| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,816,579 | 0 | G | A | 41.5% | 40.0 / 91.1 | 65 | intergenic (‑30/‑97) | rph/yicC | truncated RNase PH/UPF0701 family protein YicC |
| Reads supporting (aligned to +/- strand): ref base G (20/18); new base A (13/14); total (33/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGTTATTCCTATCCTGACAAGGCA > NC_000913/3816532‑3816627 | aCGGCCTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCt > 1:1644746/1‑50 (MQ=255) cGGCCTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTg > 1:1317543/1‑50 (MQ=255) cGGCCTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGACt < 1:3322861/49‑1 (MQ=255) ggCCTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGACTgc < 1:4947512/50‑1 (MQ=255) ccTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTgcg > 1:340429/1‑49 (MQ=255) ccTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGACTgcg > 1:3188675/1‑49 (MQ=255) tgctgGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCAt > 1:3689746/1‑50 (MQ=255) tgctgGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCAt > 1:2086203/1‑50 (MQ=255) ctgGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCAt < 1:1523636/48‑1 (MQ=255) ctgGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCAt < 1:1279982/48‑1 (MQ=255) ctgGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCATta < 1:4933239/50‑1 (MQ=255) gACGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATtat < 1:3619955/48‑1 (MQ=255) gACGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATTATAc > 1:4563396/1‑50 (MQ=255) gACGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATTATAc > 1:653583/1‑50 (MQ=255) gACGCATATTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCATTATAc < 1:2240549/50‑1 (MQ=255) aCGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATTATACg < 1:580633/50‑1 (MQ=255) aCGCATATTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCATTATACg < 1:571628/50‑1 (MQ=255) cGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATTATACgg < 1:4350924/50‑1 (MQ=255) cGCATATTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCATTATACgg < 1:622853/50‑1 (MQ=255) gCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATTATACGGa > 1:2060058/1‑50 (MQ=255) gCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATTATACGGa < 1:1307037/50‑1 (MQ=255) gCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATTATACGGa < 1:3508960/50‑1 (MQ=255) gCATATTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCATTATACGGa > 1:462795/1‑50 (MQ=255) gCATATTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCATTATACGGa > 1:3407102/1‑50 (MQ=255) cATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATTATACGGAc > 1:542416/1‑50 (MQ=255) cATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATTATACGGAc < 1:1410817/50‑1 (MQ=255) cATATTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCATTATACGGAc > 1:4039646/1‑50 (MQ=255) atatTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCATTATACGGACt < 1:179509/50‑1 (MQ=255) atatTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCATTATACGGACt < 1:3977885/50‑1 (MQ=255) tatTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATTATACGGACtt < 1:2165455/50‑1 (MQ=255) tatTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCATTATACGGACtt < 1:5170343/50‑1 (MQ=255) tatTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCATTATACGGACtt < 1:2623119/50‑1 (MQ=255) ttGAAATCTCCGGCTTGAAACAAATGTGACTGCGCATTATACGGACTTcc < 1:645684/50‑1 (MQ=255) aaaTCTCCGGCTTGAAACAAATGTGGCTGCGCATTATACGGACTTCcggc < 1:2066243/50‑1 (MQ=255) aaaTCTCCGGCTTGAAACAAATGTGGCTGCGCATTATACGGACTTCcggc > 1:4836613/1‑50 (MQ=255) aaaTCTCCGGCTTGAAACAAATGTGGCTGCGCATTATACGGACTTCcggc < 1:582648/50‑1 (MQ=255) aaTCTCCGGCTTGAAACAAATGTGGCTGCGCATTATACGGACTTCcggcg < 1:3984862/50‑1 (MQ=255) aaTCTCCGGCTTGAAACAAATGTGACTGCGCATTATACGGACTTCcggcg > 1:5585833/1‑50 (MQ=255) aTCTCCGGCTTGAAACAAATGTGGCTGCGCATTATACGGACTTCcggcgg < 1:3893766/50‑1 (MQ=255) tctcCGGCTTGAAACAAATGTGACTGCGCATTATACGGACTTCCGGCGGt > 1:3010226/1‑50 (MQ=255) ctcCGGCTTGAAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGt > 1:3548549/1‑49 (MQ=255) tcCGGCTTGAAACAGATGTGACTGCGCATTATACGGACTTCCGGCGGTTa > 1:2608557/1‑50 (MQ=255) ccGGCTTGAAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGTTAt > 1:4806324/1‑50 (MQ=255) ccGGCTTGAAACAAATGTGACTGCGCATTATACGGACTTCCGGCGGTTAt > 1:2577059/1‑50 (MQ=255) ccGGCTTGAAACAAATGTGACTGCGCATTATACGGACTTCCGGCGGTTAt > 1:3470159/1‑50 (MQ=255) cGGCTTGAAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGTTa < 1:3648159/48‑1 (MQ=255) cGGCTTGAAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGTTAtt > 1:132897/1‑50 (MQ=255) cGGCTTGAAACAAATGTGACTGCGCATTATACGGACTTCCGGCGGTTAt < 1:659679/49‑1 (MQ=255) ggCTTGAAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGTTATTc > 1:3112189/1‑50 (MQ=255) gCTTGAAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGTTATTcc > 1:247127/1‑50 (MQ=255) gCTTGAAACAAATGTGACTGCGCATTATACGGACTTCCGGCGGTTATTcc > 1:2569298/1‑50 (MQ=255) gCTTGAAACAAATGTGACTGCGCATTATACGGACTTCCGGCGGTTATTcc > 1:1028641/1‑50 (MQ=255) cTTGAAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGTTATTcc > 1:390315/1‑49 (MQ=255) cTTGAAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGTTATTcc > 1:3835787/1‑49 (MQ=255) tGAAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGTTATTCCTAt > 1:3054132/1‑50 (MQ=255) gAAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGTTATTCCTAt < 1:1875294/49‑1 (MQ=255) aaaCAAATGTGGCTGCGCATTATACGGACTTCCGGCGGTTATTCCTATc < 1:578669/49‑1 (MQ=255) aaCAAATGTGACTGCGCATTATACGGACTTCCGGCGGTTATTCCTATCCt < 1:357794/50‑1 (MQ=255) aaCAAATGTGACTGCGCATTATACGGACTTCCGGCGGTTATTCCTATCCt > 1:2658205/1‑50 (MQ=255) aCAAATGTGGCTGCGCATTATACGGACTTCCGGCGGTTATTCCTATCCTg < 1:784736/50‑1 (MQ=255) aaaTGTGGCTGCGCATTATACGGACTTCCGGCGGTTATTCCTATCCTGa > 1:807173/1‑49 (MQ=255) aaTGTGGCTGCGCATTATACGGACTTCCGGCGGTTATTCCTATCCTGACa < 1:3879966/50‑1 (MQ=255) aTGTGACTGCGCATTATACGGACTTCCGGCGGTTATTCCTATCCTGAc > 1:5369526/1‑48 (MQ=255) tgtgGCTGCGCATTATACGGACTTCCGGCGGTTATTCCTATCCTGACAAg > 1:5551173/1‑50 (MQ=255) tgGCTGCGCATTATACGGACTTCCGGCGGTTATTCCTATCCTGACAAGGc < 1:1803935/50‑1 (MQ=255) ggCTGCGCATTATACGGACTTCCGGCGGTTATTCCTATCCTGACAAGGCa > 1:5192156/1‑50 (MQ=255) | ACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGTTATTCCTATCCTGACAAGGCA > NC_000913/3816532‑3816627 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |