| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 97.6% | 124.9 / NA | 43 | I204S (ATC→AGC) | wcaA | putative glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (23/18); minor base T (0/1); total (24/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCT > NC_000913/2132648‑2132922 | gATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGACTTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGctct > 2:212460/1‑143 (MQ=255)gATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGctct < 1:180759/143‑1 (MQ=255) tAGAGGGTAAACAGCTGTTATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCTGCAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc < 1:97128/143‑1 (MQ=255) gggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCctct > 1:550277/1‑143 (MQ=255) tgTACACAGCTGATATTTTTTGCTGGCGCGGTTGAATTGGTCTTTGTGCTTGCGGTAAGAGTGGAAGTACCCGGAGAATTTTTT‑CGCTTACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt < 1:421223/142‑1 (MQ=255) ggTCAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt < 1:168967/143‑1 (MQ=255) ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAAGTGATCTGCATTTCACCGTGACTGATATGCAGGCACTGCGTCGCcactc > 1:41611/1‑142 (MQ=255) aaaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa < 1:335987/143‑1 (MQ=255) ggcaagatgcgtcgCTGGCGCGGTCGAATCTGGCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTc < 2:212735/130‑1 (MQ=255) cTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTcc > 1:228215/1‑143 (MQ=255) tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa > 2:540717/1‑143 (MQ=255) atatTTTTTGCTGGCGCGGTCGAATTTGTCTTGGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTGG‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACg < 2:494806/143‑1 (MQ=255) atTTTTTGCTGGCGCGGTCGACTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGCTGGAGATGCAGGCGCTGCGTCGCCTCTTCTACTTTCCACGGt > 2:566077/1‑143 (MQ=255) ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt > 2:84346/1‑143 (MQ=255) tttgGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCg < 1:195142/143‑1 (MQ=255) tttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCCGGGTCTGCGTCGCCTCTTCTCCTTTCGACGGttag > 1:325357/1‑141 (MQ=255) ttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACAGTCATTGATATGCAGGCCCACCGTACGCTCTTTTACTTTCCACGGTACGcc > 2:319927/1‑143 (MQ=255) cggccggggTCGACTTGGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGTGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATTTGCGTGTTCTTGCTCCCCACCACTACTTTCCACGGTTCGCCGTa < 1:707864/136‑1 (MQ=255) gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:390809/1‑143 (MQ=255) tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa > 2:466911/1‑143 (MQ=255) ttGCGGTAAAAGTGAAAGTACCCTGAGAATCTTTT‑CGGTGACGAGGCGATCTGCATTTCACCGTGATTGATATGCAGGCTATGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc < 2:843882/143‑1 (MQ=255) ggTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACAAACATCCTCAAGAAGATGTCGTaa > 2:555770/1‑143 (MQ=255) tAAACGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATc < 2:294689/143‑1 (MQ=255) tAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAGGCTGTCGTAATc > 2:247472/1‑143 (MQ=255) tAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGACGATGTCGTaacc > 1:274865/1‑141 (MQ=255) aaGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtg > 1:392501/1‑143 (MQ=255) ggAAGCACCCGGAGAATTTTGT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGcc < 2:133814/143‑1 (MQ=255) ccGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCCGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCGTCCGCCGGAGGATGTCGTAATCCTGTGCCGCTTTccg > 2:34890/1‑141 (MQ=255) cGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGt < 2:228215/143‑1 (MQ=255) ggAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGACGATGTCTTCGTCCTTTGCCTCTTTCAttt > 2:817385/1‑143 (MQ=255) gagaATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCCCCGTACTCCCCCCCCCTCCGCAGGCAGATGTCGTAAGCCTGTCCCGCCTTCCGtcc > 1:580835/1‑141 (MQ=255) tgtttttt‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGt < 1:80023/141‑1 (MQ=255) tttttt‑CGTTTATGAGGTGATCTGCATTTCATCGTTATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCTCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAt < 1:69111/143‑1 (MQ=255) tt‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa < 2:550277/143‑1 (MQ=255) gTGACGAGGTGATCTGCATTTCGCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGTCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa < 1:795844/143‑1 (MQ=255) gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa < 1:84346/143‑1 (MQ=255) gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACGTTCCACGGTTCGCCGTACTCGACCACCATCCGCGGGAAGATGTCGTAATCCTGTGCCGCTTTCCGCTCGGTCTAGagcaaa > 1:146525/1‑143 (MQ=255) aCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTCCGCCGTACTCCACCACCATCCGCCGGCCGATGTCCGAGCCCGGTCCCGCGTTCGGTGCGGCCTCGAACCAacac > 2:93001/1‑143 (MQ=255) aGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGCTGTCCTAATCCTGTGCCGCTTTCCGCTCCGTATCGCACACACActct > 1:681678/1‑143 (MQ=255) ggTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTCCAGATCGCTATCGAACAAACACTCtt > 1:31167/1‑143 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCCGACAGCGCGGCATCGAACAAACACTCTATGac > 1:486502/1‑142 (MQ=255) tgattgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTGTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGt > 2:188266/1‑143 (MQ=255) ttgatAGGCAGGCGCTGCGTCGCCTCTGCTACTGGCCACGGTGCGCCGTACTCCACCAGCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGGAGCGAACAAACACTCGTGGAAACGCCATGCCCAGGTaaa < 1:166512/143‑1 (MQ=255) atGCAGGCTCTGCGTCTCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCt < 1:367511/143‑1 (MQ=255) atGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTACACCACCATCCGCAGGACGATGTCGTAAGCCTGTGCAGCTTTCAGTTCGGTATCGAACAAAAACTCTTTGAACCGACATCCCCAggcacagccat > 1:318320/1‑134 (MQ=255) | GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTT‑CGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCT > NC_000913/2132648‑2132922 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |