| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,726,876 | C→T | P73L (CCT→CTT) | nemA → | chromate reductase, quinone reductase, FMN‑linked, N‑Ethylmaleimide reductase, old yellow enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,726,876 | 0 | C | T | 97.6% | 120.9 / NA | 41 | P73L (CCT→CTT) | nemA | chromate reductase, quinone reductase, FMN‑linked, N‑Ethylmaleimide reductase, old yellow enzyme |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (20/20); minor base A (1/0); total (21/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCTGCGCAGTATTGAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAG > NC_000913/1726741‑1727012 | ggcagcgcAGGAATGAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATc < 2:25755/139‑1 (MQ=255) gtctggtgctgtgTGGCATTGCCACCCCGCGGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCg < 2:393256/131‑1 (MQ=255) gTATTGAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCg < 2:652188/143‑1 (MQ=255) aTTGAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGAGTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGa > 1:270292/1‑143 (MQ=255) ttGAACCGGGTGACACTCCAACAAAGGAGGTGGCGGAAGACTATCGCCAACGTGCCAGTGCCGGTTTGCTTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCACGAGGATGTGCAGGTGCGCTTGTCATCCATAGCCCGGAg > 1:107631/1‑143 (MQ=255) tGAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGCTTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCATGGCCTccctggccaggagg > 1:598364/1‑131 (MQ=255) tGAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGc > 2:629810/1‑143 (MQ=255) ccGGGTGACATTCCTGCCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAAt < 2:381327/143‑1 (MQ=255) cATTCCTACCCCGTTGATTGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCAt < 1:2685/143‑1 (MQ=255) ccTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGaa > 1:377532/1‑143 (MQ=255) gcccGGTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGaaaaaa < 2:565739/142‑1 (MQ=255) gggCAGAATAATATCGACAACGGGCCAGTGCCGGTTTGATTACTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCt < 2:26435/142‑1 (MQ=255) gCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTgg > 1:269182/1‑143 (MQ=255) gAATACTATCGCCAACGTGCCAGTGCCGGTTTGCTTATTAGTGACGCCACGCAAATTTATGCCCAGGCAAAAGGATATGCAGGTGCGATTGGCATCCATAGTCCGGAGCAAATTCCCGCATGGAAAAAAATCACGGCTGGCgc > 1:115310/1‑142 (MQ=255) tatatccAACGTTCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAAGTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGc < 1:353840/138‑1 (MQ=255) tATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGc > 2:124205/1‑143 (MQ=255) tATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGc > 1:610278/1‑143 (MQ=255) cGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTgc > 1:798873/1‑142 (MQ=255) ccAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAACAAAATCACCGGTGGCGTTCCTGCTGgaa > 1:564872/1‑143 (MQ=255) aCGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATg > 2:103228/1‑143 (MQ=255) tGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTc < 1:196375/143‑1 (MQ=255) aGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCatat < 2:270292/143‑1 (MQ=255) tGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAACGGTCATATgg > 2:176716/1‑143 (MQ=255) tttGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGc > 1:684406/1‑143 (MQ=255) aGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGca < 2:823916/143‑1 (MQ=255) aGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGca > 2:159068/1‑143 (MQ=255) cACGCAGATGTCGGCCTGGGGAAAGGGCTATGCAGGGGCGGTTGGCATCCATAGTCGGGAGCAAATGGCCGTATGGAAAAAAATCACCGCGGGCGTGCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGAc < 1:370432/143‑1 (MQ=255) aCGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACg > 2:278210/1‑143 (MQ=255) aTTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCTCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTtc < 2:772620/143‑1 (MQ=255) tttCTGCCCAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCAGCATGGAAATAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTtct > 2:772838/1‑143 (MQ=255) gcggacgacgCAAAAGGATATGCATGTGCGCTTGACATCCATAGCCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCa < 1:407888/134‑1 (MQ=255) naGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGCGCAAATTGCCACATCGAAAAAAATCACCGCTGGCGTTCATGCTGAAGCTGGTCATATGGCCGTGCAGCCGTCGCCCCCCGGCCGCCTTTCTCAcccgcg > 2:164250/2‑138 (MQ=255) cAGGCAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAg > 1:503084/1‑143 (MQ=255) ccattgggATATGCAGGTGCGCTTGGCAGCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCt < 2:190102/137‑1 (MQ=255) cAAAAGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCACTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTg < 2:377532/143‑1 (MQ=255) aaaaGGATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCCTTTCTCACGCCAGCCTGc > 2:826663/1‑143 (MQ=255) aaGCATATTCCGGGGCGCTTGGCATCCATAGTCCGGATGAAATTGCCGAATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCaa < 1:357918/143‑1 (MQ=255) gATATGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCt > 2:419594/1‑143 (MQ=255) tGCAGGTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCg < 1:815471/143‑1 (MQ=255) ggTGCGCTTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAAGCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACAGGCCGAATTTATCACGCCAGCCTGCAACCTGGCGGTCa < 1:525178/143‑1 (MQ=255) gTGCGCTTGGCATCCATAGTCCGGAGCAAACTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAg < 2:296517/143‑1 (MQ=255) | GTCTGCGCAGTATTGAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTTTGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAG > NC_000913/1726741‑1727012 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |