| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,440,194 | C→A | G279V (GGT→GTT) | rpoA ← | RNA polymerase subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,440,194 | 0 | C | A | 80.8% | 185.9 / 50.4 | 78 | G279V (GGT→GTT) | rpoA | RNA polymerase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (6/9); new base A (32/31); total (38/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
AAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGT > NC_000913/3440145‑3440243 | aaGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAc > 1:269075/1‑50 (MQ=255)aaGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAc < 1:2947931/50‑1 (MQ=255) aGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAcc < 1:1047779/50‑1 (MQ=255) gTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGa > 1:1610622/1‑50 (MQ=255) gTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGa > 1:2401651/1‑50 (MQ=255) gTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGa < 1:2520846/50‑1 (MQ=255) gTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGa > 1:1426212/1‑50 (MQ=255) ttAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGat < 1:4060046/50‑1 (MQ=255) ttAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGa > 1:2578839/1‑49 (MQ=255) ttAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGat < 1:2308582/50‑1 (MQ=255) ttAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGat < 1:3933128/50‑1 (MQ=255) tAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGata > 1:5201389/1‑50 (MQ=255) aGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGata > 1:423221/1‑49 (MQ=255) aGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGata < 1:3772685/49‑1 (MQ=255) ggCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGatat < 1:2855729/49‑1 (MQ=255) cGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGt > 1:4581669/1‑50 (MQ=255) cGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGt < 1:5196087/50‑1 (MQ=255) cGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGt < 1:2062508/50‑1 (MQ=255) gTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTg > 1:3686944/1‑50 (MQ=255) gTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTg > 1:5386534/1‑50 (MQ=255) gTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTg < 1:5503806/50‑1 (MQ=255) gTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTg > 1:5545065/1‑50 (MQ=255) gTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTg > 1:1049478/1‑50 (MQ=255) ttttAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTg > 1:4707780/1‑49 (MQ=255) ttAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGa > 1:1356588/1‑49 (MQ=255) tAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGAt < 1:1851283/49‑1 (MQ=255) aaGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATa < 1:2573761/49‑1 (MQ=255) aGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAg < 1:4718373/49‑1 (MQ=255) aGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGc < 1:5408172/50‑1 (MQ=255) aGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGc > 1:6361122/1‑50 (MQ=255) aGGAGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGc > 1:187039/1‑50 (MQ=255) aGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTc > 1:497383/1‑50 (MQ=255) aGCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTc > 1:1078411/1‑50 (MQ=255) gCTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCt < 1:6103968/50‑1 (MQ=255) cTCAACCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTg < 1:4443876/50‑1 (MQ=255) aaCCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCtt < 1:626747/50‑1 (MQ=255) aaCCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCtt > 1:4658199/1‑50 (MQ=255) aaCCTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCtt > 1:2499741/1‑50 (MQ=255) ccTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTa < 1:1263981/50‑1 (MQ=255) ccTCGGTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTa < 1:4800134/50‑1 (MQ=255) tCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAg > 1:6174909/1‑50 (MQ=255) ggTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAgg < 1:6121029/49‑1 (MQ=255) ggTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAg < 1:638838/48‑1 (MQ=255) ggTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGc < 1:5639481/50‑1 (MQ=255) ggTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAgg > 1:142996/1‑49 (MQ=255) ggTACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGc < 1:1923795/50‑1 (MQ=255) tACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAg > 1:1278672/1‑50 (MQ=255) tACGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAg > 1:6321962/1‑50 (MQ=255) aCGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGt > 1:2174401/1‑50 (MQ=255) cGCTGTTCCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAAtt < 1:2259806/50‑1 (MQ=255) cGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGt < 1:4016713/49‑1 (MQ=255) cGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGt < 1:5516540/49‑1 (MQ=255) gCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGt > 1:4837679/1‑48 (MQ=255) gCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGt > 1:1877083/1‑48 (MQ=255) gCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTa < 1:4113411/50‑1 (MQ=255) cTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAg < 1:4542795/50‑1 (MQ=255) cTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAg > 1:4968058/1‑50 (MQ=255) cTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAg > 1:3511194/1‑50 (MQ=255) cTGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAg < 1:1343622/50‑1 (MQ=255) tGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAg > 1:5876999/1‑49 (MQ=255) tGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAg > 1:5765301/1‑49 (MQ=255) tGTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAg > 1:1537089/1‑49 (MQ=255) gTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAg > 1:4461545/1‑48 (MQ=255) gTACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAg > 1:4671840/1‑48 (MQ=255) tACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAg > 1:1848348/1‑47 (MQ=255) tACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGc < 1:638323/48‑1 (MQ=255) tACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGc < 1:3483105/48‑1 (MQ=255) tACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGc < 1:1533796/48‑1 (MQ=255) tACCAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCa < 1:2705010/49‑1 (MQ=255) ccAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCaga < 1:5198128/49‑1 (MQ=255) ccAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCaga < 1:4331301/49‑1 (MQ=255) ccAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCaga < 1:1484467/49‑1 (MQ=255) ccAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCaga < 1:319268/49‑1 (MQ=255) ccAGATCAACGATATAGCGGATAGCTTCTGCTTTAAGGCAGTTAGCagag > 1:3282933/1‑50 (MQ=255) cAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGc < 1:6411879/50‑1 (MQ=255) cAGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGc > 1:3306395/1‑50 (MQ=255) aGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCg < 1:3270978/50‑1 (MQ=255) aGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCg > 1:2634214/1‑50 (MQ=255) aGATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCg < 1:3931877/50‑1 (MQ=255) gATCAACGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCg > 1:5864609/1‑49 (MQ=255) aCCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAg < 1:162095/50‑1 (MQ=255) ccGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGt > 1:1625133/1‑50 (MQ=255) | AAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGT > NC_000913/3440145‑3440243 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |