Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,132,7870AC100.0% 147.0 / NA 51I204S (ATC→AGC) wcaAputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (23/28);  total (23/28)

TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132650‑2132925
                                                                                                                                         |                                                                                                                                          
gcgggTAGAGGGTAAACAGCTGATATCTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTGGCGGTAAAAGTGGAGGGGCCCGGAGAATGTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                       <  1:643436/140‑1 (MQ=255)
     tAGAGGGTTAACAGCTGATATTTTGTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                  <  1:437136/143‑1 (MQ=255)
          ggTAGACAGCTGATACTTTTTGTTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt                                                                                                                             <  2:698306/143‑1 (MQ=255)
          ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt                                                                                                                             >  1:813416/1‑143 (MQ=255)
           gTAAACGGCTTATAGTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAATGTGTAAGTACTCTGTGTATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttc                                                                                                                            <  2:373083/143‑1 (MQ=255)
                cAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACtt                                                                                                                       <  1:788753/143‑1 (MQ=255)
                    tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTACTGGGTGACGAGGCGCTCTGCATTGCACCGTGATTGAGATGGAGGCTCTGCGTCGCCTCTTCTACTTACCa                                                                                                                   >  2:882135/1‑143 (MQ=255)
                      ttatTTTTCGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGCGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACg                                                                                                                 <  1:681839/142‑1 (MQ=255)
                                  ggCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTTATCTGCATTTCACCGTGATTGAGATGCAGGCTCTGCGTCGCCTCTCGAGCTTTCCAGGGGTCGCCGGACt                                                                                                     >  2:512090/1‑143 (MQ=255)
                                       ggTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacc                                                                                                <  1:491003/143‑1 (MQ=255)
                                        gTCGAATTTGTCTTTGTGCTCGCGGTAAAAGTGGAAGTACCCGGAGCATTTTTTCTGGGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacca                                                                                               <  2:315033/143‑1 (MQ=255)
                                          cGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATCTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCCACTTTCCAAGATTCTCCGTACTccaccacc                                                                                             >  2:694403/1‑143 (MQ=255)
                                              tttGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATcc                                                                                         >  2:175560/1‑143 (MQ=255)
                                                  tCTTTGTGCTTGCTGTAAAAGTGGAAGGTCCCGGAGAGCGTCTTCTTTGTTGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAg                                                                                     <  2:638784/143‑1 (MQ=255)
                                                        tgCTTGCGGTAAAAGTGGACGTACCGGGAGAATTTTGGCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGGTCGCCGTACTCCACCACAATCCGCAGGAAggt                                                                               >  1:816151/1‑141 (MQ=255)
                                                        tgCTTGCGGTAAAAGTGGAAGTACCCGGAAAATTTTTTCGGTGACGAGGTGATCAGCATTTGACCGTGGTTGATATGCAGACTCTGCGTCGCCTCTTCTACTTTCCACGGAACGCAGTACTCCACCACCATCCGCAGGAAgct                                                                               >  2:126225/1‑141 (MQ=255)
                                                          cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTCCTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaacacgt                                                                             >  2:28969/1‑138 (MQ=255)
                                                          cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGt                                                                             <  1:629848/143‑1 (MQ=255)
                                                           ttGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCGCGGTTCGCCGTACTCCACCACCATCCGCAgcgagttttc                                                                            >  1:50174/1‑134 (MQ=255)
                                                           ttGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc                                                                            >  1:490100/1‑143 (MQ=255)
                                                               ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACGATCCGCAGGAAGATGCCGTaa                                                                        >  2:729789/1‑143 (MQ=255)
                                                                   aaggtcgAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTTTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCt                                                                    <  1:404062/137‑1 (MQ=255)
                                                                   aaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCGCTTCTCCTTTCCCCGGTGCGCCGTCCTCCACCACCATCCGCAGCAAGATGTCGCAATCCt                                                                    >  2:679657/1‑143 (MQ=255)
                                                                      tttgaatttctctgagaATTTTTTCGGTGACGAGGTGATCTGCAGGACACCGTGAGCGATATGCAGGCCCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                                 <  1:729768/130‑1 (MQ=255)
                                                                        tgAAATACCCGGAGAATCATTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGcc                                                               <  1:431873/142‑1 (MQ=255)
                                                                        ggAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGTCTCTTCTACTGTCCACGGTTCGCCGGACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGcc                                                               <  1:362439/143‑1 (MQ=255)
                                                                          aaGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGc                                                             <  1:92755/143‑1 (MQ=255)
                                                                            gTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCACATGTCGTACTCCTGTGCCGCtt                                                           >  2:858661/1‑143 (MQ=255)
                                                                              aCCCGGAGAATTTTTTTGGTGACGAGGTAATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTTGGTGTACTCCACCACCATCCGTATCAAGATTTTGTAATCCTTCGCCGCTTTc                                                         >  2:6089/1‑143 (MQ=255)
                                                                                ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTCCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAg                                                       <  1:779480/143‑1 (MQ=255)
                                                                                   gagaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTc                                                    <  2:748613/143‑1 (MQ=255)
                                                                                     gaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCgg                                                  >  1:106374/1‑143 (MQ=255)
                                                                                     gaATTTTTGCGGTGAGGGGGTGATCTGCATTTCGCCGTGATTGGTATGCAGGCTCTGCGTCGCCTCTTCTCCTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCgg                                                  <  2:178875/143‑1 (MQ=255)
                                                                                        ttttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGGCGCTTTCAGTTCGGTaa                                               >  1:257266/1‑142 (MQ=255)
                                                                                         tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc                                              <  1:673314/143‑1 (MQ=255)
                                                                                          ttttCGGTGACGAGGTGATCTGCATTTCACCGTGACTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCCCGGTTCGCCGTACTCCACCACCATCCTCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTCCGGTATCg                                             >  1:320370/1‑143 (MQ=255)
                                                                                            ttCGGGGACGAGGTGATCTGCATTTCACCGTGATTGAGATGCAGGCTCTGCGTCGCCTCTTCTACTGTCCACGGGTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa                                           <  2:280467/143‑1 (MQ=255)
                                                                                                gTGGCGAGGTGATGTCCTTTCCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa                                       <  2:825545/143‑1 (MQ=255)
                                                                                                gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGACGCTCTCACTTCGGTATCGaacaaa                                       >  2:24565/1‑143 (MQ=255)
                                                                                                  gACGAGGTGCTCTGCACGTCACCGTGATTGATATGCAGGCTCTGTGATACCTCGTCTACTTACCGCGGCTTTCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca                                     <  1:169326/143‑1 (MQ=255)
                                                                                                       ggtggtCTGCAGTGCTCCGTGATTGATATGCAGGCTCGGCGCCGCCTCTTCTACTTTGCACGGTTTGCCGTGCGCGACCACCATCCGCAGTAAGGTGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCtt                                <  2:668084/143‑1 (MQ=255)
                                                                                                       ggTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCACGAAGATGTCGTAATCCTGTGCCCCTTTCAGTTCGGTATCGAACAAACACCCtt                                >  2:592727/1‑143 (MQ=255)
                                                                                                                    tccccGTGATTGCTATGCTGGCTCTGCGTCGCCTCTTCTACTTTCCTCGGGTCGCTGTACTCCACCTCGATCTGCATTACGATGTCGTAATCCTTTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGc                   <  2:495325/140‑1 (MQ=255)
                                                                                                                    tCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGc                   <  2:329300/143‑1 (MQ=255)
                                                                                                                        cGTGATTGATGTGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAg               <  2:813416/143‑1 (MQ=255)
                                                                                                                        cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCGGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAGACGCCATGCCCAg               >  1:81521/1‑143 (MQ=255)
                                                                                                                        cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCAACACCATCCGGGGGGAGATGTCGTAATCCTGTGCCGCTTTTCGTACGGTATCGAACAAACACTATTTCAACCGACATGtcccg               >  2:439331/1‑141 (MQ=255)
                                                                                                                          cggtcgccaggCAGGCTCGGCGCCGCCGCTCCTACTGTCCACTGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGt             <  2:557318/133‑1 (MQ=255)
                                                                                                                              tgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCAACACCATCCACAGGACGCTGTCGTACTCCTCTGCCGCTTTCAGTTCGGTATCGACAAAACACTCTTTGACCCGCCATCCCCAGCGAAAg         >  2:710183/1‑143 (MQ=255)
                                                                                                                                  atGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGAACAGAccc     >  1:115928/1‑142 (MQ=255)
                                                                                                                                     cAGGCTCTGCGTCGCCTCTGCGACGTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGACTGCTTACGCCTGTGCCGCTTTCAGCTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGt  <  1:209324/143‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132650‑2132925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: