Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,976,907 A→C E104A (GAG→GCG)  galR → galactose‑inducible d‑galactose regulon transcriptional repressor, autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,976,9070AC97.7% 127.0 / NA 44E104A (GAG→GCG) galRgalactose‑inducible d‑galactose regulon transcriptional repressor, autorepressor
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (27/16);  total (28/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGC  >  NC_000913/2976781‑2977046
                                                                                                                              |                                                                                                                                            
tcggtcTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAg                                                                                                                              <  1:119723/143‑1 (MQ=255)
tcggtcTGGTCGTCGGTGCCGGTTCCGCTCCGCTTTTCGGTGCAATGGTGAATGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGGTTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAg                                                                                                                              <  1:488154/143‑1 (MQ=255)
    tcTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAAc                                                                                                                          >  1:409186/1‑143 (MQ=255)
        gTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCACTGAGCAACTGAt                                                                                                                      >  1:198402/1‑143 (MQ=255)
           gTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGCTGGCTTATCACACCGGTAATTTTTTATTGATTGGCACCGGTTACCACCACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTgcaccc                                                                                                                   >  1:311719/1‑138 (MQ=255)
               gTGATGGTTCCTATCCTTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCAGTAATTTTTTATTGATAGGCAAGGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCAt                                                                                                               <  1:330050/143‑1 (MQ=255)
                tGATGTTTCGGATCCGTTTTTGGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTGATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATc                                                                                                              <  1:535895/143‑1 (MQ=255)
                   tGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAGCGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCt                                                                                                           >  1:425549/1‑143 (MQ=255)
                         cGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGCTCCGCCATCGCTGtgctg                                                                                                     >  2:130379/1‑143 (MQ=255)
                         cGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTCAATTCATTTGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAAGTGATCCGCCATCGCTGtgctg                                                                                                     >  2:123000/1‑143 (MQ=255)
                          gATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGCGTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGtgctgc                                                                                                    <  2:323541/143‑1 (MQ=255)
                            tCCGTTTTTCGGCGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGt                                                                                                  <  2:585332/143‑1 (MQ=255)
                                tttttCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGgt                                                                                              >  2:269644/1‑143 (MQ=255)
                                tttttCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCGGCGTTGgt                                                                                              >  2:283428/1‑143 (MQ=255)
                                 tgttCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTGATCACACCGGTAATTGTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGgtc                                                                                             <  1:796481/141‑1 (MQ=255)
                                       gTGCAATGGTGAAAGCGGGCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTAACACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCAGCGCTGTGCTGCGTTGGTCGTCCAt                                                                                       >  1:31791/1‑143 (MQ=255)
                                          cAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGcc                                                                                    >  2:435709/1‑143 (MQ=255)
                                                  aaaGCGGTCGAACAGGTGGCTTATCAAACCGGTAATTTGTTATGGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCACAATGAt                                                                            >  2:820817/1‑143 (MQ=255)
                                                      cGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCAGGCCAAACTGATcccc                                                                        >  1:558292/1‑142 (MQ=255)
                                                        gTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGa                                                                      <  2:425549/143‑1 (MQ=255)
                                                            aaCAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGcc                                                                  >  2:807100/1‑142 (MQ=255)
                                                              cAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGTTTACCACAACGAACAAGAAGCGCGTCAGGACATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGa                                                                <  2:345918/143‑1 (MQ=255)
                                                                 gTGGCTTATCACACCGGTAATTTTTTATTGATTGTCAACGGTTACCACAACGAACAAAAAGCGCGTCAGTCCATTGAGCAACTGATCCGCCATCGCTGTGCAGCGTTGATCGACCATGCCAAAATGAACCCGGacaccgatcc                                                             >  1:282545/1‑134 (MQ=255)
                                                                      ttATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGCTGCTGATTTA‑GCCt                                                       >  2:67837/1‑143 (MQ=255)
                                                                          cacaCCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGTCAGTGTACGTTTTCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCAtt                                                   >  2:397691/1‑143 (MQ=255)
                                                                          cacaCCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCAtt                                                   <  2:409186/143‑1 (MQ=255)
                                                                           gcacCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGGACAAAAAGCGCGTCAGGCCATTGAGCAACGGATCCACCATGGCTGGGCTGCGTGGGTCGTCCATGCCGGAGGGATCACGAGTGCTGGTTTA‑GGGTCATTa                                                  >  1:697621/2‑143 (MQ=255)
                                                                           acacCGGTAATTTTTTATTGATTGGCAACGGTGACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTa                                                  <  1:546329/143‑1 (MQ=255)
                                                                            cacCGGTAATTTTTTAGTGATTGGCAACGGTTACCACAACGAACAAGAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTaa                                                 <  1:277340/143‑1 (MQ=255)
                                                                                 gTAATTTTATCTTGTGTGGCCCCGGTTACCACAACGCACAATCTGCGCGTCAGGCCATTCAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGaaa                                            <  2:30567/143‑1 (MQ=255)
                                                                                          tATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCCAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGccc                                   >  2:551008/1‑143 (MQ=255)
                                                                                          tATTGATTGGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑CCCTCATCAATGAAACAAATGcgc                                   >  1:314241/1‑141 (MQ=255)
                                                                                             tgattgGCAACGGTTACCACAACGAATAAAAAGCGCGTCAGGCCAGTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGCCCGGt                                <  2:296186/143‑1 (MQ=255)
                                                                                               attgGCAACGGTTACCACAACGAGGAATAAGAGAAACAAGCCAATGAGCAACTGAGCCGACATCGCTCTGCAGCGTTGGTCGGCAACGCCAAAAAGATCGCGGATGC‑GAGTTACGCCACAGTAATAAAAGAAAAGCAAGGTAt                              >  1:707855/1‑143 (MQ=255)
                                                                                               attgGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGCCCGGAAt                              >  1:453618/1‑143 (MQ=255)
                                                                                               attgGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAAGGAAACAAATGCCCGGtgt                              >  1:853344/1‑141 (MQ=255)
                                                                                                ttgGCAACGGTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCGGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGCCCGGGATg                             >  1:721678/1‑143 (MQ=255)
                                                                                                 tgacAACGGTTACCACAACGAATAAAAAGCGCGTCAGGCCATTGAGCAACGGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGCCCGGTATgg                            <  2:733853/140‑1 (MQ=255)
                                                                                                         gTTACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGCCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCACATTAATCAAACAAATGACCgggctgggacgcgcc                    >  1:308671/1‑130 (MQ=255)
                                                                                                          ttACCACAACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTTGTCGTCCATGCCACACTGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCa                   >  1:651740/1‑143 (MQ=255)
                                                                                                             ccACAACGGCCAAAAAGCGCGTCCGGCCAGTGGGCAACGGCGCCGCCATCGCTGTGCTGTGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAAcc                <  1:425462/143‑1 (MQ=255)
                                                                                                               acaACGAACAAAAAGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGt              >  2:328585/1‑143 (MQ=255)
                                                                                                                         aaaaGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATAAACCGTAGcctggctg    >  1:54640/1‑143 (MQ=255)
                                                                                                                           aaGCGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGc  >  2:492434/1‑143 (MQ=255)
                                                                                                                              |                                                                                                                                            
TCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTA‑GCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGC  >  NC_000913/2976781‑2977046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑