Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	988,499	G→A	P163S (CCA→TCA)	asnS ←	asparagine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	988,499	0	G	A	100.0%	228.7 / NA	59	P163S (CCA→TCA)	asnS	asparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/35);  total (24/35)

GCGGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAGC  >  NC_000913/988452‑988545                                                |                                               gcgGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACg                                              <  1:587508/50‑1 (MQ=255)   ggAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                            <  1:2943778/50‑1 (MQ=255)    gAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                            >  1:4048416/1‑49 (MQ=255)    gAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTg                                           <  1:2582330/50‑1 (MQ=255)     aaCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTg                                           >  1:3252549/1‑49 (MQ=255)     aaCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTg                                           >  1:7242/1‑49 (MQ=255)      aCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaa                                         <  1:4269680/50‑1 (MQ=255)      aCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaa                                         <  1:4412495/50‑1 (MQ=255)      aCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGa                                          >  1:1293297/1‑49 (MQ=255)       cATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaaa                                        <  1:1287096/50‑1 (MQ=255)        aTTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAc                                       >  1:4148053/1‑50 (MQ=255)        aTTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAc                                       >  1:1778108/1‑50 (MQ=255)         tttCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                      >  1:4078413/1‑50 (MQ=255)         tttCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                      <  1:4096552/50‑1 (MQ=255)         tttCGCCTGCACCGTCGGTATCAGATGCGGTAATCAGGGACGTTGAAAcc                                      >  1:5303925/1‑50 (MQ=255)           tCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                      >  1:4711602/1‑48 (MQ=255)            cGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                      >  1:4522937/1‑47 (MQ=255)            cGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCa                                    <  1:4948449/49‑1 (MQ=255)             gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCaga                                  <  1:5563006/50‑1 (MQ=255)             gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCaga                                  <  1:762515/50‑1 (MQ=255)             gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                   <  1:1921290/49‑1 (MQ=255)              ccTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaa                                 <  1:1744072/50‑1 (MQ=255)               cTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaag                                <  1:220975/50‑1 (MQ=255)                   aCCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATc                            >  1:3813243/1‑50 (MQ=255)                   aCCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATc                            >  1:4687321/1‑50 (MQ=255)                    ccTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATcc                           <  1:4034952/50‑1 (MQ=255)                      ttCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCt                         >  1:1033021/1‑50 (MQ=255)                        cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCt                         <  1:57254/48‑1 (MQ=255)                        cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTg                        <  1:4557008/49‑1 (MQ=255)                         ggTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGc                       <  1:3685433/49‑1 (MQ=255)                         ggTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGc                       <  1:1426937/49‑1 (MQ=255)                         ggTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCt                      >  1:2782641/1‑50 (MQ=255)                          gTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTc                     <  1:5285345/50‑1 (MQ=255)                           tATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCg                    <  1:2699163/50‑1 (MQ=255)                            aTCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                   >  1:4630828/1‑50 (MQ=255)                             tCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGtt                  <  1:5582172/50‑1 (MQ=255)                             tCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                   >  1:3208316/1‑49 (MQ=255)                              cAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGtt                  >  1:4700611/1‑49 (MQ=255)                               aGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTaa                <  1:3065230/50‑1 (MQ=255)                               aGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 >  1:1157239/1‑49 (MQ=255)                               aGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 <  1:1625368/49‑1 (MQ=255)                                gATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTaaa               <  1:4335411/50‑1 (MQ=255)                                gATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTaaa               <  1:906243/50‑1 (MQ=255)                                gATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 >  1:2397133/1‑48 (MQ=255)                                 aTGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 >  1:2604282/1‑47 (MQ=255)                                 aTGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaag              <  1:5175826/50‑1 (MQ=255)                                  tGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaaga             <  1:3319654/50‑1 (MQ=255)                                   gCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaag              >  1:1175039/1‑48 (MQ=255)                                   gCGGCAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaag              >  1:1488025/1‑48 (MQ=255)                                    cGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaagaag           >  1:4760327/1‑50 (MQ=255)                                     ggTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc          <  1:3985059/50‑1 (MQ=255)                                     ggTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc          <  1:1388114/50‑1 (MQ=255)                                      gTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc          >  1:1979680/1‑49 (MQ=255)                                         aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGAt       <  1:3745021/49‑1 (MQ=255)                                            aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAg   <  1:1692996/50‑1 (MQ=255)                                            aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAg   <  1:5288863/50‑1 (MQ=255)                                            aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAg   <  1:748646/50‑1 (MQ=255)                                             gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAgc  <  1:1625126/50‑1 (MQ=255)                                             gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAgc  <  1:2903704/50‑1 (MQ=255)                                                |                                               GCGGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAGC  >  NC_000913/988452‑988545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑