Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,157,900 +A coding (32/1434 nt) ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,157,8961.A84.6% 90.8 / 13.4 40coding (28/1434 nt)ptsGfused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base . (5/1);  new base A (16/17);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 1.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGT  >  NC_000913/1157756‑1158032
                                                                                                                                             |                                                                                                                                          
aCTCTGGGCAATAAATAAAGGTCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCaa                                                                                                                                         <  2:748978/143‑1 (MQ=255)
    tgtgTAATAAATAAAGGGCGCTTATATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAACGCACCCATACTCACGAGCACTCTCAACTATGTTTAAGAATGCATTTGCTAACCTGCaaaaag                                                                                                                                    >  2:435556/1‑141 (MQ=255)
    tgtgTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCCATCTCAGGATCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCaaaaag                                                                                                                                    >  1:211206/1‑141 (MQ=255)
    tgtgTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCACTTATGTTTAAGAATGCATTTGCTAACCTGCaacaaa                                                                                                                                     >  1:526199/1‑139 (MQ=255)
       gtAATAATTAAACGGCGCCTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCaaaaaggtc                                                                                                                                 <  2:105559/143‑6 (MQ=255)
        taataaATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAACAAAGCACCCATACTCAGGAGCACTCTCAATCATGTTTAAGAATGCATTTGCGAACCTACAACAAGGTCg                                                                                                                                 >  1:445361/1‑143 (MQ=255)
              ataaaGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAAGGTCGGTAAAt                                                                                                                           >  1:734758/1‑143 (MQ=255)
              ataaaGGGCGCTTAGATGCCCTGTACACGGCCACGTGCGCGCCGCTTGTCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAAGGTCGGTAAAt                                                                                                                           <  2:904376/143‑1 (MQ=255)
                  aGGGCGCTAACATGCCCGGTACACGGCGAGGCTCTCCCCCCGTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCt                                                                                                                       <  2:37834/143‑1 (MQ=255)
                                  cTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGCGCACTCTCAATTATGTTTAAGAATGCCGTTGCTAACCTGCAACCAGGCCCCGCGATCGCTGATGCTGCCGGTATcc                                                                                                       >  1:830981/1‑143 (MQ=255)
                                      acacGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATGCTCAGGAGCACTCTCAATTATGTTTAAGAATGGATTTGCTAACCTGCAAGAAGGTCGGTAAATCGCGGATGCTGCCGGTATCGGtcc                                                                                                   >  1:228547/1‑141 (MQ=255)
                                         cGGCGAGGCACCCCCCCCTAGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCACAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGc                                                                                                <  2:814805/143‑1 (MQ=255)
                                          ggCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAACAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAAGGACGGTAAATCGCTGATGCTGCCGGTATCAGTACTgac                                                                                               >  2:491381/1‑141 (MQ=255)
                                                gCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGTAGCCCTCTCACTTATGTTTAAGAATGCATTTGCTAACCTCCACAACGGTCGGTGAGTCGCTGATGCTGCCGGTATCCGTACTGCCTATcac                                                                                         >  1:515495/1‑141 (MQ=255)
                                                 ctttcCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCa                                                                                        <  1:14280/140‑1 (MQ=255)
                                                  tctcCCACCTTGCCACGCGGGAGAAAGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAACTATGTTTAAGAATGCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAg                                                                                       <  2:774659/143‑1 (MQ=255)
                                                      cccccTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCGGATGCTGCCGGTATCAGTACTGCATATCGCAgacaa                                                                                   >  2:92649/1‑139 (MQ=255)
                                                        cccTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATCCTCAGGAGCCCTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCACGTAGTc                                                                                 >  1:514325/1‑143 (MQ=255)
                                                          cTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTctg                                                                               <  1:266746/143‑1 (MQ=255)
                                                           ttGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTctgc                                                                              >  2:824991/1‑143 (MQ=255)
                                                                  gcgTGAGAACGTAATAAAAGCACCCATACTCAGGAGCACTCTCAACAATGTTTAAGAATGCATTTGCTAGCCTTCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGt                                                                       <  1:254578/143‑1 (MQ=255)
                                                                     tGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTCTACGTACTGCCCATCGCAGGTATTCTACTGGGCGTCgg                                                                    >  2:879764/1‑143 (MQ=255)
                                                                      gagaACGTAAAAAAAGCACCCATACTCAGGAGCACTCAAAATTATGAATAAGAATGCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCATACTGCCTCTCGCAGGTATTCTGCCGGGCGGCGGt                                                                   >  2:509433/1‑143 (MQ=255)
                                                                           cGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCcgc                                                              <  1:616040/143‑1 (MQ=255)
                                                                                 aaaaGCACCCATACTCAGTAGCACTCACAATTATGTTTAAGAATGCAGTGGCGAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAAttt                                                        <  1:274812/143‑1 (MQ=255)
                                                                                  ttagcatttaTACTGAGTTGCACTTTCAATTATGTTTAAGAATGCAGGCGCGAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTc                                                       <  1:222172/134‑1 (MQ=255)
                                                                                  gaaGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTc                                                       <  1:726783/142‑1 (MQ=255)
                                                                                   aaGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCa                                                      <  1:337346/143‑1 (MQ=255)
                                                                                                 aggaggACGCTCAATGATGTTTAAGAATGCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCccgcc                                        <  1:235788/143‑1 (MQ=255)
                                                                                                 aGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTTCccgcc                                        >  1:729449/1‑143 (MQ=255)
                                                                                                      cACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGt                                   >  1:912015/1‑143 (MQ=255)
                                                                                                               ttATGTTTAAGAATGCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGt                          >  1:502452/1‑143 (MQ=255)
                                                                                                                   gTTTAAGAATGCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATg                      <  2:533768/143‑1 (MQ=255)
                                                                                                                   gTTTAAGAATGCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATg                      <  1:20446/143‑1 (MQ=255)
                                                                                                                    tttAAGAATGCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGATTCCGCGAATTTCAGCTGGCTGCCCGCCGgtgtctgcgctgttccgg                     >  2:315592/1‑129 (MQ=255)
                                                                                                                    gttAAGAATGCATGTGCTAACCTGCAAAAAGGGCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATgg                     <  2:921432/142‑1 (MQ=255)
                                                                                                                          aaggCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTAGCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAg               <  2:411784/140‑1 (MQ=255)
                                                                                                                          aaTGCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGATCTATCCCATCGTATGACCGAAg               >  2:293970/1‑143 (MQ=255)
                                                                                                                          aaTGCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGGAGTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCGGTTGTATCGCATGTTATGGCAGGAg               >  1:159687/1‑143 (MQ=255)
                                                                                                                             gCATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGGTATGGccgccggcg            >  1:62926/1‑135 (MQ=255)
                                                                                                                             gCATTGGCTAACCTGCGTAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCCTACTGCCTATCGCAGGGATTCTGCTGTGCGTCGGTTTCGCGAATTTCAGCTGGCTGCCCACCGGTGAATCGCTTGTTATGGCAGAAGCAg            <  2:914864/143‑1 (MQ=255)
                                                                                                                              cATTTGCTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAgg           <  1:541036/143‑1 (MQ=255)
                                                                                                                                    cTAACCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGAAGAAGCAGGCGGTTc     >  1:887244/1‑143 (MQ=255)
                                                                                                                                      aaCCTGCAAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCg   >  1:416822/1‑143 (MQ=255)
                                                                                                                                       aCCTGCACAAAGGTCGGTAAATCGCTGATGCTGCCGGCATCAGTACTGCCTGTCGCACGTATCCAGCAGGGCGACGGTTCCGCGAATGTCACCGGGCGGCCCGCCGATGCATCGCACGTTATGCCACcagcagtccgtccaat  >  2:56859/1‑133 (MQ=255)
                                                                                                                                             |                                                                                                                                          
ACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGT  >  NC_000913/1157756‑1158032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: