Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,726,390 G→T R123L (CGC→CTC)  nemR → transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,726,3900GT95.7% 119.8 / NA 46R123L (CGC→CTC) nemRtranscriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (24/20);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGC  >  NC_000913/1726251‑1726529
                                                                                                                                           |                                                                                                                                           
tCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAg                                                                                                                                          >  1:473453/1‑143 (MQ=255)
      gAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCCGTGGTTGCCGGGCGTCAAGACGCTCTGCCGGAGTGTGCGTTCCGTCCGAAGATATGCTCAGCGCGaa                                                                                                                                    >  1:659840/1‑142 (MQ=255)
          gcaccgcccGCGACCGCATACTGGCTTGTTACCAGCAAACGCTGAACCAGTTTTGCCAACATGGAACCATGAGTGGTTTCCTGACAGTAAAACTCTCTGCCGAAGGGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGAt                                                                                                                                <  2:840202/136‑1 (MQ=255)
          gTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATggct                                                                                                                                >  1:570586/1‑141 (MQ=255)
                cccGCGACCGCATACTGGCTTATTACCAGCAAACCCTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTGGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGt                                                                                                                          <  1:870531/142‑1 (MQ=255)
                  cgcgACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTGGCCTGACAGTAACACTCTCTGCCGAAGTGTGCGCTCTGTCAGACGATATGCTCAGCGCGCCGGTTAAAGGTGc                                                                                                                        >  2:128559/1‑143 (MQ=255)
                   gcgACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACAATCAGTGGTTGACTGACAGTAAAACTCTCTGCCGAAGTGTGCGCTCTGTCCGAAGATATGCTCAGCGCGATGGATAAAGGTGcc                                                                                                                       >  2:399003/1‑142 (MQ=255)
                     gACCGTATACTTGCTTATTACCATCAAACACTGAACCAGTTCTGCCAACGTGTAACCATCAGTAGTTTCCTTACAGTAAAACTCTCTACCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTcg                                                                                                                     <  2:9285/143‑1 (MQ=255)
                              cTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGGGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGGTAAAGGTGCCCGCGGCGGGAt                                                                                                            >  2:348333/1‑143 (MQ=255)
                               tGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATc                                                                                                           >  1:767762/1‑143 (MQ=255)
                                 gcctattaCCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGc                                                                                                         <  1:506103/140‑1 (MQ=255)
                                  cTTATGACCAGCAAACACTGGACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGcc                                                                                                        <  2:487078/143‑1 (MQ=255)
                                     attaCCAGCAAGCGCTGGACCAGTTTTGCCAACGTGGAACCATCAGTGGTGGCCTGACAGTAAAACTCTCTTCCGAAGTGTGCGAGCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTg                                                                                                     <  2:584877/143‑1 (MQ=255)
                                       taCCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGct                                                                                                   <  1:388091/143‑1 (MQ=255)
                                         ccAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGctct                                                                                                 >  1:689440/1‑143 (MQ=255)
                                          cAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAATAAAACTCTCTGCCGAAGTGTGCGATCTGTCGGAAGATATGCTCAGCGCGACGGAGAAAGGGGCTCCCGGCGGGATCGCCCGGctcgc                                                                                                >  2:314872/1‑141 (MQ=255)
                                                   cTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGGTCTCTCAGGCGCt                                                                                       >  1:485585/1‑143 (MQ=255)
                                                     gAACCAGTTCTGCCACCATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTgg                                                                                     <  1:120946/143‑1 (MQ=255)
                                                       aCCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGaa                                                                                   >  1:43952/1‑143 (MQ=255)
                                                        ccAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGaaa                                                                                  <  1:827062/143‑1 (MQ=255)
                                                            ttgtGCCCACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATgg                                                                              <  1:765968/140‑1 (MQ=255)
                                                                gCCAAGCTGGAACCATCAGTGGTTGCCTGACAGTAAACCTCTCTGCCGGAGTGTGCGATCTGTCAGCAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGt                                                                          <  2:800800/143‑1 (MQ=255)
                                                                  cAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAGATGGCCgggg                                                                        >  2:850321/1‑140 (MQ=255)
                                                                  cAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTga                                                                        >  1:653885/1‑143 (MQ=255)
                                                                  cAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATACTCAGCGCGATGGATAAAGATGCTCGCGGCGTTATCGCCCTGCTCTCTCAAGCGCTGGAAAATGGCCGTga                                                                        >  2:73329/1‑143 (MQ=255)
                                                                    aCATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTGAGCGCGATGGGTAAAGGGGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCGGGAAAATGGCCGTgaga                                                                      >  1:496681/1‑143 (MQ=255)
                                                                     taTGGAACCGTGAGTGGTGGCCGGAACTTAAAACTCTCTGTCGAAGTGTGCGATCTGTCAGAAGATATGCTCCGCGCGATGGATAAAGGTGGTCGAGGCGTGATCGCCTTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGaa                                                                     <  2:365729/142‑1 (MQ=255)
                                                                     cATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGaa                                                                     <  1:804852/143‑1 (MQ=255)
                                                                         gAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCAt                                                                 >  2:417241/1‑143 (MQ=255)
                                                                         gAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTACGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCGCAGGCGCTGGAAAATGGCCgcgccagccat                                                                 >  1:69458/1‑133 (MQ=255)
                                                                          gaCCGTCAGTGGTTGCCTGACAGTAAAACGCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCAtt                                                                <  2:899443/142‑1 (MQ=255)
                                                                           agcATCAGTGGTTGCCTGACCGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTg                                                               <  1:641370/141‑1 (MQ=255)
                                                                              aTCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGACAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGttt                                                            <  2:347373/143‑1 (MQ=255)
                                                                               tCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTa                                                           >  1:600651/1‑143 (MQ=255)
                                                                                           tGACAGTAAAACTCTCTGCCGACGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAAGCGCGGGAAAATGGCTGTGAGACCCATAGTTTGACCTTTtttgtc                                               >  2:545302/1‑141 (MQ=255)
                                                                                           tGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTCCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGCCCGTGAGAACCATTGTTTAACCTTTtgtgcc                                               >  2:545303/1‑141 (MQ=255)
                                                                                           ggACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGc                                               <  1:430225/142‑1 (MQ=255)
                                                                                                 tAAAACTCTCTGCAGAAGTGTGCGATCTGTCAGCAGATATGCTCAGCGCGATAGATAAAGGTGCACGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAACATGGCCGTGCGAACCATAGTTTAACTTTTCGTGACGAACCg                                         >  2:248807/1‑143 (MQ=255)
                                                                                                  aaaaCTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGc                                        >  1:769960/1‑143 (MQ=255)
                                                                                                                       cgctCTGTCAGAAGCGATGCGCAGCGCGATGGATTCAGGTGCTCGCGGCGTGCTCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTATCCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCttt                   <  2:621095/140‑1 (MQ=255)
                                                                                                                       cGATCTGTCAGAAGATATGCTCAGCGCGCTGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCCCTCTCAGGCGCTGGCAACTGGCCGTGAGGACCATTGTTCAACGCTTTGTGGCGCACCCGCGCAACGGGCACaaacgccat                   >  2:39815/1‑136 (MQ=255)
                                                                                                                        gatgcgTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTa                  <  2:570586/138‑1 (MQ=255)
                                                                                                                              tCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTCACCTTTTGTGGCGAACCGCTGCACAAGGAAAAAATGCATTaagaaat            >  1:686751/1‑137 (MQ=255)
                                                                                                                              tCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTCTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTGACGCACt            >  2:433280/1‑143 (MQ=255)
                                                                                                                                      tatGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTg    <  1:861980/143‑1 (MQ=255)
                                                                                                                                        tGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGgc  <  2:612989/143‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGC  >  NC_000913/1726251‑1726529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: