Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,270,811	C→T	V347M (GTG→ATG)	chaA ←	Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,270,811	0	C	T	100.0%	279.4 / NA	67	V347M (GTG→ATG)	chaA	Na(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/40);  total (27/40)

AAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGG  >  NC_000913/1270779‑1270842                                 |                                aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGt                               <  1:6313791/35‑1 (MQ=255)  aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGtt                              >  1:7620788/1‑35 (MQ=38)   aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTa                             >  1:683304/1‑35 (MQ=255)   aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTa                             >  1:7690628/1‑35 (MQ=38)    cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                            <  1:741858/35‑1 (MQ=255)    cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                            >  1:4543014/1‑35 (MQ=38)    cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                            <  1:741926/35‑1 (MQ=255)    cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                            <  1:1720261/35‑1 (MQ=38)    cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                            <  1:4205974/35‑1 (MQ=38)    cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                            <  1:4616708/35‑1 (MQ=255)     aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAga                           >  1:6515875/1‑34 (MQ=38)     aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt                           >  1:1911100/1‑35 (MQ=38)     aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt                           <  1:2277040/35‑1 (MQ=255)     aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt                           <  1:5904545/35‑1 (MQ=38)     aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt                           <  1:2918887/35‑1 (MQ=255)     aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt                           <  1:2984719/35‑1 (MQ=38)      gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                          <  1:4184731/35‑1 (MQ=38)      gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                          >  1:5491466/1‑35 (MQ=255)      gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                          >  1:2366371/1‑35 (MQ=38)      gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                          <  1:1590653/35‑1 (MQ=38)       tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                         >  1:5178226/1‑35 (MQ=38)       tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                         <  1:923412/35‑1 (MQ=255)       tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                         <  1:6174699/35‑1 (MQ=38)         ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                       <  1:4348344/35‑1 (MQ=38)         ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                       >  1:597154/1‑35 (MQ=255)          cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                      >  1:7851288/1‑35 (MQ=255)          cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                      >  1:4776698/1‑35 (MQ=255)             aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                   >  1:571308/1‑35 (MQ=38)             aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                   >  1:1267376/1‑35 (MQ=255)             aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                   >  1:4795004/1‑35 (MQ=255)             aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                   >  1:3009806/1‑35 (MQ=255)              tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGt                  >  1:7721723/1‑35 (MQ=38)              tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGt                  >  1:4579836/1‑35 (MQ=255)              tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGt                  <  1:405897/35‑1 (MQ=38)              tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGt                  >  1:6171571/1‑35 (MQ=255)               gcgctgcgcCATTGAGCATGTTAGTACGTCCGGTg                 <  1:4761669/35‑1 (MQ=255)               gcgctgcgcCATTGAGCATGTTAGTACGTCCGGTg                 <  1:3347948/35‑1 (MQ=255)                cgctgcgcCATTGAGCATGTTAGTACGTCCGGTgg                <  1:964896/35‑1 (MQ=255)                 gctgcgcCATTGAGCATGTTAGTACGTCCGGTGga               <  1:7865382/35‑1 (MQ=255)                 gctgcgcCATTGAGCATGTTAGTACGTCCGGTGga               <  1:1799454/35‑1 (MQ=38)                    gcgcCATTGAGCATGTTAGTACGTCCGGTGGAGaa            <  1:7139109/35‑1 (MQ=255)                     cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAg           <  1:1115488/35‑1 (MQ=39)                     cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAg           <  1:7275451/35‑1 (MQ=255)                     cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAg           <  1:5094502/35‑1 (MQ=255)                       ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGga         <  1:6679846/35‑1 (MQ=255)                        cATTGAGCATGTTAGTACGTCCGGTGGAGAAGgag        <  1:5730501/35‑1 (MQ=255)                         aTTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga       >  1:3337704/1‑35 (MQ=39)                         aTTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga       <  1:2103584/35‑1 (MQ=255)                         aTTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga       <  1:6175180/35‑1 (MQ=255)                         aTTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga       >  1:1514633/1‑35 (MQ=39)                         aTTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga       <  1:4453801/35‑1 (MQ=255)                          ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGat      >  1:263408/1‑35 (MQ=39)                          ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGat      <  1:1980268/35‑1 (MQ=39)                           tGAGCATGTTAGTCCGTCCGGTGGAGAAGGAGata     <  1:4625844/35‑1 (MQ=25)                           tGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata     <  1:7473602/35‑1 (MQ=255)                           tGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata     <  1:6525280/35‑1 (MQ=255)                           tGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata     <  1:2845740/35‑1 (MQ=39)                           tGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata     <  1:6161027/35‑1 (MQ=255)                            gAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat    >  1:7627835/1‑35 (MQ=39)                            gAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat    >  1:1661528/1‑35 (MQ=255)                            gAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat    <  1:6405327/35‑1 (MQ=255)                            gAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat    <  1:5933953/35‑1 (MQ=255)                             aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg   >  1:6634209/1‑35 (MQ=39)                             aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg   >  1:3878198/1‑35 (MQ=255)                             aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg   >  1:5780023/1‑35 (MQ=39)                             aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg   <  1:993568/35‑1 (MQ=255)                              gCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg  <  1:7183786/35‑1 (MQ=255)                                 |                                AAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGG  >  NC_000913/1270779‑1270842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑