Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	765,191	Δ1 bp	coding (685/723 nt)	mngR ←	DNA‑binding transcriptional repressor MngR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	765,189	0	T	.	100.0%	280.7 / NA	58	coding (687/723 nt)	mngR	DNA‑binding transcriptional repressor MngR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (33/25);  total (33/25)

GCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACC  >  NC_000913/765140‑765234                                                  |                                              gCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                               >  1:5059442/1‑50 (MQ=255)  ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                               >  1:1488365/1‑49 (MQ=255)  ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                               >  1:615062/1‑49 (MQ=255)   cTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                             >  1:345880/1‑49 (MQ=255)   cTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                             <  1:5551459/50‑2 (MQ=255)    ttttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                             >  1:306209/1‑48 (MQ=255)    ttttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                             >  1:4942617/1‑48 (MQ=255)         tgtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCa                                      >  1:1674698/1‑50 (MQ=39)          gtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCa                                      <  1:4504817/49‑1 (MQ=38)          gtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCa                                      <  1:537024/49‑1 (MQ=38)          gtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                                     >  1:1420691/1‑50 (MQ=39)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                                     <  1:557532/49‑1 (MQ=38)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                    >  1:1866676/1‑50 (MQ=39)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                    <  1:4748343/50‑1 (MQ=39)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                    <  1:1141094/50‑1 (MQ=39)             cTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                  >  1:4329783/1‑50 (MQ=39)             cTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                  >  1:1468729/1‑50 (MQ=39)             cTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                  <  1:3615368/50‑1 (MQ=39)               tATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTa                                >  1:234909/1‑50 (MQ=39)                  cgcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             >  1:2393226‑M1/1‑50 (MQ=255)                   gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             >  1:1127756‑M1/1‑49 (MQ=255)                    cgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             >  1:5347452‑M1/1‑48 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:4358929‑M1/1‑47 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:5450567‑M1/1‑47 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:1917421‑M1/1‑47 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          <  1:4046313‑M1/50‑4 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          <  1:2599811‑M1/50‑4 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:3115413‑M1/1‑47 (MQ=255)                      atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:2913195‑M1/1‑46 (MQ=255)                      atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:4778865‑M1/1‑46 (MQ=255)                       tgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagca                        <  1:5243260‑M1/50‑6 (MQ=255)                         atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaa                       <  1:2481597‑M1/49‑7 (MQ=255)                         atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaa                       <  1:2001335‑M1/49‑7 (MQ=255)                          ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatc                     <  1:4164400‑M1/50‑9 (MQ=255)                            ttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatca                    <  1:3209115‑M1/49‑10 (MQ=255)                            ttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatca                    <  1:5349073‑M1/49‑10 (MQ=255)                              cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac                 >  1:2749404‑M1/1‑38 (MQ=255)                              cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa                  <  1:4704179‑M1/49‑12 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc                >  1:47504‑M1/1‑37 (MQ=255)                                  gcgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccg               >  1:3142882‑M1/1‑34 (MQ=255)                                   cgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaag            >  1:3784347‑M1/1‑33 (MQ=255)                                   cgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaa             <  1:522007‑M1/49‑17 (MQ=255)                                    gcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc           >  1:2734034‑M1/1‑32 (MQ=255)                                    gcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc           >  1:2658855‑M1/1‑32 (MQ=255)                                     cTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcc          <  1:678448‑M1/50‑20 (MQ=255)                                     cTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcc          <  1:2683324‑M1/50‑20 (MQ=255)                                     cTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcc          <  1:2874113‑M1/50‑20 (MQ=255)                                      tATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca         >  1:1841516‑M1/1‑30 (MQ=255)                                      tATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca         >  1:544043‑M1/1‑30 (MQ=255)                                      tATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca         >  1:5315378‑M1/1‑30 (MQ=255)                                       aTCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca         >  1:1081729‑M1/1‑29 (MQ=255)                                       aTCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca         >  1:4059076‑M1/1‑29 (MQ=255)                                       aTCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca         >  1:4415308‑M1/1‑29 (MQ=255)                                        tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccaca       >  1:4505785‑M1/1‑28 (MQ=255)                                        tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca         <  1:1509033‑M1/48‑21 (MQ=255)                                         cAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccaca       >  1:3238584‑M1/1‑27 (MQ=255)                                         cAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccaca       >  1:4313094‑M1/1‑27 (MQ=255)                                          aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacata     <  1:4145941‑M1/50‑25 (MQ=255)                                          aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacata     >  1:1885690‑M1/1‑26 (MQ=255)                                          aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacat      <  1:2036270‑M1/49‑24 (MQ=255)                                          aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacat      <  1:167757‑M1/49‑24 (MQ=255)                                           gggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatat    >  1:1533574‑M1/1‑25 (MQ=255)                                            ggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatg   <  1:3838335‑M1/50‑27 (MQ=255)                                            ggTAAA‑TTATAGTCATCGGTATTAtagaaatcaaaccgaagccacatatg   <  1:4629359‑M1/50‑27 (MQ=255)                                             gTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatgc  >  1:3576397‑M1/1‑23 (MQ=255)                                                  |                                              GCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACC  >  NC_000913/765140‑765234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑