Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 705,596 C→T N551N (AAC→AAT)  nagE → N‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913705,5960CT98.2% 157.1 / NA 57N551N (AAC→AAT) nagEN‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (33/21);  minor base G (0/1);  total (33/24)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTAAACGTCTGGTGGAAGAGGGTGCGCAGGTAAGCGCAGGGCAAC  >  NC_000913/705457‑705732
                                                                                                                                           |                                                                                                                                        
ctACCGGTTATGTCGTGGCACTGCATCAGGTTCCTGCCGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGCGGCGGTGCACCCGACACATAAATTCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATacc                                                                                                                                       <  2:699202/142‑1 (MQ=255)
   ccGGTGATGTCGTGGCACTGGATGAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACCATCGTGGCAATCCTCAATaccaac                                                                                                                                    >  1:725214/1‑143 (MQ=255)
      gTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGCCACTCGTGCACATCTTCAATaccaacccc                                                                                                                                 >  2:109605/1‑141 (MQ=255)
       tGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTCCCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCAcg                                                                                                                                >  2:61925/1‑143 (MQ=255)
              gTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGCTCTg                                                                                                                         >  2:131045/1‑143 (MQ=255)
                       gATCGGGGTCCTGACGAAGCATTCGCCAGCAGAGTGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAAc                                                                                                                <  1:505152/143‑1 (MQ=255)
                        aTCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCCGCCGCAGGGACAATCGTGAAAATCTTCAATGCCAACCACGCGTTCTGCCTCGAAAcc                                                                                                               >  2:473936/1‑143 (MQ=255)
                         tCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCg                                                                                                              >  2:115556/1‑143 (MQ=255)
                           aGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGATGCGGTGGGCGGGGCGGTGAAACCGACAGATAAAATCGGCGTAGCACCAGCCGCAGGGCCAATCGTGAAAATCTTCAATACCAACCACGCGTGCTGCCGGGAAACCGaa                                                                                                            >  1:259977/1‑143 (MQ=255)
                            ggTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAAGCGAATGAGAAGATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGaaa                                                                                                           <  2:168114/143‑1 (MQ=255)
                             gTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTGGTATCACCAGCCGCAGGGACAATCGTGAAAACCTTCAATACCAACCACGCGTTCTGCATGGAAACCGaaaa                                                                                                          >  1:52840/1‑143 (MQ=255)
                             gTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCCACCACGCGTTCTGCCTGGCAACCGaaaa                                                                                                          >  2:303824/1‑143 (MQ=255)
                                  tGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGgcg                                                                                                     >  2:538082/1‑143 (MQ=255)
                                  tGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGgcg                                                                                                     >  2:586907/1‑143 (MQ=255)
                                     cgcaGCATTCGCCAGCAAAGCGGTGGGTGACTGTGTGGCGGTGAAACCGACAGATACAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCATCCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCgg                                                                                                  <  2:390767/140‑1 (MQ=255)
                                        aGCATTCTCCAGCAAAGCGCTGGGTGACGGTGGGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGgaga                                                                                               <  2:291615/143‑1 (MQ=255)
                                           aTTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAACAAGGCGCGGAGAtcg                                                                                            >  1:459607/1‑143 (MQ=255)
                                           aTTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGAtcg                                                                                            >  1:225710/1‑143 (MQ=255)
                                            ttCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGAtcgt                                                                                           >  1:287798/1‑143 (MQ=255)
                                                   gCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCat                                                                                    >  2:433669/1‑143 (MQ=255)
                                                       aGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGTAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATgg                                                                                <  2:474051/143‑1 (MQ=255)
                                                       aGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTAGCACCAGCCGCAGGGACAATCGTGAAAGTCTTCAATACCAACCTCGCGTTCTGCGTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATgg                                                                                <  2:327662/143‑1 (MQ=255)
                                                            tgggtgACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATc                                                                           >  2:553147/1‑143 (MQ=255)
                                                            tgggtgACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATc                                                                           <  2:176356/143‑1 (MQ=255)
                                                                  aCGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACAcc                                                                     >  1:605435/1‑143 (MQ=255)
                                                                         ggCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGCGACAGTTTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAgcgc                                                              >  2:572058/1‑143 (MQ=255)
                                                                          gCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGGTCGTCGTCCATATGGGTATCGACACCGTAGCGCt                                                             >  2:450329/1‑143 (MQ=255)
                                                                            ggTCAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTgg                                                           <  2:441992/143‑1 (MQ=255)
                                                                                    cGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTaaa                                                   >  2:591299/1‑143 (MQ=255)
                                                                                     gACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGACTGGAAACCGAAAAAGGCGCGGAGAACGTCGTCCATAAGGGTACCGACACCGTAGCGCTGGAAgggcaag                                                  >  1:11966/1‑138 (MQ=255)
                                                                                      aCAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATAGTCACCTTATCGCCGGAAGCTatacc                                                 >  2:529360/1‑139 (MQ=255)
                                                                                        aGATAACAGCGTCGTCTCGCCAGCCGCAGGGACAATCGTGAAAATCTTCACTACGAACCACGCGTTCTGCCTGGAAACCGAAAGAGGCGCGGAGATCGTCGTTCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCt                                               <  2:504055/143‑1 (MQ=255)
                                                                                           tAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGACGCCGAAAAAGGCGCGGAGCTCGTCGTCCATATGGGTATCTACACCGTAGCGCTGGAAGGTAAAGGCTTTa                                            >  2:514321/1‑143 (MQ=255)
                                                                                           tAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTa                                            <  2:195189/143‑1 (MQ=255)
                                                                                           tAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATACGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTa                                            >  2:24791/1‑143 (MQ=255)
                                                                                           tAAAATCGTCCCAGCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTa                                            <  2:674536/143‑1 (MQ=255)
                                                                                              aaTCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGCAGCGCTGGGAGGTAAAGGCTTTAAAc                                         >  1:476929/1‑143 (MQ=255)
                                                                                              aaTCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTGCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTAAAc                                         <  2:666357/143‑1 (MQ=255)
                                                                                                       aTCACCAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTAAACGTCtggtgg                                <  1:603745/143‑1 (MQ=255)
                                                                                                        gcACCAGCCGCCGTGACAATCGTAAAACTCTTCAACACCCACCCCTCCTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTAAACGTCTGGTGGa                               <  1:131045/142‑1 (MQ=255)
                                                                                                           ccAGCCGCAGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTAAACGTCTGGCGGAcga                            >  1:172728/1‑143 (MQ=255)
                                                                                                                 gCAGGGACAATCGGGAAAATCTTCAATACCAACCACGCGGTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTAAACGTCTGGTGGAAGAGGGTgc                      <  2:225658/143‑1 (MQ=255)
                                                                                                                   aGGGACAATCGTTAAAATCGTCAATACAGACTACGCGTTCTGCCGGAAAACCGAAAAAAGCGGGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGGAAAGGCTTTAAACGTCTGGTGTAAGAGGGTgcgc                    <  1:543221/143‑1 (MQ=255)
                                                                                                                   aGGGACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTAAACGTCTGGTGGAAGAGGGTgcgg                    >  2:588334/1‑142 (MQ=255)
                                                                                                                     ggACAATCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTAAACGTCTGGTGGAAGAGGGTGCGCAg                  <  2:715145/143‑1 (MQ=255)
                                                                                                                       aCAATCGTGAAAGGCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTAAACGTCTGGTGGAAGAGGGTGCGCAGGt                <  1:171198/143‑1 (MQ=255)
                                                                                                                         aaTCGTGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTACACGTCTGGTGGAAGAGGGGGCGGAGGTac              >  1:228200/1‑142 (MQ=255)
                                                                                                                            cgagAAAATCTACAATACCAACCACGAGTACAGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTAAACGTCTGGTGGAAGAGGGTGCGCAGGTAAgcg           <  1:71591/140‑1 (MQ=255)
                                                                                                                              tGAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTAAACGTCTGGTGGAAGAGGGTGCGCAGGTAAGCGCa         >  2:120893/1‑143 (MQ=255)
                                                                                                                               gAAAATCTTCCATACCAACCACGCGTTCGGCCTGGAGAGCGAAAAAGGCGCTGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTAAACGTCTGGTGGAAGAGGGTGCGCAGGTAAGCGCAg        <  1:61925/143‑1 (MQ=255)
                                                                                                                               gAAAATCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTAAACGTCTGGTGGAAGAGGGTGCGCAGGTAAGCGCAg        >  2:149635/1‑143 (MQ=255)
                                                                                                                                aaaaTCTTCAATACCAACCACGCGTTCTGCCTGGAAACTGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATGGACACCGTAGCGCTGGAAGGTAAAGGCTGTAAACGTCTGGTGGAAGAGGGTGCGCAGGTAAGCGCAgg       >  1:171885/1‑143 (MQ=255)
                                                                                                                                aaaaCCTTGAAAACCAACCCCGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTAAACGTCTGGTGGAAGAGGGTgcgcttacctgcgcacc       <  2:434488/143‑14 (MQ=255)
                                                                                                                                aaaaCCTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTAAACGTCTGGTGGAAGAGGGTGCGCAGGTAAGCGCAgg       <  2:225710/143‑1 (MQ=255)
                                                                                                                                   aTCTTCAAGACCCACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGGCGTCCATATGGGGAGCGACACCGTAGCGCTGGAAGGTAAAGGCTTTAAACGTCTGGTGGAAGAGGGTGCGCAGGTAAGCGCAGGGCa    <  1:681703/143‑1 (MQ=255)
                                                                                                                                     cTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTAAACGTCTGGTGGAAGAGGGTGCGCAGGTAAGCGCAGGGCAAc  >  2:438368/1‑143 (MQ=255)
                                                                                                                                     cTTCAATACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTAAACGTCTGGTGGAAGAGGGTGCGCAGGTAAGCGCAGGGCAAc  >  2:43245/1‑143 (MQ=255)
                                                                                                                                           |                                                                                                                                        
TTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTAAACGTCTGGTGGAAGAGGGTGCGCAGGTAAGCGCAGGGCAAC  >  NC_000913/705457‑705732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑