Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	765,191	Δ1 bp	coding (685/723 nt)	mngR ←	DNA‑binding transcriptional repressor MngR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	765,189	0	T	.	100.0%	396.8 / NA	83	coding (687/723 nt)	mngR	DNA‑binding transcriptional repressor MngR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (42/41);  total (42/41)

ATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGG  >  NC_000913/765155‑765219                                   |                               aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                >  1:3399670/1‑35 (MQ=255) aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                <  1:3718906/35‑1 (MQ=255) aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                >  1:263304/1‑35 (MQ=255) aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                >  1:4030158/1‑35 (MQ=255) aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                >  1:4823703/1‑35 (MQ=255) aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                <  1:2145379/35‑1 (MQ=255) aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                >  1:5236152/1‑35 (MQ=255)  tCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtt                               <  1:3094226/35‑1 (MQ=255)  tCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtt                               >  1:2327953/1‑35 (MQ=255)  tCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtt                               <  1:7370299/35‑1 (MQ=255)  tCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtt                               >  1:7096523/1‑35 (MQ=255)  tCGCGATGATTTTCGCTGCGCTATCAGGGGAAAtt                               >  1:2341783/1‑35 (MQ=255)   cgcgATGATTTTCGCTGCGCTATCAGGGTAAAtta                              <  1:7175360/35‑2 (MQ=255)   cgcgATGATTTTCGCTGCGCTATCAGGGTAAAtta                              <  1:570014/35‑2 (MQ=255)       atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                         >  1:4952108/1‑35 (MQ=37)       atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                         <  1:2671837/35‑1 (MQ=37)        tgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTc                        >  1:4142554/1‑35 (MQ=37)        tgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTc                        >  1:2832227/1‑35 (MQ=37)        tgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTc                        >  1:2612146/1‑35 (MQ=37)        tgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTc                        <  1:7613771/35‑1 (MQ=37)        tgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTc                        >  1:1334451/1‑35 (MQ=37)          atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                      >  1:3099842/1‑35 (MQ=37)          atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                      >  1:3658418/1‑35 (MQ=37)          atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                      >  1:6954615/1‑35 (MQ=37)          atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                      >  1:6217840/1‑35 (MQ=37)          atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                      >  1:61935/1‑35 (MQ=37)          atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                      >  1:49474/1‑35 (MQ=37)          atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                      <  1:6832679/35‑1 (MQ=37)           ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                     <  1:6494774/35‑1 (MQ=37)           ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                     >  1:3120506/1‑35 (MQ=37)           ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                     >  1:1090760/1‑35 (MQ=37)           ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                     >  1:7619212/1‑35 (MQ=37)           ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                     >  1:3754551/1‑35 (MQ=37)           ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                     <  1:6494718/35‑1 (MQ=37)           ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                     >  1:3754611/1‑35 (MQ=37)           ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                     >  1:6896955/1‑35 (MQ=37)             ttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                   <  1:637900/35‑1 (MQ=38)             ttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                   >  1:3100862/1‑35 (MQ=38)             ttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                   >  1:2029058/1‑35 (MQ=38)             ttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                   <  1:2741522/35‑1 (MQ=38)             ttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                   <  1:6651419/35‑1 (MQ=38)              tCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGt                  >  1:5698600/1‑35 (MQ=38)              tCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGt                  >  1:5569989/1‑35 (MQ=38)               cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTa                 <  1:3245375/35‑1 (MQ=38)               cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTa                 <  1:1540959/35‑1 (MQ=38)                gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                <  1:5149892/35‑1 (MQ=38)                gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                >  1:1319548/1‑35 (MQ=38)                gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                >  1:4441277/1‑35 (MQ=38)                gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                <  1:4291665/35‑1 (MQ=38)                gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                <  1:2628104/35‑1 (MQ=38)                gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                <  1:6368921/35‑1 (MQ=38)                gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                >  1:6115437/1‑35 (MQ=38)                 cTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAtt               >  1:956624/1‑35 (MQ=38)                 cTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAtt               <  1:7177811/35‑1 (MQ=38)                 cTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAtt               <  1:6166180/35‑1 (MQ=38)                 cTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAtt               <  1:3378782/35‑1 (MQ=38)                  tGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA              <  1:1160616‑M1/35‑1 (MQ=255)                  tGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA              >  1:120883‑M1/1‑35 (MQ=255)                  tGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA              <  1:446875‑M1/35‑1 (MQ=255)                  tGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA              <  1:3589633‑M1/35‑1 (MQ=255)                  tGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA              <  1:6877955‑M1/35‑1 (MQ=255)                  tGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA              <  1:1702358‑M1/35‑1 (MQ=255)                   gcgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAt             <  1:7270790‑M1/35‑2 (MQ=255)                   gcgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAt             <  1:6321924‑M1/35‑2 (MQ=255)                    cgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAta            <  1:7443494‑M1/35‑3 (MQ=255)                    cgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAta            <  1:3241812‑M1/35‑3 (MQ=255)                     gcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag           <  1:3717129‑M1/35‑4 (MQ=255)                     gcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag           <  1:3473930‑M1/35‑4 (MQ=255)                      cTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagc          >  1:336580‑M1/1‑31 (MQ=255)                      cTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagc          >  1:6225796‑M1/1‑31 (MQ=255)                       tATCAGGGTAAA‑TTATAGTCATCGGTATTAtagca         >  1:7612024‑M1/1‑30 (MQ=255)                        aTCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaa        >  1:2746037‑M1/1‑29 (MQ=255)                        aTCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaa        >  1:5754177‑M1/1‑29 (MQ=255)                         tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat       <  1:7436182‑M1/35‑8 (MQ=255)                         tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat       >  1:2778203‑M1/1‑28 (MQ=255)                         tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat       >  1:6620904‑M1/1‑28 (MQ=255)                         tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat       >  1:1713200‑M1/1‑28 (MQ=255)                         tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat       <  1:1766852‑M1/35‑8 (MQ=255)                         tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat       >  1:1973153‑M1/1‑28 (MQ=255)                         tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat       >  1:6921941‑M1/1‑28 (MQ=255)                          cAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatc      <  1:6648588‑M1/35‑9 (MQ=255)                          cAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatc      >  1:2035280‑M1/1‑27 (MQ=255)                          cAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatc      <  1:2193595‑M1/35‑9 (MQ=255)                           aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatca     <  1:2033380‑M1/35‑10 (MQ=255)                           aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatca     >  1:7343822‑M1/1‑26 (MQ=255)                           aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatca     >  1:144720‑M1/1‑26 (MQ=255)                            gggTAAA‑TTATAGTCATCGGTATTAtagcaatcaa    <  1:6077679‑M1/35‑11 (MQ=255)                            gggTAAA‑TTATAGTCATCGGTATTAtagcaatcaa    >  1:5329927‑M1/1‑25 (MQ=255)                            gggTAAA‑TTATAGTCATCGGTATTAtagcaatcaa    <  1:4600787‑M1/35‑11 (MQ=255)                             ggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa   <  1:5131863‑M1/35‑12 (MQ=255)                             ggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa   >  1:3064625‑M1/1‑24 (MQ=255)                             ggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa   <  1:7361668‑M1/35‑12 (MQ=255)                             ggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa   >  1:3375584‑M1/1‑24 (MQ=255)                             ggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa   <  1:6152103‑M1/35‑12 (MQ=255)                              gTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac  <  1:1985304‑M1/35‑13 (MQ=255)                              gTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac  <  1:585308‑M1/35‑13 (MQ=255)                              gTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac  <  1:864734‑M1/35‑13 (MQ=255)                                   |                               ATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGG  >  NC_000913/765155‑765219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑