Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,147,185 A→T 3.7% T17S (ACT→TCT)  frwD → putative enzyme IIB component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,147,1850AT 3.7% 397.0 / 4.4 111T17S (ACT→TCT) frwDputative enzyme IIB component of PTS
Reads supporting (aligned to +/- strand):  ref base A (53/51);  new base T (2/2);  total (58/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01

Only 100 of 111 total aligned reads displayed.

CTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTA  >  NC_000913/4146899‑4147467
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                          
cTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACt                                                                                                                                                                                                                                                                                          >  1:718621/1‑289 (MQ=255)
   aTCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTtat                                                                                                                                                                                                                                                                                       >  1:617107/1‑289 (MQ=255)
   aTCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAAAGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTtat                                                                                                                                                                                                                                                                                       >  1:175942/1‑289 (MQ=255)
   aTCCATGGTTTCACGCTCAGCCCGGAGAATATGCAGCAGAGGCTGGATGTACTGATCCCGCCGCATATCAGTCAGATCGATCTGTTACCGCCTCGTCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAACACCTGGTCGATGAAGGAGGTGCCTGCGCCGCCGCCTGCCGATGTGGCATCGTTGCGCGAAATGGCCGAAGCGGCCGGACTTCAGGTTACCGTGGCTGGCTCAAATGGCATACCTGGCGGCAGCAACCGCCCGCGTAGCCGTCGCGCCGCATGCtatg                                                                                                                                                                                                                                                                                       >  2:174620/1‑286 (MQ=255)
               aCGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAGGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGCAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGa                                                                                                                                                                                                                                                                            >  1:634750/1‑288 (MQ=255)
                     aGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTg                                                                                                                                                                                                                                                                     >  1:216790/1‑289 (MQ=255)
                        cGGGAGAATATGCAGCAGGGGCTGGATGGACTGATCCCGCTGAATATCAGGCAGATCCATTTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGaa                                                                                                                                                                                                                                                                  <  1:477581/289‑1 (MQ=255)
                               atatGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAACAGTtgt                                                                                                                                                                                                                                                           >  1:115738/1‑289 (MQ=255)
                               atatGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTtgt                                                                                                                                                                                                                                                           <  2:617107/289‑1 (MQ=255)
                                tatGCAGCAGGCGGTGGATGTACTGATCCCGCGGAATATCGGGACGATCCATCTGTTAACGTTTCAGCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTtgtg                                                                                                                                                                                                                                                          <  2:713923/289‑1 (MQ=255)
                                tatGCAGCACGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTtgtg                                                                                                                                                                                                                                                          <  1:776153/289‑1 (MQ=255)
                                  tGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGcc                                                                                                                                                                                                                                                        <  2:589674/289‑1 (MQ=255)
                                     agcagGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCAGGAAAAGTTGTGccgtg                                                                                                                                                                                                                                                     >  2:269218/1‑286 (MQ=255)
                                      gcagGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTCAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTTTGCCTggt                                                                                                                                                                                                                                                    >  2:559086/1‑287 (MQ=255)
                                       cagGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTa                                                                                                                                                                                                                                                   >  1:195015/1‑289 (MQ=255)
                                        agGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTag                                                                                                                                                                                                                                                  >  1:223017/1‑289 (MQ=255)
                                         ggCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACATGGTGGCAGTAACCGCCTGCGTAAGCCGCGTGGCACATCATGCGATGGCGGCGGAACGGCTGGCACAGTTGTGCCCGTTaga                                                                                                                                                                                                                                                 >  2:893483/1‑289 (MQ=255)
                                          gcgcTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTagag                                                                                                                                                                                                                                                >  2:111215/1‑289 (MQ=255)
                                                          tCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCa                                                                                                                                                                                                                                >  1:510681/1‑289 (MQ=255)
                                                              gCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGa                                                                                                                                                                                                                            >  1:77697/1‑289 (MQ=255)
                                                                      tCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAgg                                                                                                                                                                                                                    <  2:755110/289‑1 (MQ=255)
                                                                       cAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAGCCGAAATACCGCCTGCTGGGGAAAACATGGACGAGGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAggg                                                                                                                                                                                                                   <  2:303662/289‑1 (MQ=255)
                                                                         ggCAAATCCAGCTGTTACCATTTCATCAGTACGGCGAACCCAAATACCGCCTGCTGGGGAAAACATGGTCGAGGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAg                                                                                                                                                                                                                                  <  1:901431/272‑1 (MQ=255)
                                                                              aTCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCCTTGAAACTCAGGGCGCGCTg                                                                                                                                                                                                            >  1:482803/1‑289 (MQ=255)
                                                                              aTCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCAtt                                                                                                                                                                                                                              >  1:925310/1‑271 (MQ=255)
                                                                               tCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCt                                                                                                                                                                                                             <  2:223017/287‑1 (MQ=255)
                                                                                 caaccggtACCGGTTCATCAGCTCGGGGGACCTGAATTCCGGCGGCTGGGGGAAAGATGGTAGATGAAAGAGGGGGCCGTGACCTTGGTCAGTGAGTGGGCAAAGATGCGCCGAACGGGAGAACGGGCCGGACGTTAGGGTACCGGTGGGGGTTAAAAAGGGATACCTGGTGGCAGTAAACCCCTTCGTTAACGGCGTGGCGGCTAACTATATGGGGGCGGAACGGGTTGAAAAGGTGGTGCTGTTAGCGAAGGGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGa                                                                                                                                                                                                         <  2:527020/282‑1 (MQ=255)
                                                                                   tCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATATCTGGTGGCAGTAACCGCCTGCGTAAGCGGCTTGGCGCATACTTATATGGTGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGCAAGGTTGGGCGTCGGAATTGGAAcccctgtctctcttacaac                                                                                                                                                                                                       >  2:901431/1‑271 (MQ=255)
                                                                                         aCCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGaa                                                                                                                                                                                                 >  1:599424/1‑289 (MQ=255)
                                                                                                aagcaGTACGGCGAAACGAAAGACCGCCTGCTGGGGAAAACATGGTCGATGAAATAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTa                                                                                                                                                                                          <  1:591818/286‑1 (MQ=255)
                                                                                                     gTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATTCTTATATGGCGGCGGAACGGCTGGAAAAGTtgtg                                                                                                                                                                                                                                                          <  1:518559/220‑1 (MQ=255)
                                                                                                     gTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATTCTTATATGGCGGCGGAACGGCTGGAAAAGTtgtg                                                                                                                                                                                                                                                          <  1:518569/220‑1 (MQ=255)
                                                                                                     gTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATTCTTATATGGCGGCGGAACGGCTGGAAAAGTtgtg                                                                                                                                                                                                                                                          >  2:518559/1‑220 (MQ=255)
                                                                                                     gTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATTCTTATATGGCGGCGGAACGGCTGGAAAAGTtgtg                                                                                                                                                                                                                                                          >  2:518569/1‑220 (MQ=255)
                                                                                                       aCGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCgg                                                                                                                                                                                      <  1:261580/286‑1 (MQ=255)
                                                                                                         ggCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAg                                                                                                                                                                                 >  2:731948/1‑289 (MQ=255)
                                                                                                           cGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGa                                                                                                                                                                               >  2:279229/1‑289 (MQ=255)
                                                                                                           cGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGa                                                                                                                                                                               >  1:804387/1‑289 (MQ=255)
                                                                                                                  aaaTACCGGCTGCTGGGGAAAACATGGTCGATGAAAGAGGGGCCTGCGCCGTCGTCAGGTGGTGTGGCAACGGTGGGCGAAATGGCAGAACGGGCCGGAATTCAGATGACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGGGGCGCATACGTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAGGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATccg                                                                                                                                                                         <  2:634750/288‑1 (MQ=255)
                                                                                                                  aaaTACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCGGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATccgc                                                                                                                                                                        <  2:510681/289‑1 (MQ=255)
                                                                                                                       ccGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTTCCTGTTAGCGAAGTGGGGAGTCAGCATAGAAACTCAGGGCGCGCTGGGAACGGAGAAGCGTCTAGCGGATGAGGATATCCGCCTGGc                                                                                                                                                                   >  1:879936/1‑289 (MQ=255)
                                                                                                                         gCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGGCTGTTAGAGAAGTGGGGGGCCAGCATTGACACTGAGGGCGCGCTGGGAACGGAGAACCGTTTAGAGGAGGAGGATATCTGCCGGGCgg                                                                                                                                                                 >  2:713463/1‑289 (MQ=255)
                                                                                                                           ctgctgGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGa                                                                                                                                                                <  1:111215/288‑1 (MQ=255)
                                                                                                                            tgctgGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATg                                                                                                                                                              <  2:195015/289‑1 (MQ=255)
                                                                                                                            tgctgGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGGTAAACTGGCCTACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCTGAACGGTTGGAAACGTAGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGACACCCAGGGCGTGCTGGGAACGGTGATTCGTTTTGCGGATGAGGGTATCGGCCGGGCGGacg                                                                                                                                                              >  2:853127/1‑287 (MQ=255)
                                                                                                                                ggggAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTg                                                                                                                                                           <  2:599424/288‑1 (MQ=255)
                                                                                                                                  ggAAAACATGGTCGATGAAAAGGGGGCCGGGGCCGGCGTCAGCGTATGTGGCAACGATGCGGGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGct                                                                                                                                                         <  2:873702/288‑1 (MQ=255)
                                                                                                                                     aaaCATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTg                                                                                                                                                      <  1:209546/288‑1 (MQ=255)
                                                                                                                                         aTGGTGGATGAAAGAGGTGCGTGTGCCGTCGTCAGCGGATGTGGACACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTAGATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGAt                                                                                                                                                 <  2:493371/289‑1 (MQ=255)
                                                                                                                                           ggTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTc                                                                                                                                                                                                                                    >  1:378935/1‑204 (MQ=255)
                                                                                                                                                aTGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGCGAAGTGGGGCGTCAGCATTGCAACTCAGGGCGCGCTGGGAACGGCGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGat                                                                                                                                          >  1:364193/1‑289 (MQ=255)
                                                                                                                                                           gCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGCTGTTGCTCTGTTGATTACGGATATCGAGCTTg                                                                                                                                >  1:470898/1‑288 (MQ=255)
                                                                                                                                                           gCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTg                                                                                                                                >  1:908372/1‑288 (MQ=255)
                                                                                                                                                           gCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGc                                                                                                                               <  1:317194/289‑1 (MQ=255)
                                                                                                                                                            ccTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGcc                                                                                                                              >  1:168297/1‑289 (MQ=255)
                                                                                                                                                                     gtcgtcAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGa                                                                                                                      <  1:731948/289‑1 (MQ=255)
                                                                                                                                                                      tcgtcAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGa                                                                                                                      <  2:216790/288‑1 (MQ=255)
                                                                                                                                                                                atatgGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGaa                                                                                                            <  1:174620/285‑1 (MQ=255)
                                                                                                                                                                                agtgagCACCGATGTGCGAAATGGCAGAACGGGCCTGACTTCAGGTTACCGTGGGAGGTTAAAATGGAGTACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGaa                                                                                                            <  2:718621/283‑1 (MQ=255)
                                                                                                                                                                                aTGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAAc                                                                                                           >  1:662551/1‑289 (MQ=255)
                                                                                                                                                                                aTGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGACGGGCGGTTGTTGCCCTGTTGATTACGGATTTCGAGCTTCCCTGTTGCGAGCGCTTTGaaa                                                                                                           >  1:497563/1‑288 (MQ=255)
                                                                                                                                                                                  gtgGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACAt                                                                                                         <  1:269218/289‑1 (MQ=255)
                                                                                                                                                                                    ggCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTg                                                                                                       <  1:279229/289‑1 (MQ=255)
                                                                                                                                                                                     gCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTg                                                                                                       >  1:745880/1‑288 (MQ=255)
                                                                                                                                                                                       aaCGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCg                                                                                                    <  2:745880/289‑1 (MQ=255)
                                                                                                                                                                                          gATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGata                                                                                                                                         <  2:347616/249‑1 (MQ=255)
                                                                                                                                                                                          gATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGata                                                                                                                                         >  1:347616/1‑249 (MQ=255)
                                                                                                                                                                                               gcgAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTAtgtg                                                                                             <  2:908372/288‑1 (MQ=255)
                                                                                                                                                                                                             cGGGCCGGACTTAAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGGGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTAc                                                                              <  1:369062/289‑1 (MQ=255)
                                                                                                                                                                                                                       ttCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATAc                                                                                                                                                                                                                                                                                           <  2:772213/74‑1 (MQ=255)
                                                                                                                                                                                                                       ttCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATAc                                                                                                                                                                                                                                                                                           >  1:772213/1‑74 (MQ=255)
                                                                                                                                                                                                                         cAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGACTCGTTTAGCGGATGAGGACATCCGCCGGGCGGATGTTGCTCTGTTAATTACGGATATCGAGCTTGCCGGTTCCGACCGATCTGCATATTGGCGCTCAGTGCAATTGCGCACCTACTCATTCCTTCGt                                                                  >  2:602819/1‑289 (MQ=255)
                                                                                                                                                                                                                          aGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACCTTGCCGCTATGTGCAATGCAGCATCTACGGATTCCTGCGtt                                                                 >  2:657042/1‑288 (MQ=255)
                                                                                                                                                                                                                             ttACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAg                                                               <  1:56851/288‑1 (MQ=255)
                                                                                                                                                                                                                               aCCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATTTACGCATTTCTGCGTGAGcc                                                             >  1:691135/1‑288 (MQ=255)
                                                                                                                                                                                                                               aCCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATACGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATTGACTATCTACGCATTCCTGCGTGTGcc                                                             >  2:485783/1‑288 (MQ=255)
                                                                                                                                                                                                                                ccGTGGGAGGTTAAAAGGGCATACCTGGGGGCAGTAAACGCCTGCGTAAGCAGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGGAAAGTGGTGCCTGTTGGAGAAGTAGGGAGGCAGCATTGAAACTCAGGGCGCACTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGGTTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGc                                                           <  2:482803/289‑1 (MQ=255)
                                                                                                                                                                                                                                 cGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCa                                                          >  2:673730/1‑289 (MQ=255)
                                                                                                                                                                                                                                  gTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAg                                                         <  2:662551/289‑1 (MQ=255)
                                                                                                                                                                                                                                         gTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTaa                                                  >  1:472086/1‑289 (MQ=255)
                                                                                                                                                                                                                                          ttAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAg                                                         <  1:827915/281‑1 (MQ=255)
                                                                                                                                                                                                                                          ttAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAAt                                                 >  1:853484/1‑289 (MQ=255)
                                                                                                                                                                                                                                                tGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAgcgc                                           <  2:522738/289‑1 (MQ=255)
                                                                                                                                                                                                                                                tGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAgcgc                                           <  2:804387/289‑1 (MQ=255)
                                                                                                                                                                                                                                                   cATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTgg                                                                                                                                                                                                           <  2:635163/126‑1 (MQ=255)
                                                                                                                                                                                                                                                   cATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTgg                                                                                                                                                                                                           >  1:635163/1‑126 (MQ=255)
                                                                                                                                                                                                                                                    aTACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTg                                       >  2:698871/1‑289 (MQ=255)
                                                                                                                                                                                                                                                    aTACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCGGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGTATCTACCCAGTCCCGCGTGAGCTGCAGCGGGTAATGAgctgtc                                         >  2:827915/1‑283 (MQ=255)
                                                                                                                                                                                                                                                        cTGGTGGCAGTAACCGCCGGCTGAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGGGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCa                                   <  2:811453/289‑1 (MQ=255)
                                                                                                                                                                                                                                                            tggCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGt                               >  1:726787/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                  tAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCtt                           >  1:628306/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                        ccTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGc                                                                    >  2:798440/1‑240 (MQ=255)
                                                                                                                                                                                                                                                                        ccTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGc                                                                    <  1:798440/240‑1 (MQ=255)
                                                                                                                                                                                                                                                                                aGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCaa            >  1:430413/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                     cgTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGc                                                           <  1:15839/236‑1 (MQ=255)
                                                                                                                                                                                                                                                                                      gTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATc     <  1:922534/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       tGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATc     <  1:485783/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       tGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCGGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATc     <  2:77697/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        ggCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATATACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCt    <  1:853127/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                          cgcATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTa  <  2:245593/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                          
CTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTTGTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGATGAGGATATCCGCCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTA  >  NC_000913/4146899‑4147467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: