Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,720,597 Δ1 bp coding (4/153 nt) hokA ← small toxic polypeptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,720,5960G.100.0% 442.1 / NA 102coding (5/153 nt)hokAsmall toxic polypeptide
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (72/30);  total (72/30)

Only 100 of 109 total aligned reads displayed.

AATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAA  >  NC_000913/3720562‑3720626
                                  |                              
aaTCACTATTAAAGAAAGTAATCTATATTTCTGCg                                >  1:5125233/1‑35 (MQ=255)
aaTCACTATTAAAGAAAGTAATCTATATTTCTGCg                                >  1:6556901/1‑35 (MQ=255)
aaTCACTATTAAAGAAAGTAATCTATATTTCTGCg                                <  1:7422498/35‑1 (MQ=255)
 aTCACTATTAAAGAAAGTAATCTATATTTCTGCgc                               >  1:5134551/1‑34 (MQ=255)
 aTCACTATTAAAGAAAGTAATCTATATTTCTGCgc                               >  1:1399935/1‑34 (MQ=255)
 aTCACTATTAAAGAAAGTAATCTATATTTCTGCgc                               >  1:613213/1‑34 (MQ=255)
     cTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTc                          >  1:6434127/1‑35 (MQ=38)
     cTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTc                          <  1:3274354/35‑1 (MQ=38)
     cTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTc                          >  1:7481188/1‑35 (MQ=38)
      tATTAAAGAAAGTAATCTATATTTCTGC‑GCATTcc                         >  1:7581496/1‑35 (MQ=38)
      tATTAAAGAAAGTAATCTATATTTCTGC‑GCATTcc                         <  1:5753967/35‑1 (MQ=38)
       aTTAAAGAAAGTAATCTATATTTCTGC‑GCATTccc                        >  1:5032927/1‑35 (MQ=38)
       aTTAAAGAAAGTAATCTATATTTCTGC‑GCATTccc                        <  1:2060307/35‑1 (MQ=38)
       aTTAAAGAAAGTAATCTATATTTCTGC‑GCATTccc                        >  1:1343920/1‑35 (MQ=38)
        ttAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCa                       >  1:579609/1‑35 (MQ=38)
        ttAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCa                       >  1:264389/1‑35 (MQ=38)
        ttAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCa                       >  1:5013827/1‑35 (MQ=38)
        ttAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCa                       >  1:2701618/1‑35 (MQ=38)
        ttAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCa                       >  1:5537043/1‑35 (MQ=38)
        ttAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCa                       >  1:632303/1‑35 (MQ=38)
        ttAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCa                       >  1:4646821/1‑35 (MQ=38)
        ttAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCa                       >  1:1869436/1‑35 (MQ=38)
        ttAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCa                       >  1:4593294/1‑35 (MQ=38)
        ttAAACAAAGTAATCTATATTTCTGC‑GCATTCCCa                       >  1:7843443/1‑35 (MQ=25)
         tAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAg                      >  1:4267236/1‑35 (MQ=38)
          aaagaaagTAATCTATATTTCTGC‑GCATTCCCAGc                     >  1:4088092/1‑35 (MQ=38)
          aaagaaagTAATCTATATTTCTGC‑GCATTCCCAGc                     >  1:5933303/1‑35 (MQ=38)
          aaagaaagTAATCTATATTTCTGC‑GCATTCCCAGc                     >  1:7728309/1‑35 (MQ=38)
          aaagaaagTAATCTATATTTCTGC‑GCATTCCCAGc                     >  1:230907/1‑35 (MQ=38)
          aaagaaagTAATCTATATTTCTGC‑GCATTCCCAGc                     >  1:2129889/1‑35 (MQ=38)
           aagaaagTAATCTATATTTCTGC‑GCATTCCCAGcc                    <  1:3943617/35‑1 (MQ=38)
           aagaaagTAATCTATATTTCTGC‑GCATTCCCAGcc                    >  1:2474565/1‑35 (MQ=38)
           aagaaagTAATCTATATTTCTGC‑GCATTCCCAGcc                    >  1:1779981/1‑35 (MQ=38)
            agaaagTAATCTATATTTCTGC‑GCATTCCCAGcct                   <  1:2630856/35‑1 (MQ=38)
             gaaagTAATCTATATTTCTGC‑GCATTCCCAGcctc                  <  1:5482059/35‑1 (MQ=38)
             gaaagTAATCTATATTTCTGC‑GCATTCCCAGcctc                  >  1:6494822/1‑35 (MQ=38)
              aaagTAATCTATATTTCTGC‑GCATTCCCAGcctcc                 >  1:832647/1‑35 (MQ=38)
               aagTAATCTATATTTCTGC‑GCATTCCCAGcctcct                >  1:5924533/1‑35 (MQ=38)
               aagTAATCTATATTTCTGC‑GCATTCCCAGcctcct                >  1:1119646/1‑35 (MQ=38)
               aagTAATCTATATTTCTGC‑GCATTCCCAGcctcct                >  1:7490240/1‑35 (MQ=38)
               aagTAATCTATATTTCTGC‑GCATTCCCAGcctcct                >  1:1132097/1‑35 (MQ=38)
               aagTAATCTATATTTCTGC‑GCATTCCCAGcctcct                >  1:2314332/1‑35 (MQ=38)
                agTAATCTATATTTCTGC‑GCATTCCCAGCCTCCtg               >  1:1839061/1‑35 (MQ=38)
                agTAATCTATATTTCTGC‑GCATTCCCAGCCTCCtg               >  1:7630516/1‑35 (MQ=38)
                agTAATCTATATTTCTGC‑GCATTCCCAGCCTCCtg               >  1:2654411/1‑35 (MQ=38)
                 gTAATCTATATTTCTGC‑GCATTCCCAGCCTCCtgt              >  1:7171847/1‑35 (MQ=38)
                 gTAATCTATATTTCTGC‑GCATTCCCAGCCTCCtgt              >  1:6055468/1‑35 (MQ=38)
                 gTAATCTATATTTCTGC‑GCATTCCCAGCCTCCtgt              >  1:3970978/1‑35 (MQ=38)
                 gTAATCTATATTTCTGC‑GCATTCCCAGCCTCCtgt              >  1:6084140/1‑35 (MQ=38)
                  tAATCTATATTTCTGC‑GCATTCCCAGCCTCCtgtg             >  1:4326633/1‑35 (MQ=38)
                  tAATCTATATTTCTGC‑GCATTCCCAGCCTCCtgtg             >  1:5863951/1‑35 (MQ=38)
                  tAATCTATATTTCTGC‑GCATTCCCAGCCTCCtgtg             <  1:1754084/35‑1 (MQ=38)
                  tAATCTATATTTCTGC‑GCATTCCCAGCCTCCtgtg             <  1:6739684/35‑1 (MQ=38)
                    aTCTATATTTCTGC‑GCATTGCCAGCCTCCTGTGtt           <  1:6695488/35‑1 (MQ=25)
                    aTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGtt           <  1:664993/35‑1 (MQ=38)
                    aTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGtt           >  1:2187763/1‑35 (MQ=38)
                    aTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGtt           >  1:4794728/1‑35 (MQ=38)
                    aTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGtt           <  1:7360929/35‑1 (MQ=38)
                    aTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGtt           >  1:5936337/1‑35 (MQ=38)
                    aTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGtt           >  1:5610507/1‑35 (MQ=38)
                    aTCTATATTTCGGC‑GCATTCCCAGCCTCCTGTGtt           <  1:7503331/35‑1 (MQ=25)
                     tCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTg          <  1:1855217/35‑1 (MQ=38)
                     tCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTg          >  1:5544798/1‑35 (MQ=38)
                     tCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTg          >  1:4508720/1‑35 (MQ=38)
                      cTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGa         >  1:3201650/1‑35 (MQ=38)
                      cTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGa         >  1:2284432/1‑35 (MQ=38)
                       tataTTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAt        >  1:6666322/1‑35 (MQ=38)
                       tataTTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAt        >  1:2163776/1‑35 (MQ=38)
                       tataTTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAt        >  1:4364314/1‑35 (MQ=38)
                       tataTTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAt        >  1:2483940/1‑35 (MQ=38)
                       tataTTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAt        >  1:2714611/1‑35 (MQ=38)
                       tataTTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAt        >  1:4870228/1‑35 (MQ=38)
                       tataTTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAt        >  1:426383/1‑35 (MQ=38)
                        atatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAtt       >  1:5387895/1‑35 (MQ=38)
                        atatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAtt       <  1:1753624/35‑1 (MQ=38)
                        atatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAtt       >  1:4945866/1‑35 (MQ=38)
                        atatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAtt       >  1:7683704/1‑35 (MQ=38)
                         tatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAttt      <  1:7298138/35‑1 (MQ=38)
                         tatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAttt      >  1:1019986/1‑35 (MQ=38)
                         tatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAttt      >  1:5188195/1‑35 (MQ=38)
                          atTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTc     <  1:4861822/35‑1 (MQ=38)
                          atTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTc     <  1:5844189/35‑1 (MQ=38)
                          atTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTc     <  1:6939732/35‑1 (MQ=38)
                          atTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTc     <  1:2750123/35‑1 (MQ=38)
                          atTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTc     <  1:3515271/35‑1 (MQ=38)
                           tttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTcc    <  1:6403932/35‑1 (MQ=38)
                           tttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTcc    <  1:4984337/35‑1 (MQ=38)
                           tttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTcc    >  1:1564354/1‑35 (MQ=38)
                           tttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTcc    <  1:1547925/35‑1 (MQ=38)
                           tttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTcc    >  1:4502494/1‑35 (MQ=38)
                           tttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTcc    >  1:5658284/1‑35 (MQ=38)
                           tttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTcc    >  1:6717548/1‑35 (MQ=38)
                           tttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTcc    >  1:2482011/1‑35 (MQ=38)
                            ttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCa   >  1:3687078/1‑35 (MQ=38)
                            ttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCa   >  1:605116/1‑35 (MQ=38)
                             tCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCaa  >  1:4474529/1‑35 (MQ=38)
                             tCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCaa  <  1:7678663/35‑1 (MQ=38)
                             tCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCaa  <  1:7581170/35‑1 (MQ=38)
                             tCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCaa  >  1:3158917/1‑35 (MQ=38)
                             tCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCaa  >  1:2826013/1‑35 (MQ=38)
                                  |                              
AATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAA  >  NC_000913/3720562‑3720626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑