| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,726,390 | G→T | R123L (CGC→CTC) | nemR → | transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,726,390 | 0 | G | T | 96.4% | 153.9 / NA | 56 | R123L (CGC→CTC) | nemR | transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base T (27/27); total (27/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTT > NC_000913/1726250‑1726526 | gtccgaggAAGCTCACTGCCGCGACCGCATACCGGCTTATTACCAGCAAACTCTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCa < 2:926173/136‑1 (MQ=255) cGAAGGTAACTACCGCAACCGGATCCTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGa < 2:391362/143‑1 (MQ=255) aaGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCACACACTGAACCGCTTTTGCCAACATGGAACCATCTGTGGTTGCCTGACGTTAAAACTCTCTGCCGGAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATg < 2:627084/143‑1 (MQ=255) aGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATgg < 1:768068/143‑1 (MQ=255) gTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGAt > 1:160551/1‑143 (MQ=255) cTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGTAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAg < 1:393335/143‑1 (MQ=255) tACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAgg > 1:522700/1‑143 (MQ=255) tACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGCGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGTGCGATGGATGAAgg > 2:686958/1‑143 (MQ=255) cgcgACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGc > 1:871422/1‑143 (MQ=255) ccGCATACTGGCTTCTTACCAGCAAACTCTGACCCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTcgcg < 2:443743/143‑1 (MQ=255) aCTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGa < 1:136454/143‑1 (MQ=255) tGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGAGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGAta > 2:751501/1‑142 (MQ=255) attaCCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTg < 2:712175/143‑1 (MQ=255) cAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTGAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGctctc > 1:296513/1‑143 (MQ=255) cAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGctctc > 2:384145/1‑143 (MQ=255) aGCAAACAGTGAACCAGTTTTGCCAACAGGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTTAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGctctct < 2:200907/143‑1 (MQ=255) aGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGctctct > 1:659103/1‑143 (MQ=255) aaCCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGa > 2:534493/1‑143 (MQ=255) aCCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGGGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGaa > 1:86153/1‑143 (MQ=255) ccAGTTTTGGCAACAGGGAATCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGACATGCTGAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTAGaaa < 1:173473/143‑1 (MQ=255) cAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGaaaa > 1:759165/1‑143 (MQ=255) acttttGCCAGCATGGAACCGTGAGTGCTTGCCTGACAGTAATACTCTCTGGCGAAGTGTGCGGTCTGTAAGAAGGTATGATCGACGCGATGAATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAAt < 2:464557/141‑1 (MQ=255) aGTTTTGCCGACATGGAGCGTTGGGTGGTGGCCTGGCGGTAAACGTCGCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAAt < 2:153344/143‑1 (MQ=255) ttatGCCAACATGGACCCAGCAGTGGGTGCCTGACAGTAAAACTCTCGGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATgg < 2:573731/140‑1 (MQ=255) gCCAACAGCGAACCCTGAGTGCTTGCCTGACAGTAAAACTCCCTGCCGAAGTCTGCGATCTGTCAGAAGACACGCTCAGCGCGATGGATACAGGTGCTCGCGGCGTGACCGCCCTGCTCTCTCCGGCGCTGGAAAATGGCCGt < 2:181436/143‑1 (MQ=255) ccAACATGGAGCCATCAGTGATTGCATGACAGTAAAACTCTCTGCCGAAGTGTGCGCTCTGTCAGAGGCACTGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGAACGCCGTGCTCTCTCAGACGCTGGAAAAGGGACggg > 2:622986/1‑141 (MQ=255) ccAACATGCAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAAGCGCTGGAAAATGGCCGTg < 2:766954/143‑1 (MQ=255) aCATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTgaga > 1:251456/1‑143 (MQ=255) aTGGAACCATCCGTGGTCGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGAGCTGTCCGAAGATATGCTCAGCGCACTGGATAAAGGTGCTCGAGGCGTGGTCGCCCTGCTCTCTCAGGAGCTGGACAATGGCCGCGAGaaa > 2:171475/1‑142 (MQ=255) tGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGGAcc > 1:726880/1‑143 (MQ=255) tGGAACCATCAGTGGTTGCCTGACAGTAAAACCCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAAcc < 1:433154/143‑1 (MQ=255) ccATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGt > 2:570635/1‑143 (MQ=255) cATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGCAGATATGCTCCGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCCCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGtg > 1:99274/1‑142 (MQ=255) aTCAGTGGTTGGCTGAGAGTAAATCTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGttt < 1:23484/143‑1 (MQ=255) aGTGTTTGCCTGACAGTAAACCTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAAc < 1:905882/143‑1 (MQ=255) ttGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCtttt < 2:160551/143‑1 (MQ=255) cTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTgg < 1:409953/143‑1 (MQ=255) tGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGc > 1:1892/1‑143 (MQ=255) aaaCTGTCGGCCGGAGTGTGCGATCTGTCAGAGGATGTGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCt < 2:490176/143‑1 (MQ=255) aaCTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGAGCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACGTTTTGTGGCGAACCGCTg > 2:53712/1‑143 (MQ=255) aCTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGc < 1:137897/143‑1 (MQ=255) tctctGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCaa < 2:759165/143‑1 (MQ=255) ctctGCCGAAGTGTGCGATCTGTCAGAATATATGCTCAGCGCGATGGATAAAGGTGCGCGCGGCGGGAGCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAAc < 1:507464/143‑1 (MQ=255) tctGCCGAAGTGTGCGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACa > 1:293349/1‑143 (MQ=255) tgttggctgggTCAGGGGATAGGCGTAGCGCGATTGATATAGGTGGTCGCGGCGTGATCGCCCTGCGCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTCGTGGCGAACCGCTGCAACAGGCACACGTg < 2:816015/133‑1 (MQ=255) gCGATCCGTCAGACGATACGCTCAGCGCGATGGATAAAGGTTCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGTGAACCGCTGCAACAGGCACAAGTGCtt < 1:841959/143‑1 (MQ=255) cGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCttt < 1:750839/143‑1 (MQ=255) cGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCttt > 1:89481/1‑143 (MQ=255) cGATCTGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATAGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACACGTGCttt > 1:485198/1‑143 (MQ=255) aTCTTTCAGAATATATGCTCAGCCCCATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCCGGCGCTGGCAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTAc < 1:240103/143‑1 (MQ=255) tGTCAGAAGATATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGCCCCAAGTGCTTTAcccc > 1:791215/1‑140 (MQ=255) agaagaTATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACtgt > 1:573726/1‑143 (MQ=255) agaagaTATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCAGGAAAATGGCCGTGAGAACCATTGTTTAACTTCCTCTTTGGAACCGCTCCAACAGGCACAAGTGCTTTACGCACtgt > 2:804349/1‑143 (MQ=255) aagaTATGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTgg > 1:772444/1‑143 (MQ=255) agaTATGCTCAGCGCGTTGTATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCGCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGc < 2:217468/143‑1 (MQ=255) atatGCTCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGCGCACCTCTGTTGAACCTTATGTGTCGTACCGCTGCAACAGGCACAAGTTCTTTACGCACCGTTGcct > 2:195828/1‑141 (MQ=255) | ATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTT > NC_000913/1726250‑1726526 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |