New junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 4094337 = | NA (NA) | 23 (0.530) +ACAAGTGCCTTATCCGGCCTACA |
7/238 | 4.8 | NA | noncoding (23/402 nt) | REP299 (repetitive extragenic palindromic) element, contains 9 REP sequences | REP299 (repetitive extragenic palindromic) element, contains 9 REP sequences |
? | NC_000913 | = 4094681 | NA (NA) | noncoding (367/402 nt) | REP299 (repetitive extragenic palindromic) element, contains 9 REP sequences | REP299 (repetitive extragenic palindromic) element, contains 9 REP sequences | |||||
Rejected: Coverage evenness skew score above cutoff. |
CCCAGCCTTTCAGCCAGGCGTCGGTGGTGGCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGG > NC_000913/4094196‑4094478 | cccAGGCTGTCAGGTAGGGGTCGGTGGTGGCTTTGATCATTCCGTGGACATACGTGGACTGGGTAATGCTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCgcgc < 2:59616/143‑1 (MQ=255) cccAGCCTTTCAGCCAGGCGTCGGTGGTGGCTTTGATCATTCCCTGGACAAACCAGGACTGTGTATTGTTTTTCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCgcgc < 1:223775/143‑1 (MQ=255) cccAGACATCCAGCCAGGCGTCGGTGGTGGCTTTGATCATTCCAGGGACAAACCAGGACGGAGTAATGTTTGGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGACGCACGACCCGTAGGCCGGATAAGGCGCTCgcgc < 1:832858/143‑1 (MQ=255) cAGCCTTTCAGCCAGGCGTCGGTGGTGGCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTCGGCCGCCACATTCGCTCGcgccg > 2:537333/1‑143 (MQ=255) cAGCCTTTCAGCCAGGCGTCGGTGGTGGCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTTGcgccg > 1:877157/1‑143 (MQ=255) cAGCCTTTCAGCCAGGCGTCGGTGGTGGCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGcgccg > 1:90940/1‑143 (MQ=255) gcaggcaggCGTCGGTGGGGGCTTTGTTCATTCCCTGGACAACCGAGGGCTGCGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCgg < 2:633443/142‑1 (MQ=38) ccAGGCGTCGGTGGTGGCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATGAGGCGCTCGCCCCGCGTCCGGcagt > 1:51928/1‑141 (MQ=255) aGGCGTCGGTGGTGGCTTTGATAATTACCTGGACAAACGAGGAGTGAGTAATGTTTTGCATGTTATGTGTTCCTGTAAATTTGGTGTTGTTGGATGGACGACCTGTAGGGCGGATAAGAGGGTCGTGACGCATCCGGgaatga > 2:195232/1‑141 (MQ=255) ggccgcGGTGGTGGCATGGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGcaatcaa < 1:436823/138‑1 (MQ=255) tCGGTGGTGGCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGcc > 1:446135/1‑143 (MQ=255) gCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGAc > 1:650549/1‑143 (MQ=255) tttGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTTAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGccgacggcgacgc > 2:582425/1‑132 (MQ=255) cATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCCTCCGGCAATCAATGCCTGATGCGACGCTGCcgc > 2:20561/1‑143 (MQ=255) aTTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCGCGCGGCGAATCCGGCAATGAATGACTGATGCGACGCTGTcgcg > 1:326996/1‑143 (MQ=255) ccTGGACAAACCAGGACTGAGTAAGGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCtt < 1:333533/143‑1 (MQ=255) cTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTACTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCCCGCGCCGCGACCGCCACGCAACGCCTGATGgggcgcgggcgccggggc > 2:907360/1‑137 (MQ=255) gACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCa > 2:176965/1‑143 (MQ=255) gACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCa > 1:148180/1‑143 (MQ=255) caCCAGGACTGAGTAAGGTTTGGCATGTTCTGGGTTCCTGTATATTCGCTGTTGTCGGCTGGGCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGcc < 2:223126/142‑1 (MQ=21) cgaGGACTGAGTAATGTGTGGCAGGTGCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTa < 1:216693/141‑1 (MQ=255) cAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACTTGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCCACCAATGCCTGGTGCGACGCTGTCGCCGCTTATCAGGCCtcc > 2:789420/1‑141 (MQ=38) ggACGGGGTAATGTTTTGCATGTTCTGTGTTCCTGGAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACaa < 2:605973/143‑1 (MQ=255) gACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATACGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTCTCAGGCCTACAAc > 1:139169/1‑143 (MQ=255) tGAGTTGTGTTTCGCATGTGCTGTGTTCCGGTAAATGCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTAt < 2:49266/143‑1 (MQ=21) tGAGTAATGTTTTGCATGTTCTGTGTTCCTGTGAATTCGGTGTTGTCGGATGCACGACCAGTAGGCCGGATAAGCCGCTCGTGCCTCATCCGGCAATCAATGCCTGATTCGGCGCTGTCGCGTCTTATCAGGCTGACGACTAt > 1:515555/1‑143 (MQ=21) tGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTCTCacggctccacatat > 2:609723/1‑130 (MQ=37) gTTGTGTGTTCGTGTGAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATGAGGCGCTCGCGCCGGATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCt < 2:296161/143‑1 (MQ=18) gtgtctcggTAAATGCGGTGTTGTCGTACGCACGGCCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATaa < 1:179770/135‑1 (MQ=18) ctgcgccTTTAAGTGCGGGGCTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCACTGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATaa < 2:332403/138‑1 (MQ=17) tgtTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCACTCCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATacg > 1:841705/1‑141 (MQ=255) tgtTCCTGTAAATTCGATGTTCTCGGATGCACCATGCGCAGGCCGGATAGGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCCTATCAGGCCTACCACTATTGCCGCCCTGTCGGCCTCAGAAg > 2:406826/1‑143 (MQ=17) gtTCCTGTAAATTCGGCGTTGTGAGAGGCGCGACCCGTAGGCCGGATAAGGCGCGCGCGCCGCATCCGGCAATCAATGCCGGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAgg < 1:738162/143‑1 (MQ=17) ttCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGc > 1:365676/1‑143 (MQ=255) ccTGTAAATTCGCTGTGGTCCGATGCACGACCCGTAGGCCGGATAAGTCGCTCGCTCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGTCCTATAACTATTGCCGACCTGTAGGCCTGATAAGGCAc < 2:760692/143‑1 (MQ=18) cTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACCAATATTGCCGACCGGTAGGGCTGATAAGGCaac > 1:103962/1‑141 (MQ=255) tAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTtgt > 1:245906/1‑143 (MQ=255) aTTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCCGCCGGTCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATACGGCACTCGGGcc > 2:541064/1‑143 (MQ=18) tgcGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCg < 1:830733/141‑1 (MQ=255) tgtCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCTCGCCGCATCCGGCAATCAATGCCCGCGGCGACGCTGTCGCGCCTTGACCGGCCTACCACTGTGGCCGGCCAGTGGGCCCGATACAGCCCTGGTGCCGCAggcggc > 2:196074/1‑143 (MQ=12) tgtCGGAGGAAGGACCCGTAGGGCGGATAAGGCGCCCGGGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTCTTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGc < 1:490397/143‑1 (MQ=14) acGGAAGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAAGCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGcaa < 1:113761/142‑1 (MQ=255) cGGGTGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGAGGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGcaat < 1:851887/143‑1 (MQ=255) gagtgccggcctgTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTCTCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGcaatca < 2:318476/131‑1 (MQ=1) gATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCCCATCCGGcaagca > 1:462436/1‑143 (MQ=255) aTGCAGGACCCGTAGGCCGGGTAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGcaatcaa < 2:497278/143‑1 (MQ=255) aCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTGTTAGCAGGCCTACAACTATTGCCGAGCTGTAGGCGTGATAGGGCACGTGTGTCGGATGCGGCaatgaatggg > 2:819184/1‑141 (MQ=14) aCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGcc > 1:567898/1‑143 (MQ=255) aCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGCCCTACAACTATTGCCGACCTGTCGGCCTGATAAGGCACTTGTGCCGCATCCGCCAATCAATGcc > 1:841517/1‑143 (MQ=255) gACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGGATCACGCAATCAATGCATg > 2:150080/1‑143 (MQ=255) cggcggCCGGAGAAGGGGCTCGCGGCGCGTCCGGCAATCAATGCCTGAGGCGACGCTGTGGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGc < 2:184351/139‑1 (MQ=14) cGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGc > 1:55990/1‑143 (MQ=255) cGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGc > 2:718643/1‑143 (MQ=37) gTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTATGCCTGATAAGGCACTTGTGCCGCATCCGGCAACGCATGCCTGATGCg > 1:874488/1‑143 (MQ=255) gTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGCTAAGGCACTTGTGCCGCATCCGGCAATCAATGCCAGATGCg > 1:66183/1‑143 (MQ=255) gCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGc > 1:282153/1‑143 (MQ=255) cGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGCTAAGGCGCTCGTGCCGCCTCCGGCCATCACTGCCCGCTGCCACGCTg > 2:304110/1‑143 (MQ=14) gATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTCCCGACCTGTAGGCCTGATAAGGCACCCGTGCCGCATCCGGCAATCACTGCCTGATCCGACGccctc > 2:194991/1‑139 (MQ=14) aTAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCGATCAATGCCTGATGCGACGCTGTcg > 1:908355/1‑143 (MQ=255) gagggCGCCCGCGCCGCATCCCGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcgc < 2:148180/140‑1 (MQ=255) ggCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCGATACCTGGTGccaacatgcaccgtc > 1:654713/1‑129 (MQ=14) tCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGc > 1:827/1‑68 (MQ=37) cgccgcATCCGGCAATCAATGCCTGATGCGACGCGGCCGCGCCCTATAAGGCCTACACCTATTGCCGACCTGTCGCCCTCAGAACGCACCCGCCCCGCATCCCGCACCCACTACCTGAGGCGCCGCCGTcgccccctcccata > 2:365098/1‑132 (MQ=2) | CCCAGCCTTTCAGCCAGGCGTCGGTGGTGGCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGG > NC_000913/4094196‑4094478 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
Reads not counted as support for junction |
read_name Not counted due to insufficient overlap past the breakpoint. |
read_name Not counted due to not crossing MOB target site duplication. |