Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,189,484:2 | +C | 13.9% | coding (4136/4224 nt) | rpoC → | RNA polymerase, beta prime subunit |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,189,482 | 2 | . | C | 13.9% | 384.0 / 72.9 | 115 | coding (4134/4224 nt) | rpoC | RNA polymerase, beta prime subunit |
Reads supporting (aligned to +/- strand): ref base . (38/61); new base C (5/11); total (43/72) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.82e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 |
Only 100 of 124 total aligned reads displayed.
TCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTCCTGTGTAA > NC_000913/4189195‑4189766 | tctgGCGTCAATATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTc < 2:626042‑M1/289‑2 (MQ=255) ctcGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTCTGCGTCGCCGGGCTGCGGGTGAAGCCcc > 2:370773‑M1/1‑287 (MQ=255) ctcGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTcc > 1:220510‑M1/1‑287 (MQ=255) gcgTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTccggc < 1:107043‑M1/289‑6 (MQ=255) cgTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGGTAAGCTccggcg > 2:885427‑M1/1‑283 (MQ=255) cgTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATAGTATGGGTCGCACGGCTGCGGGGGAAGCTccgtgt > 2:839437‑M1/1‑283 (MQ=255) ggcaaGATCGCAAACCGCGAACTGGAAGCTAACGGCAAAGTGGGTGCAGCTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTccggccc < 2:199010‑M1/287‑8 (MQ=255) aGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCAcc > 1:242262/1‑289 (MQ=255) aGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCC‑GGAGACCAGTAGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGAGGCCCGAAAGAGAACGTTACCGTTGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACAACCAGGATCGTATGCGTCGCCGACGTGCGGGTGGAGCT‑‑CCGGCCGCAcc > 1:390048/1‑288 (MQ=255) tCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCg < 2:220510/287‑1 (MQ=255) tCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCa < 1:370773/289‑1 (MQ=255) aaCCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑Ga < 2:145379/289‑1 (MQ=255) aaCCGCGAAAGGGAAGGGAACGGCAAAGGGGGGTCAACTTACTCCCGCTATCTGCTGGTTATAACCAAAGCGTCTCG‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑Ga < 2:453586/289‑1 (MQ=255) gTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGG‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAg > 1:407932/1‑289 (MQ=255) gCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGaa < 1:29230/288‑1 (MQ=255) cAACTTCATCCCCGGATCTGCTGGGTATCACCAAAGCGTCCCT‑GGCAACCGGGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGaa < 2:242262/288‑1 (MQ=255) aCTTGCTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAActg < 1:642063/289‑1 (MQ=255) aCTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGa > 2:789437/1‑289 (MQ=255) tCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACg < 2:585910/289‑1 (MQ=255) ccccCGATCTGCTGGGAATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCAGCATCTGTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTCCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACATTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCAGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACg < 1:769992/289‑1 (MQ=255) cccGCGATCTGCTGGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACg < 1:804705/289‑1 (MQ=255) cccGCGATCTGCTGGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACg < 1:563890/289‑1 (MQ=255) ccccgggaggttgggaagcgccAAAGCGTCTCG‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTCCCAGGAGACCACTCGCGTGCTGACCGAGGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCCCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACg < 2:159033/269‑1 (MQ=255) cgATCTGCTGGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGt < 2:711599/240‑1 (MQ=255) tgctgGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑ccg > 1:214136/1‑248 (MQ=255) tgctgGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑ccg < 2:214136/250‑3 (MQ=255) tgGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCCCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTggg < 1:909005/288‑1 (MQ=255) gTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTccggcccggctgcaccgcaggtgactgcagaagacgcatctgccagcctggcagaactgctgaacgcaggtctggg < 1:330617‑M1/288‑77 (MQ=255) tATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGtt < 2:265830/288‑1 (MQ=255) tccACCAGAGCGT‑TCTCGGCAACCGGGTACTTCATATCCGCGGCATCGTTCCAGGAGACCACTCGCGGGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCCCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGtt < 2:406804/286‑1 (MQ=255) cACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGTGACTGCAGAAGACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTgg > 1:313006/1‑288 (MQ=255) cACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGa > 1:233809/1‑289 (MQ=255) cACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGAt > 1:604505/1‑289 (MQ=255) aaGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACg > 2:649882/1‑289 (MQ=255) tctcT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCAAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCGGATAACGAGTaa > 1:638246/1‑289 (MQ=255) gCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGtt < 1:69932/288‑1 (MQ=255) ccGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCCCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGC‑CGCCTCTGCCAGCCTGGCAGAACTCCTGAACGCCGGTCTGGGCGGTTCTGACAACGAGTAATCGCTatcccttatacacctctg > 2:24761/1‑271 (MQ=255) gaCCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGGGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACCGCTGAAAGCCGGTCTGGGGGGTTCTGATAACGGGTAATCGTTAATCCGCAAATAACGTAAAAACCCCCCTCCGCGGGtttt > 2:20521/1‑289 (MQ=255) cTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTggg < 1:405937/221‑1 (MQ=255) cTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTggg > 2:405937/1‑221 (MQ=255) cTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTAt > 1:118798/1‑289 (MQ=255) gTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATgggg < 1:263977/288‑1 (MQ=255) gTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATgggg < 1:789437/288‑1 (MQ=255) gTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATgggg < 2:595032/288‑1 (MQ=255) ggCTGACCGAAGCGGCCGGTGCGGGCAAAAGCAGCGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCCCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATgggg < 2:317718/287‑1 (MQ=255) aCCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGttt > 1:107246/1‑289 (MQ=255) cGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAg > 1:110067/1‑289 (MQ=255) aaGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATgggg > 1:535579/1‑278 (MQ=255) agcaccCGTTGCGGGACAACGCCACGAACTGCGCGGCCTGAAAGAGAACGTTATCGGTGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGGCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGA‑AACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGa < 2:407932/288‑1 (MQ=255) ccGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAaga > 1:222650/1‑288 (MQ=255) cGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGTGACTGCAGAAGACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGtt < 2:313006/286‑1 (MQ=255) gTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCa < 1:659701/289‑1 (MQ=255) tGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATggggg > 1:378303/1‑269 (MQ=255) tGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATggggg < 2:378303/269‑1 (MQ=255) gCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGaa < 2:400627/175‑1 (MQ=255) gCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGaa > 1:400627/1‑175 (MQ=255) aCGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCCCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGtt > 1:49839/1‑193 (MQ=255) aCGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCCCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGtt < 2:49839/193‑1 (MQ=255) cTGCTCGGCGTGAAAGAGAACGTTATCGTGGGTCGTCTGAGCCCGGCAGGTACCGGTTACGCGGACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGaa < 1:103406/289‑1 (MQ=255) tGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAAtt > 2:14545/1‑289 (MQ=255) ccTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGaa < 2:107246/289‑1 (MQ=255) cTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTaa < 2:231097/187‑1 (MQ=255) gaACGTTATCGGGGGTCTTCTGATCCCGGCAGGTACCGGTTACGCGTACAACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCAt < 2:519172/287‑1 (MQ=255) aaCGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTccggcccggctgcaccgcaggtgactgcagaagacgcatctgccagcctggcagaactgctgaacgcaggtctgggcggttctgataacgagtaatcgttaatccgcaaataacgtaaaaacccgcttcggcgggtttttttatggggggagtttcgggaaagtgcatttgtcagaatatttaaggaatttctgaatactca > 1:408938‑M1/1‑87 (MQ=255) aaCGTGATCGTGGGTGGCCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAAt < 1:623100/289‑1 (MQ=255) aCGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAAt < 1:20521/288‑1 (MQ=255) aCGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAAt < 1:14545/287‑1 (MQ=255) aCGTTAGCGTGGGTCGTCTGAGCCCGGCAGGTACCGGTTACGCGTACAACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAGGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAACCCCGCTTCTGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGGATATTTAAGGAATTTCTGAATACTCATAAt < 2:638246/288‑1 (MQ=255) gTTATCGTGGGTCGTCTGATCCCTGCAGGTACCGGTTAGCCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGCTGCAGCTCCCCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAAt < 2:622768/287‑1 (MQ=255) gTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCAGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTagag < 1:453132/289‑1 (MQ=255) ggTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAAt < 2:799142/277‑1 (MQ=255) ggTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAAt > 1:423055/1‑277 (MQ=255) gtggacggATCGCGGCAGGTACAGGTGACGCGTACCACCATGAGCGTAGGCGGCGCCGGGCTGCGGCTGAAGCG‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGGAGAAGAGCGCATCTGCGAGCCGAGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGAAAGTAACGGAAAAACCCGCTTCGGCGGGTTTTGTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGGATTTCTGAATACTCATAATCAATGTAGAGAt < 2:37561/281‑1 (MQ=255) tcTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTccggcccggctgcaccgcaggtgactgcagaagacgcatctgccagcctggcagaactgctgaacgcaggtctgggcggttctgataacgagtaatcgttaatccgcaaataacgtaaaaacccgcttcggcgggtttttttatggggggagtttagggaaagagcatttgtcagaatatttaaggaatttctgaatactcataatcaatgtagagatg > 1:484783‑M1/1‑70 (MQ=255) tcTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCCCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGaa < 2:856436/255‑1 (MQ=255) tcTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCCCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGaa > 1:856436/1‑255 (MQ=255) tCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAatat > 1:844228/1‑288 (MQ=255) gCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCCCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGaa > 2:772069/1‑253 (MQ=255) gCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCCCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGaa < 1:772069/253‑1 (MQ=255) gCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAAt > 1:542903/1‑262 (MQ=255) gCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAAt < 2:542903/262‑1 (MQ=255) cAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGtt > 1:583457/1‑208 (MQ=255) cAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGtt < 2:583457/208‑1 (MQ=255) aCCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAactg > 1:152434/1‑287 (MQ=255) atcgaccggagcgtgtGCGTCGCCGTGCTGCGGGGGAAGGTccggcccggctgcaccgcaggttactgcagaagacgcagctgccagcctggcagaactgctgaacgcaggtctgggcggttctgataacgagtaatcgttaatccgcaaataacgtaaaaacccgcttcggcgggtttttttatggggggagtttagggaaagagcatttgtcagaatatttaaggaatttctgaatactcataatcaatgtagagatgactaatatcctgaaactgactgaactaa < 2:408938‑M1/281‑248 (MQ=255) caccaGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTccggcccggctgcaccgcaggtgactgcagaagacgcatctgccagcctggcagaactgctgaacgcaggtctgggcggttctgataacgagtaatcgttaatccgcaaataacgtaaaaacccgcttcggcgggtttttttatggggggagtttagggaaagagcatttgtcagaatatttaaggaatttctgaatactcataat > 1:460646‑M1/1‑39 (MQ=255) caccaGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTccggcccggctgcaccgcaggtgactgcagaagacgcatctgccagcctggcagaactgctgaacgcaggtctgggcggttctgataacgagtaatcgttaatccgcaaataacgtaaaaacccgcttcggcgggtttttttatggggggagtttagggaaagagcatttgtcagaatatttaaggaatttctgaatactcataat < 2:460646‑M1/245‑207 (MQ=255) tGCGTCGCCGTGCTGCGGGTGAAGCTCCCCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATTTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCGGCAAg > 1:242402/1‑289 (MQ=255) gCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGa < 1:655443/289‑1 (MQ=255) ccgTCGCCGTGCTGCGGGTGACGCTccggcccggctgcaccgcaggtgactgcagaagccgcatctgccagcctggcagaactgctgaacgcaggtcttggcggttctgataacgagtaatcgttaatccgcaaataacgtaaaaacccgcttcggcgggtttttttatggggggagtttagggaaagagcatttgtcagaatatttaaggaatttctgaatactcataatcaatgtagagatgactaatatcctgaaactgactgaactaattgagtcaaactcggca < 1:149971‑M1/288‑265 (MQ=255) gTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGAtt < 1:370931/289‑1 (MQ=255) gTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAAt > 2:36489/1‑228 (MQ=255) tCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGAtt < 2:222650/288‑1 (MQ=255) cGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCg > 1:836255/1‑289 (MQ=255) cGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGttttttt > 1:688417/1‑158 (MQ=255) ccGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGAt > 1:825589/1‑289 (MQ=255) cGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACg > 1:246380/1‑73 (MQ=255) cGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGACCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTc < 2:825589/288‑1 (MQ=255) gAAGCTccggcccggctgcaccgcaggtgactgcagaagacgcatctgccagcctggcagaactgctgaacgcaggtctgggcggttctgataacgagtaatcgttaatccgcaaataacgtaaaaacccgcttcggcgggtttttttatggggggagtttagggaaagagcatttgtcagaatatttaaggaatttctgaatactcataatcaatgtagagatgactaatatcctgaaactgactgaactaattgagtcaaactcggcaa < 1:217062‑M1/271‑266 (MQ=255) aGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTCCTGTGTaa < 1:774676/288‑1 (MQ=255) | TCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCT‑GGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCT‑‑CCGGCTGCACCGCAGGT‑‑‑‑‑‑‑‑‑‑GACTGCAGAAGA‑CGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTCCTGTGTAA > NC_000913/4189195‑4189766 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |