Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,477,150	C→T	K90K (AAG→AAA)	fkpA ←	peptidyl‑prolyl cis‑trans isomerase FkpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,477,150	0	C	T	100.0%	321.1 / NA	79	K90K (AAG→AAA)	fkpA	peptidyl‑prolyl cis‑trans isomerase FkpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/51);  total (28/51)

CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAT  >  NC_000913/3477104‑3477196                                               |                                               cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt                                              <  1:3540544/49‑1 (MQ=255) cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt                                              <  1:6359990/49‑1 (MQ=255) cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                             >  1:2207630/1‑50 (MQ=255) cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                             >  1:1867039/1‑50 (MQ=255)  gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            <  1:3404869/50‑1 (MQ=255)  gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  1:6057201/1‑50 (MQ=255)  gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  1:2164847/1‑50 (MQ=255)     tGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                         <  1:6400718/50‑1 (MQ=255)     tGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                         <  1:3566998/50‑1 (MQ=255)       cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                        >  1:2090472/1‑49 (MQ=255)       cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                        <  1:3413036/49‑1 (MQ=255)        ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                       >  1:4601084/1‑49 (MQ=255)         tGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                     >  1:5678470/1‑50 (MQ=255)          gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                     <  1:935344/49‑1 (MQ=255)          gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaa                                      <  1:285829/48‑1 (MQ=255)          gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaa                                      <  1:1053761/48‑1 (MQ=255)          gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                    >  1:548119/1‑50 (MQ=255)           tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                    <  1:3963433/49‑1 (MQ=255)           tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                    <  1:6244231/49‑1 (MQ=255)           tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                    <  1:5295861/49‑1 (MQ=255)           tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                   >  1:2270697/1‑50 (MQ=255)             gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGc                                  <  1:1116924/49‑1 (MQ=255)             gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCa                                 >  1:3194565/1‑50 (MQ=255)              agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                                <  1:6388540/50‑1 (MQ=255)              agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                                <  1:2805013/50‑1 (MQ=255)               gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc                               <  1:2189149/50‑1 (MQ=255)                tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATcc                              <  1:706017/50‑1 (MQ=255)                   gTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                            >  1:3436957/1‑49 (MQ=255)                    ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             <  1:309926/47‑1 (MQ=255)                    ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                            <  1:5838686/48‑1 (MQ=255)                    ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                            <  1:5685796/48‑1 (MQ=255)                    ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                            <  1:2015722/48‑1 (MQ=255)                    ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                          <  1:2049195/50‑1 (MQ=255)                    ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGa                           <  1:3312256/49‑1 (MQ=255)                      cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAac                         <  1:5447223/49‑1 (MQ=255)                       gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                        <  1:6382508/49‑1 (MQ=255)                       gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                        <  1:1857473/49‑1 (MQ=255)                       gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                        <  1:2194717/49‑1 (MQ=255)                        aTCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  1:3712973/1‑49 (MQ=255)                        aTCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  1:4204254/1‑49 (MQ=255)                           tctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   >  1:4638395/1‑50 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       <  1:3700549/44‑1 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       <  1:4851244/44‑1 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                    <  1:4157321/47‑1 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                    <  1:756555/47‑1 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                    <  1:4656362/47‑1 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   <  1:5976534/48‑1 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   <  1:5886782/48‑1 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   <  1:1753900/48‑1 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   <  1:4756524/48‑1 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAACGCATCCTGCACACCAGCGa                 >  1:3379665/1‑50 (MQ=255)                              tGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt                >  1:4835086/1‑50 (MQ=255)                              tGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt                >  1:3708296/1‑50 (MQ=255)                               ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCg                  <  1:1780329/47‑1 (MQ=255)                               ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCg                  <  1:2209673/47‑1 (MQ=255)                               ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCg                  <  1:5791072/47‑1 (MQ=255)                                gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa              <  1:4514367/50‑1 (MQ=255)                                gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa              <  1:6034640/50‑1 (MQ=255)                                 tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg             >  1:1672413/1‑50 (MQ=255)                                 tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg             >  1:155230/1‑50 (MQ=255)                                 tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg             <  1:2380936/50‑1 (MQ=255)                                   ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            <  1:4500078/49‑1 (MQ=255)                                   ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            <  1:3824435/49‑1 (MQ=255)                                   ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:94735/1‑50 (MQ=255)                                   ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           <  1:3361760/50‑1 (MQ=255)                                    gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:2520311/1‑49 (MQ=255)                                    gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg          <  1:5031459/50‑1 (MQ=255)                                     agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa         <  1:1125250/50‑1 (MQ=255)                                     agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa         >  1:868268/1‑50 (MQ=255)                                       agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc       >  1:5488210/1‑50 (MQ=255)                                       agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc       >  1:6265394/1‑50 (MQ=255)                                       agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc       >  1:3050958/1‑50 (MQ=255)                                        gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      >  1:3948113/1‑50 (MQ=255)                                        gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      <  1:3840580/50‑1 (MQ=255)                                        gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      <  1:6404888/50‑1 (MQ=255)                                        gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      <  1:2862042/50‑1 (MQ=255)                                        gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      <  1:798077/50‑1 (MQ=255)                                         tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt     <  1:1033492/50‑1 (MQ=255)                                           tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt    <  1:4934012/49‑1 (MQ=255)                                           tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa   >  1:4215987/1‑50 (MQ=255)                                            gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAt  >  1:5402415/1‑50 (MQ=255)                                               |                                               CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAT  >  NC_000913/3477104‑3477196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑