Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,374	G→A	G226D (GGC→GAC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,374	0	G	A	97.7%	120.5 / NA	43	G226D (GGC→GAC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (20/22);  total (20/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGC  >  NC_000913/1756235‑1756506                                                                                                                                            |                                                                                                                                     gttGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCt                                                                                                                                   <  2:338535/142‑1 (MQ=255)          gAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACtt                                                                                                                          >  2:905762/1‑143 (MQ=255)            aCAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAACATCGAAAACCGGGAAGACCTCAACAACttgg                                                                                                                        >  1:674382/1‑141 (MQ=255)            aCAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTcg                                                                                                                        <  2:284006/143‑1 (MQ=255)                    tAGTCTTTGTTGCTGTTTTCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGGGAGCAGCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATc                                                                                                                <  1:750403/143‑1 (MQ=255)                             ttgCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGCCGGCGAAAACATCCACATCATCTCCAAAATCGCAAACCAGGAAGACCCCAACAACTCCGACGAACTCCTCGAAGcc                                                                                                       >  2:461130/1‑143 (MQ=255)                                  gctTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGa                                                                                                  >  1:775384/1‑143 (MQ=255)                                    ttccttcgcccGTAAGCCTTCGGCCGTTATCGAAATCCGTGTGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAACCCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACg                                                                                                <  2:459852/133‑1 (MQ=255)                                    ttCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGACAACCAGGCAGACCTCGATAACTTCGACGAAATCCTCGAAGCCTCTCACg                                                                                                >  1:243348/1‑143 (MQ=255)                                        catATTCGTAAGCGTTGTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGcat                                                                                            >  2:197016/3‑143 (MQ=255)                                                   gCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTgcgc                                                                                 >  1:476039/1‑143 (MQ=255)                                                               tATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTgg                                                                     <  1:82813/143‑1 (MQ=255)                                                               tATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTATGACGGCATCATGGTTGCGCGTGGCGACCggg                                                                     >  2:81462/1‑143 (MQ=255)                                                                  cGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAAACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGgtg                                                                  >  1:657097/1‑143 (MQ=255)                                                                       tCCGTTTGCTCCTGAAAGCGCACGGCAGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGaa                                                             <  2:439076/143‑1 (MQ=255)                                                                            gAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATccc                                                        <  1:428884/143‑1 (MQ=255)                                                                             cgtacgtggaaGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCGCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCg                                                       <  1:849674/134‑1 (MQ=255)                                                                             aGCACCTGCAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCg                                                       <  1:332030/143‑1 (MQ=255)                                                                              gCACCTGAAAGCGCTCGGCGGCGAAAACATCCACACCATCTCCAAACTCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCgg                                                      <  2:347297/143‑1 (MQ=255)                                                                               cACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGt                                                     <  1:531421/143‑1 (MQ=255)                                                                                aCCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTa                                                    <  1:905762/143‑1 (MQ=255)                                                                                  cTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTaga                                                  <  1:174376/143‑1 (MQ=255)                                                                                  cTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGCAGAAATCCCGGTaga                                                  >  2:522528/1‑143 (MQ=255)                                                                                   tGAAAGCGCACGACACCGAAAACATCCACATAATCTCCAAACTCGAAAACGAAGAAGAACTCAACAACTTCGACGAAAACCTCGAAGCCTCTGACGGCATCATGGTTGAGCGTAGCGATCTGGGTGTGGaacacacgactgac                                                 >  2:77319/1‑131 (MQ=255)                                                                                    gAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTggacg                                                >  1:14125/1‑141 (MQ=255)                                                                                      aaGCTCACGGCGGCGAAAACAGCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACGTCGACGAAATCCGCGAAGCCTCTGACGGCATCAGGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAgaagaa                                              <  2:701012/143‑1 (MQ=255)                                                                                      aaGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAgaagaa                                              <  1:465290/143‑1 (MQ=255)                                                                                       aGCCCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGaagaag                                             <  1:577341/143‑1 (MQ=255)                                                                                           cACGGCGGCGAAAACATCCACAGCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTCGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTAt                                         <  1:712148/143‑1 (MQ=255)                                                                                                 ggcgAAAACATCCACATCATCTCCAAAATCGAAAACCAGTAAGACCAAAACGTCTTCGATGTTATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGc                                   <  2:784678/143‑1 (MQ=255)                                                                                                  gcgAAAACATCCACATCACCTCCATAATCGAACACCCGGAAGACCTCGACAACTTCGACGAAATCCTCGAAGCCTCGGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGcc                                  <  1:333267/143‑1 (MQ=255)                                                                                                  gcgAAAACAGCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGcc                                  <  1:326693/143‑1 (MQ=255)                                                                                                   cgAAAACATCCTCATCATCACCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATACTTGCCCGTGGCAACCTCGGTGTACAAATCCAAGTCGACGACGTTATCTCCGccc                                 >  2:177436/1‑143 (MQ=255)                                                                                                    gAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAGTCCGGGTAGAAGAAGTTATCTTGGCGCa                                >  1:284948/1‑143 (MQ=255)                                                                                                         cATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCagaaga                           <  1:146067/143‑1 (MQ=255)                                                                                                               catcctCTCCAAAATCGAAAACCAGGAAGACCTCAACAACATCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAgatgatga                     <  1:369174/143‑1 (MQ=255)                                                                                                                   atcTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAACCCCGGTAGCAGAAGTCATCTTCGCCCAGAACATGATGATCGa                 >  2:807467/1‑143 (MQ=255)                                                                                                                      tcCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTTTCTTCGCCCAGAAGATGATGATAGaaaa              >  1:355700/1‑143 (MQ=255)                                                                                                                       ccAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTGGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGaaaaa             <  1:650050/143‑1 (MQ=255)                                                                                                                          aaaTCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGt          >  1:578668/1‑143 (MQ=255)                                                                                                                             tCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTCGAAATCCCGGTCGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATc       >  1:36200/1‑143 (MQ=255)                                                                                                                                aaaaCCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAATCCTCTGACGGCATGATGGTAGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGACGAAGTTATGTTCGCCGAGAAGCGGATGACCGGAAACTGTAGCCGt    >  1:142928/1‑143 (MQ=255)                                                                                                                                  aaCCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAACATGTATCCGTGc  >  2:852/1‑143 (MQ=255)                                                                                                                                            |                                                                                                                                     TTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGC  >  NC_000913/1756235‑1756506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑