| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,726,390 | G→T | R123L (CGC→CTC) | nemR → | transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,726,390 | 0 | G | T | 95.7% | 119.8 / NA | 46 | R123L (CGC→CTC) | nemR | transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (24/20); total (25/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGC > NC_000913/1726251‑1726529 | tCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAg > 1:473453/1‑143 (MQ=255) gAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCCGTGGTTGCCGGGCGTCAAGACGCTCTGCCGGAGTGTGCGTTCCGTCCGAAGATATGCTCAGCGCGaa > 1:659840/1‑142 (MQ=255) gcaccgcccGCGACCGCATACTGGCTTGTTACCAGCAAACGCTGAACCAGTTTTGCCAACATGGAACCATGAGTGGTTTCCTGACAGTAAAACTCTCTGCCGAAGGGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGAt < 2:840202/136‑1 (MQ=255) gTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATggct > 1:570586/1‑141 (MQ=255) cccGCGACCGCATACTGGCTTATTACCAGCAAACCCTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTGGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGt < 1:870531/142‑1 (MQ=255) cgcgACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTGGCCTGACAGTAACACTCTCTGCCGAAGTGTGCGCTCTGTCAGACGATATGCTCAGCGCGCCGGTTAAAGGTGc > 2:128559/1‑143 (MQ=255) gcgACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACAATCAGTGGTTGACTGACAGTAAAACTCTCTGCCGAAGTGTGCGCTCTGTCCGAAGATATGCTCAGCGCGATGGATAAAGGTGcc > 2:399003/1‑142 (MQ=255) gACCGTATACTTGCTTATTACCATCAAACACTGAACCAGTTCTGCCAACGTGTAACCATCAGTAGTTTCCTTACAGTAAAACTCTCTACCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTcg < 2:9285/143‑1 (MQ=255) cTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGGGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGGTAAAGGTGCCCGCGGCGGGAt > 2:348333/1‑143 (MQ=255) tGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATc > 1:767762/1‑143 (MQ=255) gcctattaCCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGc < 1:506103/140‑1 (MQ=255) cTTATGACCAGCAAACACTGGACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGcc < 2:487078/143‑1 (MQ=255) attaCCAGCAAGCGCTGGACCAGTTTTGCCAACGTGGAACCATCAGTGGTGGCCTGACAGTAAAACTCTCTTCCGAAGTGTGCGAGCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTg < 2:584877/143‑1 (MQ=255) taCCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGct < 1:388091/143‑1 (MQ=255) ccAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGctct > 1:689440/1‑143 (MQ=255) cAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAATAAAACTCTCTGCCGAAGTGTGCGATCTGTCGGAAGATATGCTCAGCGCGACGGAGAAAGGGGCTCCCGGCGGGATCGCCCGGctcgc > 2:314872/1‑141 (MQ=255) cTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGGTCTCTCAGGCGCt > 1:485585/1‑143 (MQ=255) gAACCAGTTCTGCCACCATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTgg < 1:120946/143‑1 (MQ=255) aCCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGaa > 1:43952/1‑143 (MQ=255) ccAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGaaa < 1:827062/143‑1 (MQ=255) ttgtGCCCACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATgg < 1:765968/140‑1 (MQ=255) gCCAAGCTGGAACCATCAGTGGTTGCCTGACAGTAAACCTCTCTGCCGGAGTGTGCGATCTGTCAGCAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGt < 2:800800/143‑1 (MQ=255) cAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAGATGGCCgggg > 2:850321/1‑140 (MQ=255) cAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTga > 1:653885/1‑143 (MQ=255) cAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATACTCAGCGCGATGGATAAAGATGCTCGCGGCGTTATCGCCCTGCTCTCTCAAGCGCTGGAAAATGGCCGTga > 2:73329/1‑143 (MQ=255) aCATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTGAGCGCGATGGGTAAAGGGGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCGGGAAAATGGCCGTgaga > 1:496681/1‑143 (MQ=255) taTGGAACCGTGAGTGGTGGCCGGAACTTAAAACTCTCTGTCGAAGTGTGCGATCTGTCAGAAGATATGCTCCGCGCGATGGATAAAGGTGGTCGAGGCGTGATCGCCTTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGaa < 2:365729/142‑1 (MQ=255) cATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGaa < 1:804852/143‑1 (MQ=255) gAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCAt > 2:417241/1‑143 (MQ=255) gAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTACGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCGCAGGCGCTGGAAAATGGCCgcgccagccat > 1:69458/1‑133 (MQ=255) gaCCGTCAGTGGTTGCCTGACAGTAAAACGCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCAtt < 2:899443/142‑1 (MQ=255) agcATCAGTGGTTGCCTGACCGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTg < 1:641370/141‑1 (MQ=255) aTCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGACAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGttt < 2:347373/143‑1 (MQ=255) tCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTa > 1:600651/1‑143 (MQ=255) tGACAGTAAAACTCTCTGCCGACGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAAGCGCGGGAAAATGGCTGTGAGACCCATAGTTTGACCTTTtttgtc > 2:545302/1‑141 (MQ=255) tGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTCCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGCCCGTGAGAACCATTGTTTAACCTTTtgtgcc > 2:545303/1‑141 (MQ=255) ggACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGc < 1:430225/142‑1 (MQ=255) tAAAACTCTCTGCAGAAGTGTGCGATCTGTCAGCAGATATGCTCAGCGCGATAGATAAAGGTGCACGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAACATGGCCGTGCGAACCATAGTTTAACTTTTCGTGACGAACCg > 2:248807/1‑143 (MQ=255) aaaaCTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGc > 1:769960/1‑143 (MQ=255) cgctCTGTCAGAAGCGATGCGCAGCGCGATGGATTCAGGTGCTCGCGGCGTGCTCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTATCCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCttt < 2:621095/140‑1 (MQ=255) cGATCTGTCAGAAGATATGCTCAGCGCGCTGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCCCTCTCAGGCGCTGGCAACTGGCCGTGAGGACCATTGTTCAACGCTTTGTGGCGCACCCGCGCAACGGGCACaaacgccat > 2:39815/1‑136 (MQ=255) gatgcgTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTa < 2:570586/138‑1 (MQ=255) tCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTCACCTTTTGTGGCGAACCGCTGCACAAGGAAAAAATGCATTaagaaat > 1:686751/1‑137 (MQ=255) tCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTCTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTGACGCACt > 2:433280/1‑143 (MQ=255) tatGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTg < 1:861980/143‑1 (MQ=255) tGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGgc < 2:612989/143‑1 (MQ=255) | TCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGC > NC_000913/1726251‑1726529 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |