Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,726,004	G→A	intergenic (‑84/‑19)	ydhL ← / → nemR	DUF1289 family protein/transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,726,004	0	G	A	100.0%	110.0 / NA	39	intergenic (‑84/‑19)	ydhL/nemR	DUF1289 family protein/transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/15);  total (24/15)

AAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGT  >  NC_000913/1725869‑1726141                                                                                                                                        |                                                                                                                                          aaaTTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATctacta                                                                                                                                    <  2:206789/143‑1 (MQ=255) aaaTTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATctacta                                                                                                                                    >  2:304741/1‑143 (MQ=255)  aaTTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCtactac                                                                                                                                   >  2:552404/1‑143 (MQ=255)           gCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACg                                                                                                                          >  2:861870/1‑143 (MQ=255)              cGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCAt                                                                                                                       >  2:519486/1‑143 (MQ=255)                       gACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATctact                                                                                                                                     <  2:661503/120‑1 (MQ=255)                       gACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATctact                                                                                                                                     >  1:661503/1‑120 (MQ=255)                         cGGGCAAGCACTCTCATTGCTCCGCCACTTCGCCCTCCTCTGCTACGATTATTACCATTCTTGATGCTGTTCATGTCCATAGTAGCCACTTTGCTTGCACTAGACCGACTGATCTTCTACTCTCCTACGCATGAACAAactca                                                                                                            <  1:235288/143‑1 (MQ=255)                          gggAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAacacac                                                                                                           >  1:67535/1‑143 (MQ=255)                            gAAAGAACTCTAATTGCTCCGCCACTTCGCCGTCCTCAGATAAGATGATTACCATTATTGAAGCTGTGAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGACCAAACCCACCg                                                                                                         >  2:602812/1‑143 (MQ=255)                               aGAACTCTAATTGCTCCGCCACTTCCCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAACGTAGCAACTTTGCTTGCACTAGCCCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGaaa                                                                                                      >  1:847268/1‑142 (MQ=255)                                   ctctAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGa                                                                                                  <  1:278164/143‑1 (MQ=255)                                   ctctAATTGCTCCGCCACTTCGCCCTCCCCAGTTCAACACACGTCACGGATTGAAGCTGTTAAAGACCAAAGTAGCAACTTCGCTAACAACACACAGACTGATCTACTACACCACAACGCATGAACAAACACACCgcgcacca                                                                                                  >  1:842451/1‑136 (MQ=255)                                    tctAATTGCTCCTCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGAt                                                                                                 <  1:848883/143‑1 (MQ=255)                                        aTTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTc                                                                                             <  2:289288/143‑1 (MQ=255)                                          tGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTcgc                                                                                           <  2:261118/143‑1 (MQ=255)                                               cGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACa                                                                                      >  2:186476/1‑143 (MQ=255)                                                 ccACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACCGGATAGTCGCGAACAtc                                                                                    >  2:690307/1‑143 (MQ=255)                                                 ccACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGAACTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCACACACAtc                                                                                    >  1:129594/1‑143 (MQ=255)                                                    cTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGGTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTcc                                                                                 >  2:592926/1‑143 (MQ=255)                                                       cGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTgg                                                                              <  1:111220/143‑1 (MQ=255)                                                        gCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGc                                                                             <  1:552404/143‑1 (MQ=255)                                                               tgtgagatggttattaCCATTATTGAAGTGGGTAAAGTCCAAAGTAGCAACTTTGCTGGCACTAGACCGACTGATCTACTACACGCCAACGCATGAACGAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGc                                                                      <  1:298659/133‑1 (MQ=255)                                                                    tAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATGTACGACACTCCAACGCATGAACACACAAATCGAACATGATACTCTCGAACATCTCCTGACGACCGGCGAGCa                                                                 >  1:297946/1‑143 (MQ=255)                                                                        attattACCATTATTGAAGCTGTCAATGTCCAAAGGAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCCACGCATGTACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACtt                                                             <  1:444776/143‑1 (MQ=255)                                                                           attaCCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGc                                                          <  1:861870/143‑1 (MQ=255)                                                                             tggctgttgttaaccTGCTAATGTCCAAGGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACCCTCCAACGCATGAACAAACACCCCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCt                                                        <  1:334245/129‑1 (MQ=255)                                                                                cATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACACGACACTCGCGAACCTCTCCTGGCGACGGGCGAGCAACTTCGCCGGCa                                                     >  1:96945/1‑143 (MQ=255)                                                                                 attattGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACGTTGCCTGCag                                                    >  2:468057/1‑142 (MQ=255)                                                                                                     ccAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTCGCCTGCAACGTGGATTCACCGGGATggg                                >  1:776170/1‑143 (MQ=255)                                                                                                      cAAAGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCCCCGGGATgggg                               >  2:802353/1‑143 (MQ=255)                                                                                                       aaaGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGc                              >  1:759760/1‑143 (MQ=255)                                                                                                       aaaGTAGCAACTTTGCTTGCACTAGACCGACTGATCTACTACACGCCAACGCATGAACAAACACACCGAACATGATACTAGCGAACATCTCCTGGCGCCGAGCGAGCAACTCTGCCTGCAACATGGAGTCAGCGGGATgggga                              >  1:249323/1‑142 (MQ=255)                                                                                                                  tttGCTTGCACTAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTa                   >  2:61697/1‑143 (MQ=255)                                                                                                                  tttGCTTGCACTAGACCGACTGATCCACTACACTCCAACGCATGAACAAGCACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTa                   <  2:347712/143‑1 (MQ=255)                                                                                                                             tAGACCGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAACAACCGc        >  2:39342/1‑143 (MQ=255)                                                                                                                                 ccGAATGATCTACTACACTGCAACGCATGAACGAACACACCGAACATGATACGCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGaa    <  2:67535/143‑1 (MQ=255)                                                                                                                                  cGACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAg   >  2:488394/1‑143 (MQ=255)                                                                                                                                   gACTGATCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGCTGGGGCTCAGCGCCCTACTCAACACCGCTGAAGt  >  2:104585/1‑143 (MQ=255)                                                                                                                                        |                                                                                                                                          AAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGT  >  NC_000913/1725869‑1726141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑