| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,414,906 | 0 | T | G | 21.6% | 115.2 / 10.1 | 37 | V211G (GTG→GGG) | aidB | putative acyl‑CoA dehydrogenase AidB |
| Reads supporting (aligned to +/- strand): ref base T (4/25); new base G (8/0); total (12/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.28e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGC > NC_000913/4414872‑4414940 | gCAGAGCGTCTGGAAGATGGCTCTTATCGGCtggt < 1:1615581/35‑1 (MQ=255) agagCGTCTGGAAGATGGCTCTTATCGGCtggtgg < 1:1637282/35‑1 (MQ=255) gagCGTCTGGAAGATGGCTCTTATCGGCTgggggg > 1:3729400/1‑35 (MQ=255) agCGTCTGGAAGATGGCTCTTATCGGCTGGTgggg < 1:4092850/35‑1 (MQ=255) cGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCa < 1:1913603/35‑1 (MQ=255) gTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCAt < 1:4380481/35‑1 (MQ=255) gTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCAt < 1:3800796/35‑1 (MQ=255) gTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCAt < 1:2515560/35‑1 (MQ=255) tCTGGAAGATGGCTCTTATCGGCTGGGGGGGGATa > 1:5773740/1‑35 (MQ=37) tCTGGAAGATGGCTCTTATCGGCTGGGGGGGGATa > 1:5331306/1‑35 (MQ=37) cTGGAAGATGGCTCTTATCGGCTGGTGGGGCATaa < 1:7147317/35‑1 (MQ=255) cTGGAAGATGGCTCTTATCGGCTGGTGGGGCATaa < 1:5480772/35‑1 (MQ=255) cTGGAAGATGGCTCTTATCGGCTGGTGGGGCATaa < 1:469858/35‑1 (MQ=255) tGGAAGATGGCTCTTATCGGCTGGTGGGGCATaaa < 1:6988010/35‑1 (MQ=255) tGGAAGATGGCTCTTATCGGCTGGTGGGGCATaaa < 1:448059/35‑1 (MQ=255) ggAAGATGGCTCTTATCGGCTGGGGGGGGATaaaa > 1:255957/1‑34 (MQ=25) aGATGGCTCTTATCGGCTGGGGGGGGATAAATggg > 1:7230377/1‑34 (MQ=25) aGATGGCTCTTATCGGCTGGGGGGGCATAAATggg > 1:4052176/1‑34 (MQ=38) aTGGCTCTTATCGGCTGGTGGGGCATAAATGGttt < 1:5499113/35‑1 (MQ=255) aTGGCTCTTATCGGCTGGTGGGGCATAAATGGttt < 1:6113851/35‑1 (MQ=255) aTGGCTCTTATCGGCTGGTGGGGCATAAATGGttt < 1:4462553/35‑1 (MQ=255) aTGGCTCTTATCGGCTGGGGGGGGGTAAATGGgtt > 1:694069/1‑35 (MQ=16) tGGCTCTTATCGGCTGGTGGGGCATAAATGGtttt < 1:7176079/35‑1 (MQ=255) tGGCTCTTATCGGCTGGTGGGGCATAAATGGtttt < 1:6025452/35‑1 (MQ=255) tGGCTCTTATCGGCTGGGGGGGGATAAATGGgttt > 1:4190148/1‑35 (MQ=25) ggCTCTTATCGGCTGGTGGGGCATAAATGGttttt < 1:1191327/35‑1 (MQ=255) gCTCTTATCGGCTGGTGGGGCATAAATGGTTTTtc < 1:6253102/35‑1 (MQ=255) gCTCTTATCGGCTGGGGGGGCATAAATGGGTTTtc > 1:2113918/1‑35 (MQ=37) ctctTATCGGCTGGTGGGGCATAAATGGTTTTtct < 1:7801235/35‑1 (MQ=255) tctTATCGGCTGGTGGGGCATAAATGGTTTTtctc < 1:5624899/35‑1 (MQ=255) ttATCGGCTGGTGGGGCATAAATGGTTTTTCTCgg > 1:935638/1‑35 (MQ=255) tATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGt < 1:5719931/35‑1 (MQ=255) tCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTc < 1:1403475/35‑1 (MQ=255) tCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTc < 1:3239805/35‑1 (MQ=255) ggCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCg < 1:7479183/35‑1 (MQ=255) gCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGc < 1:7051373/35‑1 (MQ=255) gCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGc < 1:3908436/35‑1 (MQ=255) gCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGc > 1:2000711/1‑35 (MQ=255) cTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCa > 1:6082152/1‑35 (MQ=255) cTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCa > 1:1786840/1‑35 (MQ=255) ggtggGGCATAAATGGTTTTTCTCGGTTCCGCaaa > 1:5130864/1‑35 (MQ=255) ggtggGGCATAAATGGTTTTTCTCGGTTCCGCaaa > 1:5067/1‑35 (MQ=255) ggtggGGCATAAATGGTTTTTCTCGGTTCCGCaaa < 1:4970331/35‑1 (MQ=255) ggtggGGCATAAATGGTTTTTCTCGGTTCCGCaaa > 1:4741020/1‑35 (MQ=255) gtggGGCATAAATGGTTTTTCTCGGTTCCGCAAAg > 1:4440602/1‑35 (MQ=255) tggGGCATAAATGGTTTTTCTCGGTTCCGCAAAGc < 1:718996/35‑1 (MQ=255) tggGGCATAAATGGTTTTTCTCGGTTCCGCAAAGc > 1:3213224/1‑35 (MQ=255) | GCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGC > NC_000913/4414872‑4414940 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |