BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	988,499	G→A	P163S (CCA→TCA)	asnS ←	asparagine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	988,499	0	G	A	100.0%	169.0 / NA	44	P163S (CCA→TCA)	asnS	asparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/29); total (15/29)

GCGGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAGC  >  NC_000913/988452‑988545

gcgGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACg                                              <  1:2003112/50‑1 (MQ=255)

 cgGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGt                                             <  1:23743/50‑1 (MQ=255)

  ggAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                            <  1:788197/50‑1 (MQ=255)

  ggAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                            >  1:681316/1‑50 (MQ=255)

   gAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTg                                           <  1:1789674/50‑1 (MQ=255)

    aaCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGa                                          <  1:4171781/50‑1 (MQ=255)

     aCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaa                                         <  1:3467904/50‑1 (MQ=255)

       aTTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAc                                       <  1:3077054/50‑1 (MQ=255)

        tttCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                      <  1:296204/50‑1 (MQ=255)

          tCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAccc                                     <  1:1193564/49‑1 (MQ=255)

          tCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                      >  1:4166085/1‑48 (MQ=255)

           cGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCa                                    <  1:1262342/49‑1 (MQ=255)

           cGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCa                                    <  1:732028/49‑1 (MQ=255)

            gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                   >  1:2529227/1‑49 (MQ=255)

                gCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAgaagaa                              >  1:619281/1‑50 (MQ=255)

                  aCCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATc                            <  1:2336360/50‑1 (MQ=255)

                  aCCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATc                            <  1:4151917/50‑1 (MQ=255)

                    cTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATccc                          >  1:2503812/1‑50 (MQ=255)

                    cTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATccc                          >  1:637967/1‑50 (MQ=255)

                          tATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCg                    <  1:3188247/50‑1 (MQ=255)

                            tCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                   <  1:2859971/49‑1 (MQ=255)

                            tCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                   >  1:3304481/1‑49 (MQ=255)

                              aGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 >  1:3059969/1‑49 (MQ=255)

                               gATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTaaa               <  1:281526/50‑1 (MQ=255)

                               gATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTaaa               <  1:2846568/50‑1 (MQ=255)

                               gATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 >  1:3699890/1‑48 (MQ=255)

                                aTGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaag              <  1:2086855/50‑1 (MQ=255)

                                 tGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaaga             <  1:1191133/50‑1 (MQ=255)

                                   cGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaagaag           <  1:2574130/50‑1 (MQ=255)

                                    ggTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc          <  1:2322615/50‑1 (MQ=255)

                                    ggTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc          <  1:4331906/50‑1 (MQ=255)

                                    ggTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc          >  1:1486418/1‑50 (MQ=255)

                                        aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGAt       <  1:2876040/49‑1 (MQ=255)

                                        aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATg      >  1:3993737/1‑50 (MQ=255)

                                        aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATg      <  1:3393199/50‑1 (MQ=255)

                                         tCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGACGCGATg      >  1:1581371/1‑49 (MQ=255)

                                         tCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGc     <  1:2086527/50‑1 (MQ=255)

                                           aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAg   >  1:410981/1‑50 (MQ=255)

                                           aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAg   <  1:2593901/50‑1 (MQ=255)

                                           aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAg   <  1:1762259/50‑1 (MQ=255)

                                           aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAg   >  1:885531/1‑50 (MQ=255)

                                            gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAgc  <  1:3084455/50‑1 (MQ=255)

                                            gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAgc  >  1:2038703/1‑50 (MQ=255)

                                            gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAgc  <  1:1362554/50‑1 (MQ=255)

GCGGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAGC  >  NC_000913/988452‑988545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`