tGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑G‑C‑T‑CCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTGCGGTAGGCTGACGCGTCATTTTGGTTa > 1:39472/1‑143 (MQ=255) tGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTa > 2:22494/1‑143 (MQ=255) gacgacCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTAc < 2:477632/143‑1 (MQ=255) cccaGCCGGCCGCCGACTGCGTGGCAA‑GTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTgg < 1:419853/140‑1 (MQ=255) aaGCCGTACGCCGATTGCGGGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑C‑CCCGGTGGAGGCCTGCTGATCAACTTCTTTGAGCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCgg < 2:214642/143‑1 (MQ=255) aGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCgg < 2:667587/143‑1 (MQ=255) aCGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCaa > 2:331442/1‑143 (MQ=255) cGCCGATGGCGTGGAAG‑TTGCGGGTTTCGGGGACGGTGGAGATAGCGCGGC‑G‑G‑CGT‑CCCGGTGGAGGCCCGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAg < 2:399891/143‑1 (MQ=255) gATTGCGTGGGAA‑TTGCGGGTTGCGGTGGCGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATGAGCGCGCGTTGGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTgg < 1:538698/143‑1 (MQ=255) ggggcgggacgtGCGGGTGTCGGTGACGATCGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACATCTTTGATCAGCCCGCGTGCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCtt < 1:386165/132‑1 (MQ=255) gTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTCCGCTGGCGGGGGCAAGATGGCTTTGc > 1:110514/1‑143 (MQ=255) cggtgcaacgccgcgTTTCGGCGACGATGGCGCTAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGACCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTATTCTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTg < 2:354702/129‑1 (MQ=255) gaca‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCg < 2:236452/140‑1 (MQ=255) acgtggCGGGTTCCGGTGACGTTGGAGATAGCGCGGC‑C‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCGTTGATCAGCCCGCGTTCGACAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCACCCTGGCTGGGCTCgg < 1:525112/138‑1 (MQ=255) gCGGGTTTCGGGGACGATGGAGATAGCGCGGC‑G‑G‑C‑CGCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATc < 1:8962/143‑1 (MQ=255) tGACGGTGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCGGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCga < 1:258898/143‑1 (MQ=255) gATGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTGCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTGCGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGGtt > 1:633926/1‑143 (MQ=255) aTGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTg < 1:107721/143‑1 (MQ=255) tGGAGATAGCGCGGC‑G‑G‑C‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATggg > 1:495192/1‑143 (MQ=255) gggtatggccgggc‑g‑g‑c‑CTCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTTGTTAAGCTGCGGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGc < 1:231985/132‑1 (MQ=255) | GCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAA‑TTGCGGGTTTCGGTGACGATGGAGATAGCGCGGC‑G‑G‑C‑C‑CCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGC > NC_000913/701243‑701502 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |