| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 418,961 | Δ1 bp | coding (1073/1296 nt) | phoR → | sensory histidine kinase in two‑component regulatory system with PhoB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 418,960 | 0 | G | . | 97.7% | 179.4 / ‑3.2 | 43 | coding (1072/1296 nt) | phoR | sensory histidine kinase in two‑component regulatory system with PhoB |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base . (25/17); total (25/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACG‑CATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAATCATCACGAAAGT > NC_000913/418818‑419097 | ggTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTGCGAACCCGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACG‑CATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAACg < 1:221425/143‑1 (MQ=255) tCTGGCAACGAAGATCGGCTACGCAGTGCGATTTCGCACCTGGTCTATACCGCCGTGACTGCAACGCTGGAAGGCACG‑CATATCACCGTACCCTGGCAGGGAGTGCTGATCGGTGCCGAATTTAGCGTTGA‑AGATAACgaccg < 2:617199/143‑5 (MQ=255) tGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGCAGGCACG‑CATATCACCGTACGCTGGCAGCGAGCGCCGCACGGTGCCGAATTTAGCGGTGA‑AGATGACgtccggg > 2:212094/1‑137 (MQ=255) tGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCAAGCCCAATTAGAG‑ATTGTTGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCggg > 1:927819/1‑143 (MQ=255) ggCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACG‑CATATCACCGTACGCTGGCAGCGAGTGCCGCACGGGGACGAAGTTAGCGTTGA‑AGATAAC‑GACCggga > 2:305381/1‑142 (MQ=255) gAAGATCAGCTCCGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACG‑CATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATGTAGCGTTGA‑AGATAAC‑GACCGGGCACTGCa > 2:250711/1‑143 (MQ=255) gAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACG‑CATATCACCGTACGCTGGCAGCGAGTGCGGCACGGTGCCGAATTTAGCGTTGA‑AGATGAC‑GACCGGGCATTGCa > 1:80808/1‑143 (MQ=255) tCATCTACTGAGAGCGAGTACGAACCTGGTCTATACCGCCGTGAATCATACGCCGGAAGGCACG‑CAGATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGa < 2:669226/143‑1 (MQ=255) gCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACG‑CATATCACCGTACGCTGGCAGCGAGAGCCGAACGGTGCCGAACTTAGCGTGGA‑AGCAAAC‑GACCGGGCAATGCACCGGAGCAGATTc > 2:878584/1‑143 (MQ=255) tttCGAAGCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACG‑CATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGa < 1:200531/143‑1 (MQ=255) tttCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACG‑CATCTCACCGTACGCTGGCAGCGCGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCCTCGCACCCGCGCCTCTCCCGCGCCtca > 2:919675/1‑141 (MQ=255) tttCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACG‑CATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGGGCATATTCCGCGCCTGa > 1:391813/1‑143 (MQ=255) ttCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACG‑CATATCACCGTACGCTGGCAGCGAGTGCCGCACGGGGCCGAATTTAGCGTTGA‑CGATAAC‑GACCGGGCGTCGCACCGGGGCATATTCCgcgcatcag > 1:913262/1‑138 (MQ=255) gAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACG‑CATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGa < 2:919437/143‑1 (MQ=255) gAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACG‑CATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGa > 1:243838/1‑143 (MQ=255) tGGTCTATAACGCAGTGAATCATACGCCGGAAGGCACG‑CATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGtt < 2:773151/143‑1 (MQ=255) cTATAACGCCGTGAATCATACGCCGGAAGGCACG‑CATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTa < 1:618197/143‑1 (MQ=255) tataACGCCGTGAATCATACGCCGGAAGGCACG‑CATATCACCGTACGCTGCCGACGAGTGCCACACGGCGCAGTTGTTAGCGTTGA‑AGATCAC‑GCCCGGGCATTGCACCGGCGCATCTTCCGCGCCTTGCCGAGCGtttctct > 2:329196/1‑139 (MQ=255) ataACGCCGTGAATCATACGCCGTATTGCACG‑CATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATGTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAACATATTCCGCGCCTGACCGAGCGCTTTTATc > 2:116240/1‑143 (MQ=255) taACGCCGTGGATCATAGGGCGGAAGGAACG‑CATACCACCGTACGGTGGCAGCGAGTGCAGCTCGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGACCATATTCCGCGCCTGACCGAGCGTTTTTATcg < 2:505448/143‑1 (MQ=255) gaaCGCCGTGAGTCATACGCCGGAAGGCACG‑CAGATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATcg < 2:585731/142‑1 (MQ=255) aaCGCCGTGAATCATACGCCGGAAGGCACG‑AGCACAGGCGGACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATcgc < 1:723494/143‑1 (MQ=255) gCCTTGAATCATACGCCGCAATGCACG‑CATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGtt < 1:25390/143‑1 (MQ=255) gCCGTGAATCATACGCCGGAAGGCACG‑CATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGtt < 1:860344/143‑1 (MQ=255) gCCGTGAATCAGACGCCGGAAGGCACG‑CATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGtt < 1:247136/143‑1 (MQ=255) ccGTGAATCCTACGCCGGAAGGCACG‑CATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGGTAAC‑GACCGGGCCTTGCACCGGAGCATATTCCGCGCCTGACCGAGCGACTTTATCGCGTTg > 2:216695/1‑143 (MQ=255) gAACCATACGCCGGAAGGCACG‑CAGATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGG‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATaa < 1:343126/143‑1 (MQ=255) tCATACGCCGGAAGGCACG‑CATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCGGGGATTGCAGCGGAGCGTATTCCGCGCCTGGCCGAGCGTGTTTATCGCGTTGATAAAgc > 1:5601/1‑143 (MQ=255) cGCCGGAAGGCACG‑CATATCACCGTACGCTGGCAGAGAGTGTGGGGTGGTGGGGGATCAAGAGATCACACTTAAC‑‑AACTATCTTTGCACCGGAGCATGTTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGtt > 1:931682/1‑143 (MQ=255) cATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGTGTTGATAAAGCGCGTTCCCGGCAAACcggc > 1:687923/1‑143 (MQ=255) cACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAg > 1:101709/1‑143 (MQ=255) cACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTCACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCTCGACACACCGGCGGTAg > 2:405170/1‑143 (MQ=255) cgggcggcCAGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAgg < 2:80808/141‑1 (MQ=255) gTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATc < 1:908369/143‑1 (MQ=255) ccGCACGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTGGGGTTAGCGATCGTg > 1:196063/1‑143 (MQ=255) ggCACGGTGCCGAATTCAGCGTTTA‑AGTTAACGG‑CCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTGTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGa < 2:85275/142‑1 (MQ=255) gCACGGTGCCGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGaa > 1:760329/1‑143 (MQ=255) ccGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCtg > 1:661283/1‑143 (MQ=255) ccGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGAGTAGAGTTAGCGAGCGTGAAACATGCtg > 2:44654/1‑143 (MQ=255) ccGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGGTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGTCGGTAGCGGATGAGGGTTAGCGATCGTGAAACATGCtg > 2:220771/1‑143 (MQ=255) cGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCtgt < 1:790997/143‑1 (MQ=255) cGAATTTAGCGTTGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCtgt > 1:633740/1‑143 (MQ=255) gggtGA‑AGATAAC‑GACCGGGCATTGCACCGGAGGATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAAtcatca < 1:632810/140‑1 (MQ=255) cggtGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAAtcatca < 1:725241/140‑1 (MQ=255) tGA‑AGATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAATCATCACGa > 2:502802/1‑143 (MQ=255) gATAAC‑GACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAATCATCACGAAAGt > 1:529962/1‑143 (MQ=255) | GGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACG‑CATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA‑AGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAATCATCACGAAAGT > NC_000913/418818‑419097 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |