Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	988,499	G→A	P163S (CCA→TCA)	asnS ←	asparagine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	988,499	0	G	A	100.0%	292.4 / NA	71	P163S (CCA→TCA)	asnS	asparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/39);  total (32/39)

TGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTA  >  NC_000913/988467‑988530                                 |                                tGCACCTTCGGTATCAGATGCGGTAATCAGTGACg                               <  1:3799457/35‑1 (MQ=255) tGCACCTTCGGTATCAGATGCGGTAATCAGTGACg                               <  1:1640977/35‑1 (MQ=255)  gCACCTTCGGTATCAGATGCGGTAATCAGTGACGt                              <  1:6865720/35‑1 (MQ=38)  gCACCTTCGGTATCAGATGCGGTAATCAGTGACGt                              <  1:2417031/35‑1 (MQ=255)  gCACCTTCGGTATCAGATGCGGTAATCAGTGACGt                              <  1:7272490/35‑1 (MQ=38)   cACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                             <  1:3130944/35‑1 (MQ=38)   cACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                             >  1:2828477/1‑35 (MQ=38)    aCCTTCGGTATCAGATGCGGTAATCAGTGACGTTg                            <  1:5165731/35‑1 (MQ=38)     ccTTCGGTATCAGATGCGGTAATCAGTGACGTTGa                           <  1:7335085/35‑1 (MQ=38)         cGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                       <  1:2734900/35‑1 (MQ=38)          ggTATCAGATGCGGTAATCAGTGACGTTGAAAccc                      <  1:7881955/35‑1 (MQ=255)          ggTATCAGATGCGGTAATCAGTGACGTTGAAAccc                      >  1:3905086/1‑35 (MQ=38)          ggTATCAGATGCGGTAATCAGTGACGTTGAAAccc                      >  1:7639064/1‑35 (MQ=255)           gTATCAGATGCGGTAATCAGTGACGTTGAAACCCa                     <  1:238572/35‑1 (MQ=255)           gTATCAGATGCGGTAATCAGTGACGTTGAAACCCa                     >  1:4610750/1‑35 (MQ=255)           gTATCAGATGCGGTAATCAGTGACGTTGAAACCCa                     <  1:1553608/35‑1 (MQ=255)           gTATCAGATGCGGTAATCAGTGACGTTGAAACCCa                     >  1:326880/1‑35 (MQ=255)           gTATCAGATGCGGTAATCAGTGACGTTGAAACCCa                     >  1:7273256/1‑35 (MQ=255)            tATCAGATGCGGTAATCAGTGACGTTGAAACCCag                    >  1:2255174/1‑35 (MQ=255)            tATCAGATGCGGTAATCAGTGACGTTGAAACCCag                    <  1:476061/35‑1 (MQ=38)            tATCAGATGCGGTAATCAGTGACGTTGAAACCCag                    <  1:1975908/35‑1 (MQ=38)            tATCAGATGCGGTAATCAGTGACGTTGAAACCCag                    <  1:7609655/35‑1 (MQ=38)            tATCAGATGCGGTAATCAGTGACGTTGAAACCCag                    <  1:6937892/35‑1 (MQ=38)            tATCAGATGCGGTAATCAGTGACGTTGAAACCCag                    <  1:5869128/35‑1 (MQ=38)            tATCAGATGCGGTAATCAGTGACGTTGAAACCCag                    <  1:3577155/35‑1 (MQ=38)            tATCAGATGCGGTAATCAGTGACGTTGAAACCCag                    <  1:3645677/35‑1 (MQ=255)              tCAGATGCGGTAATCAGTGACGTTGAAACCCagaa                  >  1:1625499/1‑35 (MQ=38)              tCAGATGCGGTAATCAGTGACGTTGAAACCCagaa                  <  1:6364533/35‑1 (MQ=38)              tCAGATGCGGTAATCAGTGACGTTGAAACCCagaa                  <  1:3537405/35‑1 (MQ=38)              tCAGATGCGGTAATCAGTGACGTTGAAACCCagaa                  >  1:2079888/1‑35 (MQ=38)               cAGATGCGGTAATCAGTGACGTTGAAACCCagaag                 <  1:1661101/35‑1 (MQ=38)                aGATGCGGTAATCAGTGACGTTGAAACCCagaaga                >  1:5715362/1‑35 (MQ=38)                aGATGCGGTAATCAGTGACGTTGAAACCCagaaga                >  1:7149720/1‑35 (MQ=255)                 gATGCGGTAATCAGTGACGTTGAAACCCAgaagaa               >  1:3538511/1‑35 (MQ=255)                 gATGCGGTAATCAGTGACGTTGAAACCCAgaagaa               >  1:2968788/1‑35 (MQ=38)                 gATGCGGTAATCAGTGACGTTGAAACCCAgaagaa               >  1:5223400/1‑35 (MQ=38)                  aTGCGGTAATCAGTGACGTTGAAACCCAGAAGAAt              <  1:5167608/35‑1 (MQ=38)                  aTGCGGTAATCAGTGACGTTGAAACCCAGAAGAAt              >  1:4645082/1‑35 (MQ=255)                  aTGCGGTAATCAGTGACGTTGAAACCCAGAAGAAt              <  1:355897/35‑1 (MQ=38)                   tGCGGTAATCAGTGACGTTGAAACCCAGAAGAATc             >  1:7499517/1‑35 (MQ=38)                   tGCGGTAATCAGTGACGTTGAAACCCAGAAGAATc             <  1:3376394/35‑1 (MQ=38)                   tGCGGTAATCAGTGACGTTGAAACCCAGAAGAATc             >  1:5327854/1‑35 (MQ=255)                    gCGGTAATCAGTGACGTTGAAACCCAGAAGAATcc            <  1:2317440/35‑1 (MQ=38)                     cGGTAATCAGTGACGTTGAAACCCAGAAGAATccc           <  1:1296653/35‑1 (MQ=38)                     cGGTAATCAGTGACGTTGAAACCCAGAAGAATccc           <  1:4381154/35‑1 (MQ=38)                      ggTAATCAGTGACGTTGAAACCCAGAAGAATCCCt          <  1:2382759/35‑1 (MQ=255)                      ggTAATCAGTGACGTTGAAACCCAGAAGAATCCCt          <  1:6441848/35‑1 (MQ=38)                       gTAATCAGTGACGTTGAAACCCAGAAGAATCCCTg         >  1:1459777/1‑35 (MQ=255)                       gTAATCAGTGACGTTGAAACCCAGAAGAATCCCTg         >  1:594282/1‑35 (MQ=38)                       gTAATCAGTGACGTTGAAACCCAGAAGAATCCCTg         >  1:2963297/1‑35 (MQ=255)                        tAATCAGTGACGTTGAAACCCAGAAGAATCCCTGc        >  1:5077766/1‑35 (MQ=38)                        tAATCAGTGACGTTGAAACCCAGAAGAATCCCTGc        <  1:2090123/35‑1 (MQ=255)                        tAATCAGTGACGTTGAAACCCAGAAGAATCCCTGc        <  1:3723311/35‑1 (MQ=255)                        tAATCAGTGACGTTGAAACCCAGAAGAATCCCTGc        <  1:3710271/35‑1 (MQ=255)                         aaTCAGTGACGTTGAAACCCAGAAGAATCCCTGCt       >  1:690411/1‑35 (MQ=255)                          aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTc      >  1:1128531/1‑35 (MQ=255)                          aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTc      >  1:5542112/1‑35 (MQ=38)                          aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTc      >  1:5029661/1‑35 (MQ=38)                          aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTc      >  1:7221173/1‑35 (MQ=255)                          aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTc      >  1:6985605/1‑35 (MQ=255)                           tCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCg     >  1:6215867/1‑35 (MQ=38)                           tCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCg     <  1:5848743/35‑1 (MQ=38)                           tCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCg     <  1:7818064/35‑1 (MQ=255)                            cAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt    <  1:7346659/35‑1 (MQ=255)                            cAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt    <  1:7681647/35‑1 (MQ=255)                             aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGtt   >  1:4414137/1‑35 (MQ=255)                             aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGtt   >  1:1831773/1‑35 (MQ=255)                              gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa  >  1:5927191/1‑35 (MQ=255)                              gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa  >  1:5321600/1‑35 (MQ=255)                              gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa  <  1:7654039/35‑1 (MQ=255)                              gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa  <  1:6857382/35‑1 (MQ=255)                                 |                                TGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTA  >  NC_000913/988467‑988530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑