Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 704,008 C→A P22Q (CCG→CAG)  nagE → N‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913704,0080CA100.0% 100.5 / NA 36P22Q (CCG→CAG) nagEN‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/24);  total (12/24)

AGTACAATTTGCAGCAAAATAAAAATACGGCTTGAAACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGACAGC  >  NC_000913/703874‑704147
                                                                                                                                      |                                                                                                                                            
aGTACAATTTGCAGCAAAATAAAAATACGGCTTGAAACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTggcgg                                                                                                                                      >  2:102700/1‑143 (MQ=255)
 gTACAATTTGCAGCAAAATAAAAATACGGCTTGCAACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTggcggc                                                                                                                                     <  2:207271/143‑1 (MQ=255)
   aCAATTTGCAGCAAAATAAAAATACGGCTTGAAACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCAc                                                                                                                                   <  2:185195/143‑1 (MQ=255)
    cAATTTGCAGCAAAGTAGAAATACGGCTTGAAACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGATTATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACt                                                                                                                                  <  1:280062/143‑1 (MQ=255)
      aTTTGCAGCAAAATAAAAATACGGCTTGAAACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGt                                                                                                                                <  2:86882/143‑1 (MQ=255)
       tttGCAGCAAAATAAAAATACGGCTTGAAACGAGCCAAATAGGGTTCTCGAAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACGGtt                                                                                                                               >  1:368932/1‑143 (MQ=255)
               aaaataaaaataCGGCTTGAAACGAGCCAAATAGGGTTCTCGGAGGGGGAATAAGATGAATAATTTAGGATTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCCCGGTGCTGCAGGTGGCGGACCCGGTGCTGCGAt                                                                                                                       >  1:598397/1‑143 (MQ=255)
                          agggCCTGAAACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCa                                                                                                           <  1:38590/141‑1 (MQ=255)
                                 gAAACGAGCCAAATCGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTGCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTAc                                                                                                    <  1:45888/143‑1 (MQ=255)
                                 gAAACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTAc                                                                                                    <  2:606893/143‑1 (MQ=255)
                                             aTAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGt                                                                                        <  2:158451/143‑1 (MQ=255)
                                             aTAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTGCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGt                                                                                        <  1:641123/143‑1 (MQ=255)
                                             aTAGGGTGCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGt                                                                                        <  1:644111/143‑1 (MQ=255)
                                                   ttCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGCCGGGACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAAGGTTGCGTTTAtta                                                                                  >  1:437982/1‑142 (MQ=255)
                                                        gTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGTTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAg                                                                             >  2:619521/1‑143 (MQ=255)
                                                                            atTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGa                                                         <  2:356611/143‑1 (MQ=255)
                                                                              tttAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTACCGTTACGTTTATTGCCCAGGCGGGCGGTGCGTTTTTTGAtg                                                       >  1:248684/1‑142 (MQ=255)
                                                                              tttAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATa                                                       <  1:612119/143‑1 (MQ=255)
                                                                                 aGGTTCTGTCCAGCGCCGCTGTAGGGCGGTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAAcc                                                    <  1:595232/143‑1 (MQ=255)
                                                                                    ttttttCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCTGTGCTGCAGGTGGCGGCCCTGTTGTTCGATAGCACTCGGCTTGAGGGGCCCAGTGGTGCCTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCg                                                 <  2:170444/143‑1 (MQ=255)
                                                                                    ttttttCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGAGTCGGTCCGCCAGATTTACTTAACGTAGCGTTGCTTGCCCCGGCGGGCGGCGCGGTTCTGGATCGCCTCg                                                 >  1:719301/1‑143 (MQ=255)
                                                                                      ttttCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCa                                               <  1:585654/143‑1 (MQ=255)
                                                                                       tttCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCAt                                              <  2:502808/143‑1 (MQ=255)
                                                                                           cAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAAt                                          <  2:527754/143‑1 (MQ=255)
                                                                                           cAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTCCGTTTATTGCCACGGCCGGGGGTGCGATTGTTGATAACCTCGCATTaag                                          >  1:492695/1‑142 (MQ=255)
                                                                                             gCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCACGCGGCCGGTGCGATTTTTGATAACCTCGCATTAATCt                                        >  2:23417/1‑143 (MQ=255)
                                                                                              cggcTCGGCAGGGCGTTACGGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATcgt                                       <  1:298365/140‑3 (MQ=255)
                                                                                               gattgggTAGGGCGTTACAGCTCCCTATCGTGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTc                                      <  1:318312/138‑1 (MQ=255)
                                                                                               gagtCGGGAGGGCGTTACAGCTCCCTCTCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTc                                      <  1:471471/140‑1 (MQ=255)
                                                                                                 tggggTAGGGCGTTACCGCCCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGc                                    <  1:661196/140‑1 (MQ=255)
                                                                                                        gggCGTTACTGCTCCCTATCGCGGGGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGgt                             <  1:694461/143‑1 (MQ=255)
                                                                                                               aCAGCTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTGACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCAt                      >  1:173309/1‑143 (MQ=255)
                                                                                                                   cTCCCTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGAGTTACTTAACGTTGCGTTGATTGCCCAGGCGGGCGGTGCGGCATTTGATAACATCGCGCTACTCTGCGCCATCTGGGTGGCATcctg                  >  1:410997/1‑141 (MQ=255)
                                                                                                                    gcccTATCGCGGTGCTGCAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGc                 <  1:193472/142‑1 (MQ=255)
                                                                                                                            gcgGTGCTGCATGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGaa         <  1:77828/143‑1 (MQ=255)
                                                                                                                                   tgcAGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGACAgc  >  2:414152/1‑143 (MQ=255)
                                                                                                                                      |                                                                                                                                            
AGTACAATTTGCAGCAAAATAAAAATACGGCTTGAAACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGACAGC  >  NC_000913/703874‑704147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: