Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,538,206 G→A 2.6% R275H (CGC→CAC)  yhgF → putative transcriptional accessory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,538,2060GA 2.6% 559.7 / 2.8 157R275H (CGC→CAC) yhgFputative transcriptional accessory protein
Reads supporting (aligned to +/- strand):  ref base G (83/69);  new base A (2/2);  total (86/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.67e-01

Only 100 of 160 total aligned reads displayed.

GCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACGGTGGTGAGCGGTAAAGAAGAGGAAGGGGCGAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGAC  >  NC_000913/3537922‑3538491
                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                             
      ttATCTGTGGAGGACCGCGCATTTGGGTTCTACGGTGGTGAGCGGTAAAGAAGAGGAAGGGGCGAAATTCCGAGACTATTTCGATCAGCACGAACCGTTGTCCACGGTGCCTTCGCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCgtg                                                                                                                                                                                                                                                                                     <  2:414226/289‑1 (MQ=255)
      ttATCTGTGGAAGAACGCGCATTTGGTTTCTACGGTGGTGAGCGGTAAAGAAGAGGAAGGGGCGAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCgtg                                                                                                                                                                                                                                                                                     <  1:27885/289‑1 (MQ=255)
                aagaaCGCGCATTTGGTTTCTACGGTGGTGAGCGGTAAAGAAGAGGAAGGGGCGAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCCATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGCATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTTTGCTgg                                                                                                                                                                                                                                                                            >  1:864400/1‑288 (MQ=255)
                       cgcATTTGGTTTCTACGGTGGTGAGCGGTAAAGAAGAGGAAGGGGCGAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCCCCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGCGCACATAATCATGGATCACCTTGGCCTGCGCCTGAACTATGCCCCGGCGGATACCTGGCGCCAAGGCGTGGTGTGCTGGATGTGgc                                                                                                                                                                                                                                                                     >  2:483308/1‑288 (MQ=255)
                       cgcATTTGGTTTCTACGGTGGTGAGCGGTAAAGAAGAGGAAGGGGCGAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGgc                                                                                                                                                                                                                                                                     >  1:352178/1‑288 (MQ=255)
                       cgcATTTGGTTTCTACGGTGGTGAGCGGTAAAGAAGAGGAAGGGGCGAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGgc                                                                                                                                                                                                                                                                     >  2:314379/1‑288 (MQ=255)
                       cgcATTTGGTTTCTACGGTGGTGAGCGGTAAAGAAGAGGAAGGGGCGAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGgc                                                                                                                                                                                                                                                                     >  1:216845/1‑288 (MQ=255)
                            ttGGTTTCTACGGTGGTGGAGGGTAAAGAAGGGGAAGGGGCGAAATTCCGCGACTAATTCGGTCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGGAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCa                                                                                                                                                                                                                                                               <  1:916736/289‑1 (MQ=255)
                                   tgcgtgtgatgAGCGGAAAATAGGAGGAAGGGGCGCAATCCCGCGACTATTTCGGTCATCACGACCCGTTGTCCACGGTACCTTCTCACCGCGCGCTGGCGATGGTCCGTGGGCGTAACTAAGGCGAACTTCAGCTTGCGCCGAATGCCGATCCACAATCCGATGGGCCGCCCAAAGAGAGCTATTGCGAGCAAATCAGCATGGCTCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCt                                                                                                                                                                                                                                                        <  2:101934/284‑1 (MQ=255)
                                    tACGGTGGTGAGCGGTAAAGAAGAGGAAGGGGCGAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAACTCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCCCATCAAGGTGCTg                                                                                                                                                                                                                                                       >  1:195962/1‑289 (MQ=255)
                                      cGGTGGTGAGCGGTAAAGAAGAGGAAGGGGCGAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGTGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGa                                                                                                                                                                                                                                                      <  2:825326/288‑1 (MQ=255)
                                          ggtgAGCGGTAAAGAAGAGGAAGGGGCGAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCa                                                                                                                                                                                                                                                  >  2:446121/1‑288 (MQ=255)
                                              aGCGGTAAAGAAGAGGAAGGGGCGAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCCTCTgg                                                                                                                                                                                                                                             >  1:137991/1‑289 (MQ=255)
                                              aGCGGTAAAGAAGAGGAAGGGGCGAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTgg                                                                                                                                                                                                                                             >  2:110984/1‑289 (MQ=255)
                                                   tAAAGAAGAGGAAGGGGCGAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAAcc                                                                                                                                                                                                                                        >  1:109432/1‑289 (MQ=255)
                                                      agaagaGGAAGGGGCGAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGaa                                                                                                                                                                                                                                     <  1:446121/289‑1 (MQ=255)
                                                             gacggggCTAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCGCCGCGCGCTGGCGTTGTTCCGTGGGCGTAACGAAGGCGAACTTCAGCTTTCGCTGAATGCCGCACCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGATGTGGCGCAGTAAGGTGCTGATGCATCTGGAAACCGAACTGATgg                                                                                                                                                                                                                              >  2:71291/4‑289 (MQ=255)
                                                             gAAGGGGCGAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATgg                                                                                                                                                                                                                              >  2:59587/1‑289 (MQ=255)
                                                             gAAGGGGCGAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATgg                                                                                                                                                                                                                              >  1:613441/1‑289 (MQ=255)
                                                             gAAGGGGCGAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATgg                                                                                                                                                                                                                              >  1:521543/1‑289 (MQ=255)
                                                             gAAGGGGCGAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATgg                                                                                                                                                                                                                              >  1:883205/1‑289 (MQ=255)
                                                                ggggCGAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGc                                                                                                                                                                                                                            >  1:157066/1‑288 (MQ=255)
                                                                  ggCGAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCAcc                                                                                                                                                                                                                         >  1:267893/1‑289 (MQ=255)
                                                                   gCGAAATTCCGCGACTATTTCGATCATCACGAAACGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCAcc                                                                                                                                                                                                                         <  1:255701/288‑1 (MQ=255)
                                                                    cGAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGt                                                                                                                                                                                                                       >  1:563613/1‑289 (MQ=255)
                                                                            cgcgACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGCTCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTTGCGCACAGGCGTGGTGAGCTGGACGTGGCGCACTCAGGTGATGACGCATCCGGAAACGGTACTGATGGGCACTGTgcgctacc                                                                                                                                                                                                               >  2:740636/1‑285 (MQ=255)
                                                                            cgcgACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAAc                                                                                                                                                                                                               <  1:314379/289‑1 (MQ=255)
                                                                                     ttCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAg                                                                                                                                                                                                      >  1:755057/1‑289 (MQ=255)
                                                                                         gtcatcaCGAACCGTTGTCCACGGTGCCTTCTTACCGCGGGCGGGCGATGTTCCGTGGGCGTAACGAAGGCGGACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATg                                                                                                                                                                                                   <  2:339349/287‑1 (MQ=255)
                                                                                                  aaCCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCa                                                                                                                                                                                          >  1:265126/1‑288 (MQ=255)
                                                                                                       ttGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCt                                                                                                                                                                                    >  1:239947/1‑289 (MQ=255)
                                                                                                             aCGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGccc                                                                                                                                                                              <  1:521484/289‑1 (MQ=255)
                                                                                                             aCGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGccc                                                                                                                                                                              <  1:298821/289‑1 (MQ=255)
                                                                                                               ggTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCt                                                                                                                                                                                                                                                                              <  1:916153/191‑1 (MQ=255)
                                                                                                                 tGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTa                                                                                                                                                                           >  2:263200/1‑288 (MQ=255)
                                                                                                                   ccTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCACAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTaa                                                                                                                                                                          >  2:895573/1‑287 (MQ=255)
                                                                                                                           ccGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGGGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGccggg                                                                                                                                                                 >  2:682525/1‑284 (MQ=255)
                                                                                                                           ccGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATAAAGGTGCTGATGCATCTGGACACCGAACTGATGGGCACCGTGCGCGCACGTGCGGACGATGAAGCAATCAACGTCTTTGCCCGTAAGCTGCAAGAt                                                                                                                                                                >  2:263797/1‑289 (MQ=255)
                                                                                                                           ccGCGCGCTGGCCATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGCGAGCTATTGCGAGCAAATCATCATGGCTCACCTTGGCCTGCGCCTCAACAATGCCCCAGCGGATAGCTGCCCCACAGGCGTCGTCAGTCGGCCGTCGCCCACCACGGCGCTGCTTCACCGGGCACCCTCACCGATGGGCACGCTGCCCGCACGTCCGGAAACACATGCAATCAGCCTCTTTTCCCGTCAccctcccca                                                                                                                                                                 >  2:438563/1‑281 (MQ=255)
                                                                                                                             gcgcgcTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGa                                                                                                                                                                 >  1:149322/1‑286 (MQ=255)
                                                                                                                               gcgcTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCACAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATct                                                                                                                                                              >  1:235924/1‑287 (MQ=255)
                                                                                                                                      cGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGaa                                                                                                                                                                                                                                     >  2:144159/1‑209 (MQ=255)
                                                                                                                                      cGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGaa                                                                                                                                                                                                                                     <  1:144159/209‑1 (MQ=255)
                                                                                                                                         tGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATggcggc                                                                                                                                                  <  1:682525/289‑1 (MQ=255)
                                                                                                                                         tGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATggcggc                                                                                                                                                  <  2:149322/289‑1 (MQ=255)
                                                                                                                                             ccGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATggcggc                                                                                                                                                  >  2:916862/1‑285 (MQ=255)
                                                                                                                                                tGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGcc                                                                                                                                           >  1:296165/1‑289 (MQ=255)
                                                                                                                                                    catAACGAAGGCGTACTTCAGCTTTCGCGGAAGGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCACAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGa                                                                                                                                        <  2:235924/286‑1 (MQ=255)
                                                                                                                                                    cGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTTGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGa                                                                                                                                        <  1:482600/288‑1 (MQ=255)
                                                                                                                                                    cGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGa                                                                                                                                        <  1:470699/288‑1 (MQ=255)
                                                                                                                                                    cGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGa                                                                                                                                        <  1:617956/288‑1 (MQ=255)
                                                                                                                                                     gTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACt                                                                                                                                      <  1:63861/289‑1 (MQ=255)
                                                                                                                                                     gTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCACAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACt                                                                                                                                      <  1:895573/289‑1 (MQ=255)
                                                                                                                                                      tAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGaa                                                                                                                                                                                                                                     <  2:476300/193‑1 (MQ=255)
                                                                                                                                                             ggCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACt                                                                                                                                      <  1:93697/281‑1 (MQ=255)
                                                                                                                                                               cGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGaa                                                                                                                                                                                                                                     <  2:284191/184‑1 (MQ=255)
                                                                                                                                                                 tACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACt                                                                                                                                      <  1:498866/277‑1 (MQ=255)
                                                                                                                                                                  acttccgctgtgcctgaagcgcGATCCACAGTTCGATGAACCGCCCAAAGAGAGCTAGTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTCGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATg                                                                                                                         <  2:563613/268‑1 (MQ=255)
                                                                                                                                                                         agcaggCTGAATGCCGCTCCACAGTTCGAGGGGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGTATCACCTTGGCCGGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCt                                                                                                                    <  2:409091/282‑1 (MQ=255)
                                                                                                                                                                          tttCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCt                                                                                                                    <  1:597532/286‑1 (MQ=255)
                                                                                                                                                                            tCGCTGAATGCCGTTCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATc                                                                                                               <  1:415115/289‑1 (MQ=255)
                                                                                                                                                                              gCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACTTGGCGCATCAAGGTGCTGATGCATCTGGCAACCGAACTGATGGGCCCCCTTCGCGAACGTGCGGAAGATGAAGCAATCCACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCAGCCCCTTCCggcattcggtccacggagggccccgaacac                                                                                                             >  2:32339/1‑261 (MQ=255)
                                                                                                                                                                                         aTCCACATTTCGATGGGCCGCCCAAAGAGGGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAAGGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTAc                                                                                                  <  2:883205/289‑1 (MQ=255)
                                                                                                                                                                                         aTCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTcctggc                                                                                                    >  1:409091/1‑282 (MQ=255)
                                                                                                                                                                                          tCCACAGTTCTATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACt                                                                                                 <  1:703056/289‑1 (MQ=255)
                                                                                                                                                                                          tCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTTGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTAc                                                                                                  <  1:147483/288‑1 (MQ=255)
                                                                                                                                                                                               ggctCGATGGGCCGCCCAAAGAGAGCTATTGCGCGCCAAGCATCATGGATCACCTTGGCCCGCGCCTGACCAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGGGAGCTGGACGTGCGGCATCAAGGTGCTGATGCACCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGt                                                                                            <  2:188383/286‑1 (MQ=255)
                                                                                                                                                                                                 ttCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTaa                                                                                          >  1:104910/1‑289 (MQ=255)
                                                                                                                                                                                                   cGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCt                                                                                                                                                                       <  2:773963/210‑1 (MQ=255)
                                                                                                                                                                                                   cGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTaaaa                                                                                        >  2:225181/1‑289 (MQ=255)
                                                                                                                                                                                                     aTGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGt                                                                                      >  1:473394/1‑289 (MQ=255)
                                                                                                                                                                                                      tGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTg                                                                                     >  1:512952/1‑289 (MQ=255)
                                                                                                                                                                                                           cgccCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGGACTGGGGCAAAAGTGGCggt                                                                                >  1:824350/1‑289 (MQ=255)
                                                                                                                                                                                                           cgccCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGACCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCggt                                                                                >  2:348959/1‑289 (MQ=255)
                                                                                                                                                                                                                        cgaTTGCCAGCAAATCATCATGGCTCACCGTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTTGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCAc                                                                    <  2:265126/286‑1 (MQ=255)
                                                                                                                                                                                                                        cTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGGTAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCACCGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGcc                                                                      >  2:624761/1‑286 (MQ=255)
                                                                                                                                                                                                                        cTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGcc                                                                      >  1:372983/1‑286 (MQ=255)
                                                                                                                                                                                                                        cTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGAAGGCGGCCCCTGCCGGACt                                                                                                                                      >  2:628368/1‑222 (MQ=255)
                                                                                                                                                                                                                            tGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCa                                                               >  1:919377/1‑289 (MQ=255)
                                                                                                                                                                                                                              cGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCaa                                                              >  1:348154/1‑288 (MQ=255)
                                                                                                                                                                                                                               gAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCaaa                                                             <  1:263200/288‑1 (MQ=255)
                                                                                                                                                                                                                                aGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATg                                                                                                                                                                                                   >  2:266375/1‑153 (MQ=255)
                                                                                                                                                                                                                                     atcatcATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGCTCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCCCTGGCCAACtggtg                                                       >  2:466886/1‑288 (MQ=255)
                                                                                                                                                                                                                                             ggATCACCTGGGGCTGCGCCCGAACAAGGCCCGGGGGGGTAGCTGGCGGAAGGGGGTGGGGGGCTGGACGGGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAGCGGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCGTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGGTGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGGGGcgaccga                                               <  2:521543/288‑1 (MQ=255)
                                                                                                                                                                                                                                               aTCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCt                                                                                                                    <  2:755121/217‑1 (MQ=255)
                                                                                                                                                                                                                                               aTCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCt                                                                                                                    >  1:755121/1‑217 (MQ=255)
                                                                                                                                                                                                                                                    cTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCAttt                                       >  1:180862/1‑289 (MQ=255)
                                                                                                                                                                                                                                                      tGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGGGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAg                                                                                                                                                                                                <  2:516950/134‑1 (MQ=255)
                                                                                                                                                                                                                                                        gCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCAtt                                        >  2:590367/1‑284 (MQ=255)
                                                                                                                                                                                                                                                        gCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCAtt                                        <  1:590367/284‑1 (MQ=255)
                                                                                                                                                                                                                                                         ccTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTAccc                                   <  1:808195/288‑1 (MQ=255)
                                                                                                                                                                                                                                                             cgcCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCTGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCCGCGTACTGTGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGcac                               >  1:361442/1‑288 (MQ=255)
                                                                                                                                                                                                                                                             cgcCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAATGATGGGCCTCGTTCCGGGTCTGCGTACTGGGGTAAACGTGGCCGTGGTCGATGCCACTGGCCAACGGGTGGCGTCCGACACCCTTTACCCGcac                               >  2:347482/1‑288 (MQ=255)
                                                                                                                                                                                                                                                             cgcCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGcac                               >  2:724961/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                        tGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGCCGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGCGCCTCGATCCGCGTCTGCGTCCTGGGGTAAACGTGGCGGTGGTCGATGCCACTGGCATACTGGCCGCCACCGACCCCATTTAGCCGCACACCCGACAgcc                    >  2:215969/1‑286 (MQ=255)
                                                                                                                                                                                                                                                                        tGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGc                    >  2:167373/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                             cggcggATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCACTTACCCGCACACCGGACAGGCTGCaaa              >  1:554005/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                cggATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTAc                                                                                                  <  1:639389/202‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       cgggCGCAAAGTCGTGGGGAGCTGGATGGGGGGCATCAAGGTGCTGATGCAGCTGTAAACCGAACTGATGGCCACCGTGCGCGAACGTGCGGAGGATGAAGCAATCAACTGCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGAt     <  2:824350/286‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         ggCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGAc  <  1:724961/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                             
GCGTGATTATCTGTGGAAGAACGCGCATTTGGTTTCTACGGTGGTGAGCGGTAAAGAAGAGGAAGGGGCGAAATTCCGCGACTATTTCGATCATCACGAACCGTTGTCCACGGTGCCTTCTCACCGCGCGCTGGCGATGTTCCGTGGGCGTAACGAAGGCGTACTTCAGCTTTCGCTGAATGCCGATCCACAGTTCGATGAGCCGCCCAAAGAGAGCTATTGCGAGCAAATCATCATGGATCACCTTGGCCTGCGCCTGAACAATGCCCCGGCGGATAGCTGGCGCAAAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGAC  >  NC_000913/3537922‑3538491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: