| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 97.5% | 114.9 / NA | 40 | I204S (ATC→AGC) | wcaA | putative glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (16/23); total (17/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGG > NC_000913/2132654‑2132924 | gttgagGGTAAAGAGCGGATATTGTGTGGTGGGGCTGTCGAATGTGGCTTTGTGCTTGCGGTAAAAGTGGAAGTGCCCGGAGAATTTTGTCGGTGACGAGGTGATCTGCATTTGACCGTGATTGATATGCAGGCTCTGCGTCg < 2:614405/140‑1 (MQ=255) tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATGGATATGCAGGCTCTGCGTCGc > 1:643432/1‑143 (MQ=255) gagGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGAAGAGGAGTGCTTCGTTTCACCGTGCTTGATATGCAGGCTCTGCGTCGCct > 1:676030/1‑143 (MQ=255) ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCGCTGCGTCGCCtctt > 2:529995/1‑143 (MQ=255) gTAAACCGCTGATATTTTGTGCTGGCGCGGTCGAATTTGTCTTTGCGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttc < 1:72187/143‑1 (MQ=255) aGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGGACCCGGAGAGTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCGGGCTCTGCGCCGCCTCCactactgc > 2:43710/1‑141 (MQ=255) gATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGTTGATCTGCATTTCACCGTGATTGATATGCTGGATGTGCGGCGCGTGTTCTACTGTCCaa > 1:877197/1‑142 (MQ=255) ttttttGCTGGCGCGGTCGAATTTGTATCTCAGCATTGGCTAAAATTTGAAGTACCCGGAGAATTTTTTCCGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAATTTCCAAGGtt > 1:640353/1‑143 (MQ=255) tttgGCTGGCGCGGTCGAATTTGTCTTTGTGCGTGCGGTAAAAGTGGAAGTACCCGGAGAATGTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCg < 2:687519/143‑1 (MQ=255) cTGGCGCGGTCGAATTTGTCTCTGTGCATGCGGTAGAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa < 1:249677/143‑1 (MQ=255) tGGCTCGGTCGCCGTGGTCTTCGTGGTTGCGGTTCAAGTGGAAGTACCCGGAGAATGTTTTCGGTGACGAGGTGATCTGCCTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTGCGCCGTAc < 2:24128/143‑1 (MQ=255) tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccac > 1:483333/1‑143 (MQ=255) tCTGTGTGCGTGCGGTAAAAGTGGAAGTACCCGTAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAg < 1:408768/143‑1 (MQ=255) gtctatagCTTGCGGTAAAAGTGGAAGTACCCGGTGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAg < 1:26178/136‑1 (MQ=255) tttGTGCTTACGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTGGCCTCTTCTACTTTACACGGTTCGCCGTACTCCACCACCATCCGCAgca > 2:69901/1‑141 (MQ=255) ttGTGCTTGCGGTAAAAGGGGAGGTACCCGGAGAATGTGTGCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa < 1:338654/143‑1 (MQ=255) ttGTGCTTACTGCAAAAGTTGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTAATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa < 1:764357/143‑1 (MQ=255) tagtggagtcaGTACCCTGGGTAGTGTTTCGGTGACGAGGTGGTCTGCATTTCACCGTGACTGGTGTGCAGCCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATc < 2:10525/133‑1 (MQ=255) gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg < 1:714714/143‑1 (MQ=255) cccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa < 1:902095/143‑1 (MQ=255) ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAg > 1:584722/1‑143 (MQ=255) cgaACTTTTTCGGCGCCGAGGTGATCTGCGTCTCACCGTGATTGATACGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCACCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg < 1:691647/142‑1 (MQ=255) ttCGGTGACGAGGTGATCTGCACTTCGCCGTGATTGATATGCAGGCGCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa < 2:244595/143‑1 (MQ=255) gTGACGAGGCGATCTGCATTTCACCCTGATTGATCTGCACGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCCTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa < 1:493091/143‑1 (MQ=255) tGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa > 1:438287/1‑125 (MQ=255) tGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa < 2:438287/125‑1 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGCATCGAACAAACACTCTTTGaa > 2:337536/1‑143 (MQ=255) gCATTTCACCGTGATTGATATGCAGGCTCTGCGTAGCCTCTTCTACTTTCCACGGATCGCCATACTCCACCACCATCCGCAGGAAGATGTAGTACACCTGTGCCGCTTTCAGTTCGGGCTGGAACGAACACTGTTTgcaccct > 1:206691/1‑137 (MQ=255) tttCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTGCTCTACCACCATCCGCAGGAAGCTGTCGTAATCCTGTGCCGCTTTCCGTTCGGTATCGAAAAAACCCTCTTTGAAACGCCAt > 2:155080/1‑143 (MQ=255) cttCACTGTGCTTGTCATGCAGTCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCTGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCAt < 2:737424/142‑1 (MQ=255) tccGTGGTTGTTACGCGGGCTCTTCCTCGCCTCTTCTTCTTTCCACGTTTCGCCGTACTCCTCCACCATCCGCAGGAAGATGTCTGCTTTCTTTTCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGccc < 2:230140/142‑1 (MQ=255) ccGTTATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCa < 2:851760/143‑1 (MQ=255) ccGTCATTGATATGCAGGCTCTGCGTCGCCCCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCa < 1:403427/143‑1 (MQ=255) cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCTTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAg < 1:484424/143‑1 (MQ=255) cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAATCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAg < 1:834414/143‑1 (MQ=255) gTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAgg < 1:583783/143‑1 (MQ=255) tatGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAACCGCCATGCCCAGGTACAGAcc > 1:610685/1‑143 (MQ=255) atGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCt > 1:818520/1‑143 (MQ=255) tGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTCCTCCTGTGCCGCTTTCCGTTCGGTATCGACCACACACTCTTTGAGACGCGATGCCCCGGTAAAGAcccg > 2:663360/1‑141 (MQ=255) gCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAGAGACCTgg > 1:647083/1‑143 (MQ=255) | GTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGG > NC_000913/2132654‑2132924 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |