Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,270,811	C→T	V347M (GTG→ATG)	chaA ←	Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,270,811	0	C	T	100.0%	255.4 / NA	68	V347M (GTG→ATG)	chaA	Na(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (45/23);  total (45/23)

ATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG  >  NC_000913/1270765‑1270857                                               |                                               atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              <  1:3895880/49‑1 (MQ=255)  tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              >  1:2224751/1‑48 (MQ=255)  tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTa                                            <  1:6110157/50‑1 (MQ=255)   caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                           >  1:3926780/1‑50 (MQ=255)   caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                           >  1:6150331/1‑50 (MQ=255)   caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                           >  1:490560/1‑50 (MQ=255)   caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                           >  1:2232646/1‑50 (MQ=255)   caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                           >  1:5751117/1‑50 (MQ=255)    aaaTAGGCGGCACACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                           >  1:4881755/1‑49 (MQ=38)    aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                           >  1:1849950/1‑49 (MQ=255)     aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                         <  1:3786206/50‑1 (MQ=255)     aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                         >  1:5975209/1‑50 (MQ=255)      aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                        <  1:5771930/50‑1 (MQ=255)       tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                       <  1:1135480/50‑1 (MQ=255)       tAGGCGGCAAACAGTGCCAGATGCCCTGCGCCATTGAGCATGTTAGTACg                                       >  1:5238149/1‑50 (MQ=38)        aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                      >  1:5548881/1‑50 (MQ=255)         ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                      <  1:957925/49‑1 (MQ=255)         ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                                     >  1:3972296/1‑50 (MQ=255)         ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                                     <  1:4217626/50‑1 (MQ=255)          gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc                                    >  1:1157364/1‑50 (MQ=255)          gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc                                    >  1:4023622/1‑50 (MQ=255)           cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                   >  1:2552390/1‑50 (MQ=255)           cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                   <  1:4549189/50‑1 (MQ=255)            ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                  >  1:1838781/1‑50 (MQ=255)            ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                  >  1:1524438/1‑50 (MQ=255)              cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                <  1:2846038/50‑1 (MQ=255)               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTgg                               <  1:5703334/50‑1 (MQ=255)               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:1437506/1‑49 (MQ=255)               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:3300215/1‑49 (MQ=255)                aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:816927/1‑48 (MQ=255)                aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:1504084/1‑48 (MQ=255)                aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGga                              <  1:1791652/50‑1 (MQ=255)                  cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  1:990531/1‑49 (MQ=255)                  cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  1:1429834/1‑49 (MQ=255)                   aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  1:6262831/1‑48 (MQ=255)                    gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          <  1:1405891/50‑1 (MQ=255)                    gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          <  1:1462958/50‑1 (MQ=255)                     tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAgg                         <  1:193939/50‑1 (MQ=255)                     tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:4746622/1‑49 (MQ=255)                      gCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:4108426/1‑48 (MQ=255)                       ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:2921747/1‑47 (MQ=255)                       ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGga                        <  1:5982253/49‑1 (MQ=255)                       ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGga                        <  1:922257/49‑1 (MQ=255)                        cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:1998042/1‑46 (MQ=255)                         aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga                      >  1:2372345/1‑49 (MQ=255)                           aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                   >  1:3945777/1‑50 (MQ=255)                            tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg                  >  1:909315/1‑50 (MQ=255)                            tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg                  <  1:4313562/50‑1 (MQ=255)                             gcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                 >  1:3830143/1‑50 (MQ=255)                              cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                 >  1:4477060/1‑49 (MQ=255)                              cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                 <  1:3981398/49‑1 (MQ=255)                                 tgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  1:664034/1‑48 (MQ=255)                                 tgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  1:2905418/1‑48 (MQ=255)                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca             <  1:742918/48‑1 (MQ=255)                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca             <  1:1198060/48‑1 (MQ=255)                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg            >  1:5037709/1‑49 (MQ=255)                                    gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa          >  1:2283663/1‑50 (MQ=255)                                     ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGcgc         <  1:2028026/50‑3 (MQ=255)                                      cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  1:2148702/1‑49 (MQ=255)                                      cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  1:5985144/1‑49 (MQ=255)                                      cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  1:5360050/1‑49 (MQ=255)                                      cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  1:3060037/1‑49 (MQ=255)                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa     >  1:1020221/1‑48 (MQ=255)                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag    <  1:1204484/49‑1 (MQ=255)                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag    <  1:5155351/49‑1 (MQ=255)                                            gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag  >  1:6089581/1‑50 (MQ=255)                                            gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag  >  1:3879242/1‑50 (MQ=255)                                            gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag  >  1:2923093/1‑50 (MQ=255)                                               |                                               ATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG  >  NC_000913/1270765‑1270857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑