Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,513,003	A→G	D212D (GAT→GAC)	trpS ←	tryptophan‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,513,003	0	A	G	100.0%	265.5 / NA	64	D212D (GAT→GAC)	trpS	tryptophan‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/40);  total (24/40)

GCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGAT  >  NC_000913/3512971‑3513034                                 |                                gCACGTTTGACTTTCTTCACTACCGATTTCGGGTc                               >  1:673588/1‑35 (MQ=37)  cACGTTTGATTTTCTTCACTACCGATTTCGGGTCt                              <  1:1561246/35‑1 (MQ=38)  cACGTTTGATTTTCTTCACTACCGATTTCGGGTCt                              >  1:3278013/1‑35 (MQ=38)    cGTTTGGTTTTCTTCACTACCGATTTCGGGTCTTc                            <  1:3881877/35‑1 (MQ=25)    cGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                            <  1:4654319/35‑1 (MQ=255)    cGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                            <  1:7732994/35‑1 (MQ=255)    cGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                            <  1:7479284/35‑1 (MQ=38)    cGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                            >  1:1512268/1‑35 (MQ=38)    cGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                            <  1:1571298/35‑1 (MQ=255)    cGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                            <  1:1675455/35‑1 (MQ=38)    cGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                            <  1:3111222/35‑1 (MQ=255)     gTTTGATTTTCTTCACTACCGATTTCGGGTCTTcc                           <  1:1600668/35‑1 (MQ=38)      tttGATTTTCTTCACTACCGATTTCGGGTCTTCca                          >  1:856802/1‑35 (MQ=38)      tttGATTTTCTTCACTACCGATTTCGGGTCTTCca                          >  1:4857794/1‑35 (MQ=38)       ttGATTTTCTTCACTACCGATTTCGGGTCTTCcag                         <  1:1951042/35‑1 (MQ=255)       ttGATTTTCTTCACTACCGATTTCGGGTCTTCcag                         <  1:7097031/35‑1 (MQ=255)        tGATTTTCTTCACTACCGATTTCGGGTCTTCcagc                        <  1:5172742/35‑1 (MQ=255)         gATTTTCTTCACTACCGATTTCGGGTCTTCcagca                       >  1:2859101/1‑35 (MQ=255)           ttttCTTCACTACCGATTTCGGGTCTTCCAGCAgg                     >  1:195047/1‑35 (MQ=38)            tttCTTCACTACCGATTTCGGGTCTTCCAGCAGGc                    <  1:5058227/35‑1 (MQ=38)            tttCTTCACTACCGATTTCGGGTCTTCCAGCAGGc                    <  1:4760023/35‑1 (MQ=38)             ttcttcACTACCGATTTCGGGTCTTCCAGCAGGcc                   >  1:6973696/1‑35 (MQ=255)              tcttcACTACCGATTTCGGGTCTTCCAGCAGGCCg                  >  1:3012014/1‑35 (MQ=255)               cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGa                 <  1:5543854/35‑1 (MQ=255)               cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGa                 <  1:5241025/35‑1 (MQ=38)               cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGa                 >  1:6025603/1‑35 (MQ=255)               cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGa                 <  1:7335648/35‑1 (MQ=255)               cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGa                 <  1:4067880/35‑1 (MQ=38)               cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGa                 >  1:416914/1‑35 (MQ=38)               cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGa                 <  1:73670/35‑1 (MQ=255)                ttcACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                <  1:864971/35‑1 (MQ=255)                ttcACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                >  1:5299365/1‑35 (MQ=255)                ttcACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                >  1:6796527/1‑35 (MQ=38)                ttcACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                <  1:1083651/35‑1 (MQ=38)                ttcACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                <  1:1260407/35‑1 (MQ=255)                ttcACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                <  1:194166/35‑1 (MQ=255)                ttcACTACCGATTTCGGGTCTTCCAGCACGCCGAt                >  1:5716303/1‑35 (MQ=37)                 tcACTACCGATTTCGGGTCTTCCAGCAGGCCGATa               <  1:7787390/35‑1 (MQ=38)                  cACTACCGATTTCGGGTCTTCCAGCAGGCCGATaa              >  1:1574605/1‑35 (MQ=38)                  cACTACCGATTTCGGGTCTTCCAGCAGGCCGATaa              >  1:2971760/1‑35 (MQ=255)                  cACTACCGATTTCGGGTCTTCCAGCAGGCCGATaa              <  1:636799/35‑1 (MQ=255)                   aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAAc             >  1:4522396/1‑35 (MQ=255)                    cTACCGATTTCGGGTCTTCCAGCAGGCCGATAACg            >  1:318850/1‑35 (MQ=38)                    cTACCGATTTCGGGTCTTCCAGCAGGCCGATAACg            <  1:6387005/35‑1 (MQ=255)                    cTACCGATTTCGGGTCTTCCAGCAGGCCGATAACg            >  1:1082207/1‑35 (MQ=255)                     tACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt           <  1:156257/35‑1 (MQ=255)                     tACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt           <  1:3089055/35‑1 (MQ=38)                     tACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt           <  1:1333099/35‑1 (MQ=38)                     tACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt           >  1:6025120/1‑35 (MQ=38)                     tACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt           <  1:6231780/35‑1 (MQ=255)                     tACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt           <  1:4442881/35‑1 (MQ=38)                       ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTa         >  1:3274374/1‑35 (MQ=38)                        cGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAt        <  1:3081650/35‑1 (MQ=255)                        cGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAt        <  1:2389934/35‑1 (MQ=38)                        cGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAt        <  1:4846613/35‑1 (MQ=255)                         gATTTCGGGTCTTCCAGCAGGCCGATAACGTTAtt       <  1:2266122/35‑1 (MQ=38)                          aTTTCGGGTCTTCCAGCAGGCCGATAACGTTATTg      <  1:565304/35‑1 (MQ=255)                          aTTTCGGGTCTTCCAGCAGGCCGATAACGTTATTg      >  1:5315172/1‑35 (MQ=255)                          aTTTCGGGTCTTCCAGCAGGCCGATAACGTTATTg      <  1:3459674/35‑1 (MQ=255)                          aTTTCGGGTCTTCCAGCAGGCCGATAACGTTATTg      >  1:4176501/1‑35 (MQ=38)                              cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt  <  1:6155687/35‑1 (MQ=255)                              cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt  <  1:5418962/35‑1 (MQ=255)                              cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt  >  1:5071370/1‑35 (MQ=255)                              cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt  <  1:4787036/35‑1 (MQ=255)                                 |                                GCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGAT  >  NC_000913/3512971‑3513034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑