| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 987,014 | 0 | A | C | 20.6% | 332.3 / 11.2 | 97 | intergenic (‑32/+571) | ompF/asnS | outer membrane porin F/asparagine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base A (60/17); new base C (0/20); total (60/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.84e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 111 total aligned reads displayed.
CATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATA > NC_000913/986980‑987046 | catTATTTATTACCCTCATGGTTTTTTTTATGaca < 1:3413120/35‑1 (MQ=255)catTATTTATTACCCTCATGGTTTTTTTTATGaca < 1:1213283/35‑1 (MQ=255) attattTATTACCCTCATGGTTTTTTTTATGacac > 1:800324/1‑35 (MQ=255) attattTATTACCCTCATGGTTTTTTTTATGacac < 1:272791/35‑1 (MQ=255) attattTATTACCCTCATGGTTTTTTTTATGacac > 1:7861240/1‑35 (MQ=255) ttatttatTACCCTCATGGTTTTTTTTATGACAcc < 1:1470759/35‑1 (MQ=255) ttatttatTACCCTCATGGTTTTTTTTATGACAcc < 1:4338455/35‑1 (MQ=255) tatttattACCCTCATGGTTTTTTTTATGACACCt < 1:7663194/35‑1 (MQ=255) tatttattACCCTCATGGTTTTTTTTATGACACCt < 1:6562171/35‑1 (MQ=255) tatttattACCCTCATGGTTTTTTTTATGACACCt < 1:4311284/35‑1 (MQ=255) atttattACCCTCATGGTTTTTTTTATGACACCTg < 1:4855551/35‑1 (MQ=255) tttattACCCTCATGGTTTTTTTTATGACACCTGc < 1:6898715/35‑1 (MQ=255) tttattACCCTCATGGTTTTTTTTATGACACCTGc > 1:6031187/1‑35 (MQ=255) tttattACCCTCATGGTTTTTTTTATGACACCTGc > 1:4836192/1‑35 (MQ=255) tttattACCCTCATGGTTTTTTTTATGACACCTGc > 1:290448/1‑35 (MQ=255) tttattACCCTCATGGTTTTTTTTATGACACCTGc > 1:1702600/1‑35 (MQ=255) tttattACCCTCATGGTTTTTTTTATGACACCTGc > 1:3711293/1‑35 (MQ=255) tttattACCCTCATGGTTTTTTTTATGACACCTGc < 1:7438115/35‑1 (MQ=255) tttattACCCTCATGGTTTTTTTTATGACACCTGc < 1:7742391/35‑1 (MQ=255) tttattACCCTCATGGTTTTTTTTATGACACCTGc > 1:7860279/1‑35 (MQ=255) ttattaCCCTCATGGTTTTTTTTATGACACCTGcc < 1:1735356/35‑1 (MQ=255) ttattaCCCTCATGGTTTTTTTTATGACACCTGcc < 1:4144631/35‑1 (MQ=255) ttattaCCCTCATGGTTTTTTTTATGACACCTGcc < 1:1995190/35‑1 (MQ=255) ttattaCCCTCATGGTTTTTTTTATGACACCTGcc < 1:6775220/35‑1 (MQ=255) ttattaCCCTCATGGTTTTTTTTATGACACCTGcc < 1:3950515/35‑1 (MQ=255) tattcCCCTCATGGTTTTTTTTATGACACCTGCCa < 1:6220087/35‑1 (MQ=255) tattcCCCTCATGGTTTTTTTTATGACACCTGCCa < 1:1424707/35‑1 (MQ=255) tattaCCCTCATGGTTTTTTTTATGACCCCTGCCa < 1:5122009/35‑1 (MQ=255) tattaCCCTCATGGTTTTTTTTATGACACCTGCCa > 1:7386146/1‑35 (MQ=255) attaCCCTCATGGTTTTTTTTATGACACCTGCCAc > 1:4433020/1‑35 (MQ=255) attaCCCTCATGGTTTTTTTTATGACACCTGCCAc > 1:6843758/1‑35 (MQ=255) attaCCCTCATGGTTTTTTTTATGACACCTGCCAc > 1:7337315/1‑35 (MQ=255) attaCCCTCATGGTTTTTTTTATGACACCTGCCAc > 1:339410/1‑35 (MQ=255) ttaCCCTCATGGTTTTTTTTATGACACCTGCCACt > 1:1230588/1‑35 (MQ=255) ttaCCCTCATGGTTTTTTTTATGACACCTGCCACt > 1:7079523/1‑35 (MQ=255) ccccTCATGTTTTTTTTTATGACACCTGCCACTGc < 1:6711255/34‑1 (MQ=38) aCCCTCATGGTTTTTTTTATGACACCTGCCACTGc > 1:5831899/1‑35 (MQ=255) aCCCTCATGGTTTTTTTTATGACACCTGCCACTGc > 1:2464193/1‑35 (MQ=255) aCCCTCATGGTTTTTTTTATGACACCTGCCACTGc > 1:3396459/1‑35 (MQ=255) cccTCATGGTTTTTTTTATGACCCCTGCCACTGcc < 1:3283618/35‑1 (MQ=255) cccTCATGGTTTTTTTTATGACACCTGCCACTGcc > 1:6851702/1‑35 (MQ=255) cccTCATGGTTTTTTTTATGACACCTGCCACTGcc > 1:916109/1‑35 (MQ=255) cccTCATGGTTTTTTTTATGACACCTGCCACTGcc > 1:2105598/1‑35 (MQ=255) cccTCATGGTTTTTTTTATGACACCTGCCACTGcc > 1:1367786/1‑35 (MQ=255) cccTCATGGTTTTTTTTATGACACCTGCCACTGcc > 1:1248360/1‑35 (MQ=255) cTCATGGTTTTTTTTATGACCCCTGCCACTGCCGt < 1:1763903/35‑1 (MQ=255) cTCATGGTTTTTTTTATGACACCTGCCACTGCCGt > 1:1044882/1‑35 (MQ=255) tCATGTTTTTTTTTATGACCCCTGCCACTGCCGTc < 1:4797209/35‑1 (MQ=38) tCATGGTTTTTTTTATGACCCCTGCCACTGCCGTc < 1:2491472/35‑1 (MQ=255) tCATGGTTTTTTTTATGACCCCTGCCACTGCCGTc < 1:1423703/35‑1 (MQ=255) tCATGGTTTTTTTTATGACACCTGCCACTGCCGTc > 1:7166897/1‑35 (MQ=255) tCATGGTTTTTTTTATGACACCTGCCACTGCCGTc > 1:2283831/1‑35 (MQ=255) aTGGTTTTTTTTATGACCCCTGCCACTGCCGTCaa < 1:3179997/35‑1 (MQ=255) aTGGTTTTTTTTATGACCCCTGCCACTGCCGTCaa < 1:2769457/35‑1 (MQ=255) aTGGTTTTTTTTATGACACCTGCCACTGCCGTCaa > 1:4908037/1‑35 (MQ=255) aTGGTTTTTTTTATGACACCTGCCACTGCCGTCaa > 1:2172819/1‑35 (MQ=255) aTGGTTTTTTTTATGACACCTGCCACTGCCGTCaa > 1:2172861/1‑35 (MQ=255) aTGGTTTTTTTTATGACACCTGCCACTGCCGTCaa > 1:7816568/1‑35 (MQ=255) tGGTTTTTTTTATGACCCCTGCCACTGCCGTCAAt < 1:1655435/35‑1 (MQ=255) tGGTTTTTTTTATGACCCCTGCCACTGCCGTCAAt < 1:6802440/35‑1 (MQ=255) tttttttttATGACCCCTGCCACTGCCGTCAATaa < 1:6427633/34‑1 (MQ=39) gTTTTTTTTATGACACCTGCCACTGCCGTCAATaa > 1:4671360/1‑35 (MQ=255) gTTTTTTTTATGACACCTGCCACTGCCGTCAATaa > 1:1792519/1‑35 (MQ=255) gTTTTTTTTACGCCCCCTGCCACTGCCGTCAATaa < 1:4074217/35‑1 (MQ=255) ttttttttATGACACCTGCCACTGCCGTCAATAAg > 1:1673600/1‑35 (MQ=255) tttttttATGCCCCCTGCCACTGCCGTCAATAAGt < 1:3229961/35‑1 (MQ=38) tttttttATGACCCCTGCCACTGCCGTCAATAAGt < 1:7129471/35‑1 (MQ=255) tttttttATGACACCTGCCACTGCCGTCAATAAGt > 1:4714777/1‑35 (MQ=255) ttttttATGCCCCCTGCCACTGCCGTCAATAAGtt < 1:6077142/35‑1 (MQ=38) ttttttATGACACCTGCCACTGCCGTCAATAAGtt > 1:1180322/1‑35 (MQ=255) ttttttATGACACCTGCCACTGCCGTCAATAAGtt > 1:4563898/1‑35 (MQ=255) ttttttATGACACCTGCCACTGCCGTCAATAAGtt > 1:2822582/1‑35 (MQ=255) tttttATGACACCTGCCACTGCCGTCAATAAGTTc > 1:1356792/1‑35 (MQ=255) tttttATGACACCTGCCACTGCCGTCAATAAGTTc > 1:6763773/1‑35 (MQ=255) tttttATGACACCTGCCACTGCCGTCAATAAGTTc > 1:6763728/1‑35 (MQ=255) ttttATGACACCTGCCACTGCCGTCAATAAGTTCt > 1:856689/1‑35 (MQ=255) tttATGCCCCCTGCCACTGCCGTCAATAAGTTCTg < 1:3605876/35‑1 (MQ=38) tttATGACCCCTGCCACTGCCGTCAATAAGTTCTg < 1:3605917/35‑1 (MQ=255) tttATGACCCCTGCCACTGCCGTCAATAAGTTCTg < 1:2401494/35‑1 (MQ=255) tttATGACACCTGCCACTGCCGTCAATAAGTTCTg > 1:7405940/1‑35 (MQ=255) ttATGACCCCTGCCACTGCCGTCAATAAGTTCTGt < 1:7069127/35‑1 (MQ=255) ttATGACACCTGCCACTGCCGTCAATAAGTTCTGt > 1:4150411/1‑35 (MQ=255) ttATGACACCTGCCACTGCCGTCAATAAGTTCTGt > 1:1716876/1‑35 (MQ=255) tATGACCCCTGCCACTGCCGTCAATAAGTTCTGTc < 1:1094371/35‑1 (MQ=255) tATGACACCTGCCACTGCCGTCAATAAGTTCTGTc > 1:3146536/1‑35 (MQ=255) tATGACACCTGCCACTGCCGTCAATAAGTTCTGTc > 1:2284331/1‑35 (MQ=255) tATGACACCTGCCACTGCCGTCAATAAGTTCTGTc > 1:2128523/1‑35 (MQ=255) aTGACCCCTGCCACTGCCGTCAATAAGTTCTGTCa < 1:1713988/35‑1 (MQ=255) tGACCCCTGCCACTGCCGTCAATAAGTTCTGTCaa < 1:5547377/35‑1 (MQ=255) tGACCCCTGCCACTGCCGTCAATAAGTTCTGTCaa < 1:6993880/35‑1 (MQ=255) tGACCCCTGCCACTGCCGTCAATAAGTGCTGTCaa < 1:8221/35‑1 (MQ=38) tGACACCTGCCACTGCCGTCAATAAGTTCTGTCaa > 1:6355808/1‑35 (MQ=255) tGACACCTGCCACTGCCGTCAATAAGTTCTGTCaa > 1:4944590/1‑35 (MQ=255) tGACACCTGCCACTGCCGTCAATAAGTTCTGTCaa > 1:7071648/1‑35 (MQ=255) tGACACCTGCCACTGCCGTCAATAAGTTCTGTCaa > 1:2019479/1‑35 (MQ=255) tGACACCTGCCACTGCCGTCAATAAGTTCTGTCaa > 1:2006973/1‑35 (MQ=255) gACACCTGCCACTGCCGTCAATAAGTTCTGTCAAt > 1:5063446/1‑35 (MQ=255) acacCTGCCACTGCCGTCAATAAGTTCTGTCAATa > 1:6274214/1‑35 (MQ=255) acacCTGCCACTGCCGTCAATAAGTTCTGTCAATa > 1:6483407/1‑35 (MQ=255) acacCTGCCACTGCCGTCAATAAGTTCTGTCAATa > 1:5224538/1‑35 (MQ=255) | CATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATA > NC_000913/986980‑987046 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |