Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,843,412	C→T	R12H (CGT→CAT)	hycH ←	formate hydrogenlyase assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,843,412	0	C	T	100.0%	264.2 / NA	65	R12H (CGT→CAT)	hycH	formate hydrogenlyase assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/33);  total (32/33)

CGGGCGTGGCATCGTTCTCATCAATAAATTTACGGCTCAGTTGACTGAACACCACCTTTTCAC  >  NC_000913/2843380‑2843442                                 |                               cgggcgTGGCATCGTTCTCATCAATAAATTTATgg                              <  1:6297893/35‑1 (MQ=255)   ggcgTGGCATCGTTCTCATCAATAAATTTATGGCt                            <  1:4405815/35‑1 (MQ=255)     cgTGGCATCGTTCTCATCAATAAATTTATGGCTCa                          >  1:4510780/1‑35 (MQ=255)      gTGGCATCGTTCTCATCAATAAATTTATGGCTCAg                         <  1:5281817/35‑1 (MQ=38)      gTGGCATCGTTCTCATCAATAAATTTATGGCTCAg                         <  1:7493141/35‑1 (MQ=38)       tGGCATCGTTCTCATCAATAAATTTATGGCTCAGt                        >  1:7123316/1‑35 (MQ=38)       tGGCATCGTTCTCATCAATAAATTTATGGCTCAGt                        <  1:2391919/35‑1 (MQ=255)       tGGCATCGTTCTCATCAATAAATTTATGGCTCAGt                        >  1:6149197/1‑35 (MQ=255)        ggCATCGTTCTCATCAATAAATTTATGGCTCAGtt                       <  1:1597301/35‑1 (MQ=255)        ggCATCGTTCTCATCAATAAATTTATGGCTCAGtt                       <  1:266965/35‑1 (MQ=38)        ggCATCGTTCTCATCAATAAATTTATGGCTCAGtt                       >  1:5170944/1‑35 (MQ=38)        ggCATCGTTCTCATCAATAAATTTATGGCTCAGtt                       <  1:608049/35‑1 (MQ=255)         gCATCGTTCTCATCAATAAATTTATGGCTCAGTTg                      >  1:4440528/1‑35 (MQ=38)         gCATCGTTCTCATCAATAAATTTATGGCTCAGTTg                      <  1:2680025/35‑1 (MQ=255)         gCATCGTTCTCATCAATAAATTTATGGCTCAGTTg                      >  1:917242/1‑35 (MQ=255)         gCATCGTTCTCATCAATAAATTTATGGCTCAGTTg                      >  1:930617/1‑35 (MQ=255)          cATCGTTCTCATCAATAAATTTATGGCTCAGTTGa                     >  1:5148602/1‑35 (MQ=255)           aTCGTTCTCATCAATAAATTTATGGCTCAGTTGAc                    <  1:2800646/35‑1 (MQ=255)           aTCGTTCTCATCAATAAATTTATGGCTCAGTTGAc                    <  1:2631984/35‑1 (MQ=38)           aTCGTTCTCATCAATAAATTTATGGCTCAGTTGAc                    <  1:4543444/35‑1 (MQ=38)            tCGTTCTCATCAATAAATTTATGGCTCAGTTGACt                   <  1:6701840/35‑1 (MQ=255)            tCGTTCTCATCAATAAATTTATGGCTCAGTTGACt                   <  1:7870632/35‑1 (MQ=255)             cGTTCTCATCAATAAATTTATGGCTCAGTTGACTg                  <  1:1930951/35‑1 (MQ=255)              gTTCTCATCAATAAATTTATGGCTCAGTTGACTGa                 >  1:2996614/1‑35 (MQ=38)              gTTCTCATCAATAAATTTATGGCTCAGTTGACTGa                 >  1:2792825/1‑35 (MQ=255)               ttCTCATCAATAAATTTATGGCTCAGTTGACTGaa                >  1:515313/1‑35 (MQ=38)               ttCTCATCAATAAATTTATGGCTCAGTTGACTGaa                >  1:6094863/1‑35 (MQ=38)               ttCTCATCAATAAATTTATGGCTCAGTTGACTGaa                >  1:7718303/1‑35 (MQ=255)                 ctcATCAATAAATTTATGGCTCAGTTGACTGAaca              >  1:4490723/1‑35 (MQ=255)                 ctcATCAATAAATTTATGGCTCAGTTGACTGAaca              >  1:4995896/1‑35 (MQ=255)                 ctcATCAATAAATTTATGGCTCAGTTGACTGAaca              >  1:847034/1‑35 (MQ=38)                 ctcATCAATAAATTTATGGCTCAGTTGACTGAaca              >  1:2041290/1‑35 (MQ=38)                  tcatcaATAAATTTATGGCTCAGTTGACTGAacac             <  1:5871668/35‑1 (MQ=255)                  tcatcaATAAATTTATGGCTCAGTTGACTGAacac             >  1:202145/1‑35 (MQ=255)                  tcatcaATAAATTTATGGCTCAGTTGACTGAacac             >  1:4230995/1‑35 (MQ=255)                   catcaATAAATTTATGGCTCAGTTGACTGAAcacc            >  1:140409/1‑35 (MQ=255)                    atcaATAAATTTATGGCTCAGTTGACTGAAcacca           >  1:5424021/1‑35 (MQ=255)                    atcaATAAATTTATGGCTCAGTTGACTGAAcacca           <  1:1926174/35‑1 (MQ=255)                    atcaATAAATTTATGGCTCAGTTGACTGAAcacca           <  1:4498098/35‑1 (MQ=255)                    atcaATAAATTTATGGCTCAGTTGACTGAAcacca           <  1:205935/35‑1 (MQ=255)                    atcaATAAATTTATGGCTCAGTTGACTGAAcacca           <  1:6245103/35‑1 (MQ=255)                    atcaATAAATTTATGGCTCAGTTGACTGAAcacca           >  1:2303664/1‑35 (MQ=255)                    atcaATAAATTTATGGCTCAGTTGACTGAAcacca           >  1:7107712/1‑35 (MQ=39)                    atcaATAAATTTATGGCTCAGTTGACTGAAcacca           >  1:7022949/1‑35 (MQ=39)                     tcaATAAATTTATGGCTCAGTTGACTGAAcaccac          <  1:5626600/35‑1 (MQ=39)                     tcaATAAATTTATGGCTCAGTTGACTGAAcaccac          >  1:6788436/1‑35 (MQ=39)                       aaTAAATTTATGGCTCAGTTGACTGAACACCACCt        <  1:2023237/35‑1 (MQ=255)                       aaTAAATTTATGGCTCAGTTGACTGAACACCACCt        <  1:7685939/35‑1 (MQ=255)                        aTAAATTTATGGCTCAGTTGGCTGAACACCACCtt       <  1:5880886/35‑1 (MQ=25)                        aTAAATTTATGGCTCAGTTGACTGAACACCACCtt       <  1:2655554/35‑1 (MQ=255)                        aTAAATTTATGGCTCAGTTGACTGAACACCACCtt       <  1:1278243/35‑1 (MQ=255)                        aTAAATTTATGGCTCAGTTGACTGAACACCACCtt       <  1:1650976/35‑1 (MQ=255)                        aTAAATTTATGGCTCAGTTGACTGAACACCACCtt       >  1:4417026/1‑35 (MQ=255)                        aTAAATTTATGGCTCAGTTGACTGAACACCACCtt       >  1:5672242/1‑35 (MQ=38)                        aTAAATTTATGGCTCAGTTGACTGAACACCACCtt       >  1:483093/1‑35 (MQ=38)                        aTAAATTTATGGCTCAGTTGACTGAACACCACCtt       <  1:4862323/35‑1 (MQ=255)                         tAAATTTATGGCTCAGTTGACTGAACACCACCttt      <  1:6950261/35‑1 (MQ=255)                         tAAATTTATGGCTCAGTTGACTGAACACCACCttt      >  1:4041301/1‑35 (MQ=38)                           aaTTTATGGCTCAGTTGACTGAACACCACCTTTTc    >  1:6634014/1‑35 (MQ=38)                           aaTTTATGGCTCAGTTGACTGAACACCACCTTTTc    <  1:4728041/35‑1 (MQ=255)                            aTTTATGGCTCAGTTGACTGAACACCACCTTTTCa   >  1:2004260/1‑35 (MQ=38)                             tttATGGCTCAGTTGACTGAACACCACCTTTTCAc  >  1:4920467/1‑35 (MQ=38)                             tttATGGCTCAGTTGACTGAACACCACCTTTTCAc  >  1:6643719/1‑35 (MQ=38)                             tttATGGCTCAGTTGACTGAACACCACCTTTTCAc  <  1:3838469/35‑1 (MQ=255)                             tttATGGCTCAGTTGACTGAACACCACCTTTTCAc  <  1:7653063/35‑1 (MQ=255)                             tttATGGCTCAGTTGACTGAACACCACCTTTTCAc  <  1:806433/35‑1 (MQ=38)                                 |                               CGGGCGTGGCATCGTTCTCATCAATAAATTTACGGCTCAGTTGACTGAACACCACCTTTTCAC  >  NC_000913/2843380‑2843442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑