breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 24 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,423,2870GC38.1% 31.5 / 10.0 21intergenic (+93/+136)yhdZ/rrfFputative ABC transporter ATP‑binding subunit YhdZ/5S ribosomal RNA
*NC_000913599,5760TG37.0% 95.9 / 16.4 54V288G (GTG→GGG) cusAcopper/silver export system RND permease
*NC_000913888,0140TG34.6% 95.3 / 13.1 52noncoding (44/81 nt)rybBsmall regulatory RNA RybB
*NC_0009134,070,4620TC34.6% 51.9 / 54.1 52intergenic (‑61/+53)yihR/yihSputative aldose 1‑epimerase YihR/sulfoquinovose isomerase
*NC_0009131,811,9330GC33.9% 101.5 / 67.4 62A334P (GCC→CCC) tcyPcystine/sulfocysteine:cation symporter
*NC_0009134,296,0600CT32.8% 223.5 / 186.0 171intergenic (+266/+376)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009133,097,4670TG31.0% 121.6 / 21.4 58G67G (GGT→GGGyggWputative coproporphyrinogen‑III oxidase‑like protein YggW
*NC_0009131,911,7500TG30.2% 118.5 / 10.9 53H276P (CAC→CCC) htpXzinc dependent endoprotease
*NC_0009133,829,2330CA28.3% 139.5 / 45.4 60L97M (CTG→ATG) xanPxanthine:H(+) symporter XanP
*NC_0009133,556,2070TC25.0% 112.3 / 12.1 48S215G (AGT→GGT) rtcARNA 3'‑terminal phosphate cyclase
*NC_0009132,892,1950AT24.4% 106.9 / 14.8 45intergenic (‑297/‑19)cysJ/queDsulfite reductase, flavoprotein subunit/6‑carboxy‑5,6,7,8‑tetrahydropterin synthase
*NC_000913213,3410AG24.1% 128.4 / 13.8 54G337G (GGA→GGGtilStRNA(Ile)‑lysidine synthetase
*NC_0009133,862,6610TG23.9% 155.3 / 24.6 72T315P (ACC→CCC) glvCputative PTS enzyme II component GlvC
*NC_0009134,457,1050AG23.8% 152.9 / 15.7 63I441V (ATC→GTC) mplUDP‑N‑acetylmuramate‑‑L‑alanyl‑gamma‑D‑glutamyl‑ meso‑2,6‑diaminoheptanedioate ligase
*NC_00091373,4130AC23.4% 102.0 / 12.6 47V370G (GTG→GGG) thiPthiamine ABC transporter membrane subunit
*NC_0009133,850,8910GA23.3% 191.1 / 58.8 86Q68* (CAA→TAA) ilvNacetohydroxy acid synthase I subunit IlvN
*NC_0009133,342,8880TG23.1% 138.5 / 14.6 52V18G (GTG→GGG) lptClipopolysaccharide transport system protein LptC
*NC_000913176,2740GT22.6% 131.8 / 12.2 53A390S (GCT→TCT) clcAchloride:H(+) antiporter ClcA
*NC_0009132,483,2680TA22.4% 130.1 / 12.0 49L24F (TTA→TTTemrKtripartite efflux pump membrane fusion protein EmrK
*NC_0009132,185,1990TG21.8% 229.0 / 17.6 80T35P (ACC→CCC) thiMhydroxyethylthiazole kinase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 284630058 (0.700)16 (0.210) 15/92 3.5 22.8% coding (172/1710 nt) hycE formate hydrogenlyase subunit HycE
?NC_000913 2867269 = 54 (0.690)coding (283/993 nt) rpoS RNA polymerase, sigma S (sigma 38) factor
* ? NC_000913 = 325632283 (1.000)3 (0.050) 3/76 7.9 4.7% coding (330/1311 nt) cyuA putative L‑cysteine desulfidase CyuA
?NC_000913 3256551 = 58 (0.910)coding (101/1311 nt) cyuA putative L‑cysteine desulfidase CyuA