Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,742,755	G→C	P163R (CCG→CGG)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,742,755	0	G	C	100.0%	198.7 / NA	46	P163R (CCG→CGG)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (22/24);  total (22/24)

CTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCG  >  NC_000913/1742724‑1742785                                |                               cttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGaa                             <  1:4297722/35‑1 (MQ=255) cttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGaa                             >  1:4026518/1‑35 (MQ=255) cttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGaa                             >  1:206838/1‑35 (MQ=255)  ttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                            <  1:5948632/35‑1 (MQ=255)  ttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                            <  1:475094/35‑1 (MQ=255)  ttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                            <  1:6184566/35‑1 (MQ=255)  ttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                            <  1:4822040/35‑1 (MQ=255)   tcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                           <  1:5951993/35‑1 (MQ=255)   tcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                           <  1:4251977/35‑1 (MQ=255)    cccAAGCGTCGTGCGTTCCAGTGTTTCCCGAATTa                          >  1:3979926/1‑35 (MQ=37)     ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaa                         <  1:3007721/35‑1 (MQ=255)     ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaa                         <  1:3532517/35‑1 (MQ=255)      cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                        <  1:1839525/35‑1 (MQ=255)      cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                        <  1:661834/35‑1 (MQ=255)        agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                      >  1:3620113/1‑35 (MQ=39)        agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                      >  1:6041686/1‑35 (MQ=255)        agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                      >  1:4232566/1‑35 (MQ=255)           gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                   >  1:4668621/1‑35 (MQ=255)            tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                  <  1:493887/35‑1 (MQ=39)             cGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGc                 <  1:837620/35‑1 (MQ=255)             cGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGc                 >  1:3997801/1‑35 (MQ=255)               tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaa               <  1:6045299/35‑1 (MQ=255)               tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaa               <  1:38455/35‑1 (MQ=255)                gCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa              <  1:4266488/35‑1 (MQ=39)                gCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa              <  1:4498135/35‑1 (MQ=255)                gCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa              <  1:475004/35‑1 (MQ=255)                 cGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaa             >  1:4261712/1‑35 (MQ=255)                   ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc           >  1:1957916/1‑35 (MQ=255)                   ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc           >  1:1697075/1‑35 (MQ=255)                   ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc           >  1:7667910/1‑35 (MQ=255)                   ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc           >  1:5527340/1‑35 (MQ=255)                    tCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcg          <  1:7529981/35‑1 (MQ=255)                     ccAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc         >  1:849199/1‑35 (MQ=255)                     ccAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc         >  1:4170742/1‑35 (MQ=255)                     ccAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc         >  1:168982/1‑35 (MQ=255)                        gtgtTTCCCGAATTAAATGGACGCAAAAACGCGtt      <  1:5587793/35‑1 (MQ=38)                        gtgtTTCCCGAATTAAATGGACGCAAAAACGCGtt      <  1:5476781/35‑1 (MQ=38)                        gtgtTTCCCGAATTAAATGGACGCAAAAACGCGtt      >  1:547175/1‑35 (MQ=255)                        gtgtTTCCCGAATTAAATGGACGCAAAAACGCGtt      <  1:719358/35‑1 (MQ=255)                        gtgtTTCCCGAATTAAATGGACGCAAAAACGCGtt      >  1:7410643/1‑35 (MQ=38)                        gtgtTTCCCGAATTAAATGGACGCAAAAACGCGtt      >  1:1030683/1‑35 (MQ=255)                         tgtTTCCCGAATTAAATGGACGCAAAAACGCGTTg     <  1:1067326/35‑1 (MQ=38)                          gtTTCCCGAATTAAATGGACGCAAAAACGCGTTgg    >  1:4953412/1‑35 (MQ=255)                           tttCCCGAATTAAATGGACGCAAAAACGCGTTGGc   >  1:3361388/1‑35 (MQ=255)                           tttCCCGAATTAAATGGACGCAAAAACGCGTTGGc   >  1:3301758/1‑35 (MQ=38)                            ttCCCGAATTAAATGGACGCAAAAACGCGTTGGCg  <  1:1298702/35‑1 (MQ=38)                                |                               CTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCG  >  NC_000913/1742724‑1742785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑